Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13530

Experiment: LB_DAP agar plate, outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13520 and BPHYT_RS13525 are separated by 17 nucleotidesBPHYT_RS13525 and BPHYT_RS13530 are separated by 94 nucleotidesBPHYT_RS13530 and BPHYT_RS13535 are separated by 12 nucleotidesBPHYT_RS13535 and BPHYT_RS13540 are separated by 62 nucleotides BPHYT_RS13520: BPHYT_RS13520 - isopropylmalate isomerase, at 3,084,454 to 3,085,869 _RS13520 BPHYT_RS13525: BPHYT_RS13525 - alpha/beta hydrolase, at 3,085,887 to 3,086,765 _RS13525 BPHYT_RS13530: BPHYT_RS13530 - LysR family transcriptional regulator, at 3,086,860 to 3,087,762 _RS13530 BPHYT_RS13535: BPHYT_RS13535 - hypothetical protein, at 3,087,775 to 3,087,966 _RS13535 BPHYT_RS13540: BPHYT_RS13540 - LysR family transcriptional regulator, at 3,088,029 to 3,088,937 _RS13540 Position (kb) 3086 3087 3088Strain fitness (log2 ratio) -2 -1 0 1at 3085.948 kb on + strandat 3085.948 kb on + strandat 3086.793 kb on + strandat 3086.794 kb on - strandat 3087.790 kb on - strandat 3087.991 kb on + strandat 3087.992 kb on - strandat 3088.121 kb on + strand, within BPHYT_RS13540at 3088.122 kb on - strand, within BPHYT_RS13540at 3088.123 kb on + strand, within BPHYT_RS13540at 3088.124 kb on - strand, within BPHYT_RS13540at 3088.124 kb on - strand, within BPHYT_RS13540at 3088.124 kb on - strand, within BPHYT_RS13540at 3088.124 kb on - strand, within BPHYT_RS13540at 3088.124 kb on - strand, within BPHYT_RS13540at 3088.132 kb on + strand, within BPHYT_RS13540at 3088.132 kb on + strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.133 kb on - strand, within BPHYT_RS13540at 3088.216 kb on + strand, within BPHYT_RS13540at 3088.217 kb on - strand, within BPHYT_RS13540at 3088.217 kb on - strand, within BPHYT_RS13540at 3088.217 kb on - strand, within BPHYT_RS13540at 3088.331 kb on - strand, within BPHYT_RS13540at 3088.454 kb on - strand, within BPHYT_RS13540at 3088.745 kb on - strand, within BPHYT_RS13540

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB
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3,085,948 + +0.9
3,085,948 + -0.2
3,086,793 + -0.7
3,086,794 - +1.1
3,087,790 - -0.2
3,087,991 + -2.4
3,087,992 - -0.6
3,088,121 + BPHYT_RS13540 0.10 -0.1
3,088,122 - BPHYT_RS13540 0.10 +0.1
3,088,123 + BPHYT_RS13540 0.10 -1.6
3,088,124 - BPHYT_RS13540 0.10 -0.4
3,088,124 - BPHYT_RS13540 0.10 +0.0
3,088,124 - BPHYT_RS13540 0.10 +0.2
3,088,124 - BPHYT_RS13540 0.10 +0.1
3,088,124 - BPHYT_RS13540 0.10 -0.1
3,088,132 + BPHYT_RS13540 0.11 +0.1
3,088,132 + BPHYT_RS13540 0.11 -0.1
3,088,133 - BPHYT_RS13540 0.11 +0.5
3,088,133 - BPHYT_RS13540 0.11 -0.2
3,088,133 - BPHYT_RS13540 0.11 -0.6
3,088,133 - BPHYT_RS13540 0.11 -0.6
3,088,133 - BPHYT_RS13540 0.11 +1.1
3,088,133 - BPHYT_RS13540 0.11 +0.1
3,088,133 - BPHYT_RS13540 0.11 +0.5
3,088,133 - BPHYT_RS13540 0.11 +0.3
3,088,216 + BPHYT_RS13540 0.21 +0.4
3,088,217 - BPHYT_RS13540 0.21 +0.0
3,088,217 - BPHYT_RS13540 0.21 -0.3
3,088,217 - BPHYT_RS13540 0.21 +0.2
3,088,331 - BPHYT_RS13540 0.33 +0.0
3,088,454 - BPHYT_RS13540 0.47 +0.4
3,088,745 - BPHYT_RS13540 0.79 -0.0

Or see this region's nucleotide sequence