Experiment: LB_DAP agar plate, outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13910 and BPHYT_RS13915 overlap by 4 nucleotides BPHYT_RS13915 and BPHYT_RS13920 are separated by 10 nucleotides BPHYT_RS13920 and BPHYT_RS13925 overlap by 4 nucleotides
BPHYT_RS13910: BPHYT_RS13910 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 3,175,184 to 3,177,229
_RS13910
BPHYT_RS13915: BPHYT_RS13915 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, at 3,177,226 to 3,179,199
_RS13915
BPHYT_RS13920: BPHYT_RS13920 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data) , at 3,179,210 to 3,180,127
_RS13920
BPHYT_RS13925: BPHYT_RS13925 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data) , at 3,180,124 to 3,180,924
_RS13925
Position (kb)
3177
3178
3179
3180 Strain fitness (log2 ratio)
-1
0
1
2 at 3176.654 kb on - strand, within BPHYT_RS13910 at 3176.847 kb on - strand, within BPHYT_RS13910 at 3177.240 kb on - strand at 3177.683 kb on + strand, within BPHYT_RS13915 at 3178.024 kb on + strand, within BPHYT_RS13915 at 3178.723 kb on + strand at 3179.009 kb on - strand at 3179.009 kb on - strand at 3179.230 kb on + strand at 3179.399 kb on + strand, within BPHYT_RS13920 at 3179.399 kb on + strand, within BPHYT_RS13920 at 3179.426 kb on + strand, within BPHYT_RS13920 at 3179.493 kb on + strand, within BPHYT_RS13920 at 3179.525 kb on + strand, within BPHYT_RS13920 at 3179.612 kb on + strand, within BPHYT_RS13920 at 3179.613 kb on - strand, within BPHYT_RS13920 at 3179.644 kb on + strand, within BPHYT_RS13920 at 3179.644 kb on + strand, within BPHYT_RS13920
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB remove 3,176,654 - BPHYT_RS13910 0.72 +2.2 3,176,847 - BPHYT_RS13910 0.81 +0.1 3,177,240 - +0.8 3,177,683 + BPHYT_RS13915 0.23 +1.3 3,178,024 + BPHYT_RS13915 0.40 +0.4 3,178,723 + +1.9 3,179,009 - -0.3 3,179,009 - +0.3 3,179,230 + +0.7 3,179,399 + BPHYT_RS13920 0.21 -1.5 3,179,399 + BPHYT_RS13920 0.21 -0.0 3,179,426 + BPHYT_RS13920 0.24 +0.7 3,179,493 + BPHYT_RS13920 0.31 -0.2 3,179,525 + BPHYT_RS13920 0.34 +0.1 3,179,612 + BPHYT_RS13920 0.44 -1.5 3,179,613 - BPHYT_RS13920 0.44 -0.3 3,179,644 + BPHYT_RS13920 0.47 -0.9 3,179,644 + BPHYT_RS13920 0.47 +0.1
Or see this region's nucleotide sequence