Experiment: LB_DAP agar plate, outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14980 and BPHYT_RS14985 are separated by 134 nucleotides BPHYT_RS14985 and BPHYT_RS14990 overlap by 4 nucleotides BPHYT_RS14990 and BPHYT_RS14995 are separated by 14 nucleotides
BPHYT_RS14980: BPHYT_RS14980 - LysR family transcriptional regulator, at 3,404,858 to 3,405,937
_RS14980
BPHYT_RS14985: BPHYT_RS14985 - formate dehydrogenase subunit gamma, at 3,406,072 to 3,406,557
_RS14985
BPHYT_RS14990: BPHYT_RS14990 - formate dehydrogenase subunit beta, at 3,406,554 to 3,408,116
_RS14990
BPHYT_RS14995: BPHYT_RS14995 - formate dehydrogenase subunit alpha, at 3,408,131 to 3,411,079
_RS14995
Position (kb)
3406
3407
3408
3409 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3405.792 kb on + strand, within BPHYT_RS14980 at 3405.805 kb on + strand, within BPHYT_RS14980 at 3405.939 kb on + strand at 3405.939 kb on + strand at 3405.982 kb on - strand at 3405.982 kb on - strand at 3405.993 kb on - strand at 3405.993 kb on - strand at 3406.017 kb on + strand at 3406.018 kb on - strand at 3406.018 kb on - strand at 3406.177 kb on + strand, within BPHYT_RS14985 at 3406.177 kb on + strand, within BPHYT_RS14985 at 3406.178 kb on - strand, within BPHYT_RS14985 at 3406.255 kb on + strand, within BPHYT_RS14985 at 3406.255 kb on + strand, within BPHYT_RS14985 at 3406.255 kb on + strand, within BPHYT_RS14985 at 3406.256 kb on - strand, within BPHYT_RS14985 at 3406.256 kb on - strand, within BPHYT_RS14985 at 3406.259 kb on - strand, within BPHYT_RS14985 at 3406.259 kb on - strand, within BPHYT_RS14985 at 3406.356 kb on + strand, within BPHYT_RS14985 at 3406.566 kb on + strand at 3406.566 kb on + strand at 3406.566 kb on + strand at 3406.567 kb on - strand at 3406.567 kb on - strand at 3406.567 kb on - strand at 3406.567 kb on - strand at 3406.741 kb on - strand, within BPHYT_RS14990 at 3406.886 kb on + strand, within BPHYT_RS14990 at 3406.887 kb on - strand, within BPHYT_RS14990 at 3406.887 kb on - strand, within BPHYT_RS14990 at 3407.076 kb on + strand, within BPHYT_RS14990 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.149 kb on - strand, within BPHYT_RS14990 at 3407.149 kb on - strand, within BPHYT_RS14990 at 3407.208 kb on - strand, within BPHYT_RS14990 at 3407.208 kb on - strand, within BPHYT_RS14990 at 3407.211 kb on + strand, within BPHYT_RS14990 at 3407.211 kb on + strand, within BPHYT_RS14990 at 3407.211 kb on + strand, within BPHYT_RS14990 at 3407.212 kb on - strand, within BPHYT_RS14990 at 3407.212 kb on - strand, within BPHYT_RS14990 at 3407.352 kb on + strand, within BPHYT_RS14990 at 3407.352 kb on + strand, within BPHYT_RS14990 at 3407.353 kb on - strand, within BPHYT_RS14990 at 3407.357 kb on - strand, within BPHYT_RS14990 at 3407.397 kb on - strand, within BPHYT_RS14990 at 3407.526 kb on + strand, within BPHYT_RS14990 at 3407.715 kb on + strand, within BPHYT_RS14990 at 3407.715 kb on + strand, within BPHYT_RS14990 at 3407.715 kb on + strand, within BPHYT_RS14990 at 3408.088 kb on + strand at 3408.088 kb on + strand at 3408.089 kb on - strand at 3408.089 kb on - strand at 3408.089 kb on - strand at 3408.089 kb on - strand at 3408.187 kb on + strand at 3408.360 kb on + strand at 3408.361 kb on - strand at 3408.361 kb on - strand at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.541 kb on + strand, within BPHYT_RS14995 at 3408.541 kb on + strand, within BPHYT_RS14995 at 3408.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB remove 3,405,792 + BPHYT_RS14980 0.86 +0.0 3,405,805 + BPHYT_RS14980 0.88 -1.2 3,405,939 + +0.9 3,405,939 + -0.5 3,405,982 - +0.2 3,405,982 - +0.3 3,405,993 - -0.7 3,405,993 - +0.2 3,406,017 + +0.5 3,406,018 - +0.5 3,406,018 - +0.5 3,406,177 + BPHYT_RS14985 0.22 -0.0 3,406,177 + BPHYT_RS14985 0.22 -0.5 3,406,178 - BPHYT_RS14985 0.22 +0.2 3,406,255 + BPHYT_RS14985 0.38 -0.0 3,406,255 + BPHYT_RS14985 0.38 +0.2 3,406,255 + BPHYT_RS14985 0.38 -0.0 3,406,256 - BPHYT_RS14985 0.38 -0.4 3,406,256 - BPHYT_RS14985 0.38 +0.2 3,406,259 - BPHYT_RS14985 0.38 +1.5 3,406,259 - BPHYT_RS14985 0.38 +1.1 3,406,356 + BPHYT_RS14985 0.58 +0.6 3,406,566 + +0.4 3,406,566 + -0.4 3,406,566 + +0.1 3,406,567 - -0.2 3,406,567 - +0.1 3,406,567 - +0.0 3,406,567 - +0.5 3,406,741 - BPHYT_RS14990 0.12 -0.3 3,406,886 + BPHYT_RS14990 0.21 -1.1 3,406,887 - BPHYT_RS14990 0.21 -1.0 3,406,887 - BPHYT_RS14990 0.21 +0.1 3,407,076 + BPHYT_RS14990 0.33 +0.2 3,407,148 + BPHYT_RS14990 0.38 +0.8 3,407,148 + BPHYT_RS14990 0.38 -0.6 3,407,148 + BPHYT_RS14990 0.38 -0.7 3,407,148 + BPHYT_RS14990 0.38 +0.1 3,407,149 - BPHYT_RS14990 0.38 +0.2 3,407,149 - BPHYT_RS14990 0.38 -1.8 3,407,208 - BPHYT_RS14990 0.42 -0.1 3,407,208 - BPHYT_RS14990 0.42 -0.5 3,407,211 + BPHYT_RS14990 0.42 -0.5 3,407,211 + BPHYT_RS14990 0.42 -0.0 3,407,211 + BPHYT_RS14990 0.42 +0.3 3,407,212 - BPHYT_RS14990 0.42 -0.5 3,407,212 - BPHYT_RS14990 0.42 -2.0 3,407,352 + BPHYT_RS14990 0.51 -0.1 3,407,352 + BPHYT_RS14990 0.51 +0.3 3,407,353 - BPHYT_RS14990 0.51 +0.1 3,407,357 - BPHYT_RS14990 0.51 -0.2 3,407,397 - BPHYT_RS14990 0.54 -0.2 3,407,526 + BPHYT_RS14990 0.62 +0.1 3,407,715 + BPHYT_RS14990 0.74 +0.0 3,407,715 + BPHYT_RS14990 0.74 -1.3 3,407,715 + BPHYT_RS14990 0.74 -0.6 3,408,088 + +0.5 3,408,088 + +0.9 3,408,089 - -0.3 3,408,089 - +0.2 3,408,089 - +0.2 3,408,089 - -1.4 3,408,187 + +0.7 3,408,360 + -0.5 3,408,361 - +0.9 3,408,361 - -0.8 3,408,539 + BPHYT_RS14995 0.14 +0.4 3,408,539 + BPHYT_RS14995 0.14 -0.3 3,408,539 + BPHYT_RS14995 0.14 -1.8 3,408,539 + +2.3 3,408,539 + BPHYT_RS14995 0.14 +1.0 3,408,539 + BPHYT_RS14995 0.14 +0.6 3,408,539 + BPHYT_RS14995 0.14 -0.4 3,408,539 + BPHYT_RS14995 0.14 +0.3 3,408,539 + BPHYT_RS14995 0.14 -0.6 3,408,540 - BPHYT_RS14995 0.14 +0.5 3,408,540 - BPHYT_RS14995 0.14 +0.8 3,408,540 - BPHYT_RS14995 0.14 +0.2 3,408,540 - BPHYT_RS14995 0.14 -0.6 3,408,541 + BPHYT_RS14995 0.14 -1.5 3,408,541 + BPHYT_RS14995 0.14 +1.0 3,408,541 + -1.0
Or see this region's nucleotide sequence