Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27940 and BWI76_RS27945 overlap by 17 nucleotides BWI76_RS27945 and BWI76_RS27950 overlap by 4 nucleotides BWI76_RS27950 and BWI76_RS27955 are separated by 243 nucleotides
BWI76_RS27940: BWI76_RS27940 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 5,794,702 to 5,795,319
_RS27940
BWI76_RS27945: BWI76_RS27945 - 2-oxo-3-deoxygalactonate kinase, at 5,795,303 to 5,796,178
_RS27945
BWI76_RS27950: BWI76_RS27950 - transcriptional regulator, at 5,796,175 to 5,796,864
_RS27950
BWI76_RS27955: BWI76_RS27955 - sugar-phosphatase, at 5,797,108 to 5,797,920
_RS27955
Position (kb) 5796
5797 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5795.276 kb on - strand at 5795.291 kb on - strand at 5795.330 kb on + strand at 5795.338 kb on - strand at 5795.409 kb on - strand, within BWI76_RS27945 at 5795.707 kb on - strand, within BWI76_RS27945 at 5795.707 kb on - strand, within BWI76_RS27945 at 5795.815 kb on + strand, within BWI76_RS27945 at 5796.152 kb on + strand at 5796.328 kb on - strand, within BWI76_RS27950 at 5796.356 kb on - strand, within BWI76_RS27950 at 5796.381 kb on + strand, within BWI76_RS27950 at 5796.791 kb on - strand, within BWI76_RS27950 at 5796.828 kb on + strand at 5796.860 kb on + strand at 5796.891 kb on - strand at 5797.154 kb on + strand at 5797.207 kb on + strand, within BWI76_RS27955 at 5797.460 kb on + strand, within BWI76_RS27955 at 5797.460 kb on + strand, within BWI76_RS27955 at 5797.468 kb on - strand, within BWI76_RS27955 at 5797.544 kb on - strand, within BWI76_RS27955 at 5797.603 kb on - strand, within BWI76_RS27955 at 5797.662 kb on - strand, within BWI76_RS27955 at 5797.701 kb on + strand, within BWI76_RS27955 at 5797.795 kb on + strand, within BWI76_RS27955
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,795,276 - -0.3 5,795,291 - +0.8 5,795,330 + +0.1 5,795,338 - +2.8 5,795,409 - BWI76_RS27945 0.12 +1.3 5,795,707 - BWI76_RS27945 0.46 -0.4 5,795,707 - BWI76_RS27945 0.46 -0.4 5,795,815 + BWI76_RS27945 0.58 +0.2 5,796,152 + +0.7 5,796,328 - BWI76_RS27950 0.22 -0.6 5,796,356 - BWI76_RS27950 0.26 -0.7 5,796,381 + BWI76_RS27950 0.30 -1.0 5,796,791 - BWI76_RS27950 0.89 -1.7 5,796,828 + -1.4 5,796,860 + +0.2 5,796,891 - +0.2 5,797,154 + +1.2 5,797,207 + BWI76_RS27955 0.12 +0.1 5,797,460 + BWI76_RS27955 0.43 -1.5 5,797,460 + BWI76_RS27955 0.43 -0.1 5,797,468 - BWI76_RS27955 0.44 -0.8 5,797,544 - BWI76_RS27955 0.54 -0.6 5,797,603 - BWI76_RS27955 0.61 +0.2 5,797,662 - BWI76_RS27955 0.68 -2.0 5,797,701 + BWI76_RS27955 0.73 -0.2 5,797,795 + BWI76_RS27955 0.85 -1.5
Or see this region's nucleotide sequence