Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09060

Experiment: LB_DAP agar plate, outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09055 and BPHYT_RS09060 are separated by 67 nucleotidesBPHYT_RS09060 and BPHYT_RS09065 overlap by 17 nucleotidesBPHYT_RS09065 and BPHYT_RS09070 are separated by 23 nucleotides BPHYT_RS09055: BPHYT_RS09055 - DNA polymerase III subunit gamma/tau, at 2,049,245 to 2,051,926 _RS09055 BPHYT_RS09060: BPHYT_RS09060 - hypothetical protein, at 2,051,994 to 2,052,320 _RS09060 BPHYT_RS09065: BPHYT_RS09065 - hypothetical protein, at 2,052,304 to 2,052,570 _RS09065 BPHYT_RS09070: BPHYT_RS09070 - recombination protein RecR, at 2,052,594 to 2,053,190 _RS09070 Position (kb) 2051 2052 2053Strain fitness (log2 ratio) -1 0 1at 2051.884 kb on + strandat 2052.571 kb on + strandat 2052.903 kb on + strand, within BPHYT_RS09070at 2052.903 kb on + strand, within BPHYT_RS09070at 2052.904 kb on - strand, within BPHYT_RS09070at 2053.056 kb on + strand, within BPHYT_RS09070at 2053.057 kb on - strand, within BPHYT_RS09070at 2053.057 kb on - strand, within BPHYT_RS09070at 2053.137 kb on + strandat 2053.220 kb on + strandat 2053.220 kb on + strandat 2053.221 kb on - strandat 2053.252 kb on + strandat 2053.252 kb on + strandat 2053.253 kb on - strandat 2053.253 kb on - strandat 2053.313 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB
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2,051,884 + -0.5
2,052,571 + -0.4
2,052,903 + BPHYT_RS09070 0.52 +0.6
2,052,903 + BPHYT_RS09070 0.52 -0.3
2,052,904 - BPHYT_RS09070 0.52 +0.5
2,053,056 + BPHYT_RS09070 0.77 -0.1
2,053,057 - BPHYT_RS09070 0.78 +1.2
2,053,057 - BPHYT_RS09070 0.78 -1.0
2,053,137 + -0.5
2,053,220 + +0.1
2,053,220 + -0.7
2,053,221 - -0.2
2,053,252 + +0.5
2,053,252 + -0.4
2,053,253 - +1.0
2,053,253 - +0.1
2,053,313 + +0.4

Or see this region's nucleotide sequence