Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27840 and BWI76_RS27845 are separated by 177 nucleotides BWI76_RS27845 and BWI76_RS27850 are separated by 245 nucleotides
BWI76_RS27840: BWI76_RS27840 - putative membrane protein, transport, at 5,773,786 to 5,774,619
_RS27840
BWI76_RS27845: BWI76_RS27845 - multidrug transporter EmrD, at 5,774,797 to 5,775,981
_RS27845
BWI76_RS27850: BWI76_RS27850 - DNA-binding transcriptional regulator DsdC, at 5,776,227 to 5,777,156
_RS27850
Position (kb) 5774
5775
5776 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5773.817 kb on + strand at 5773.898 kb on + strand, within BWI76_RS27840 at 5773.929 kb on + strand, within BWI76_RS27840 at 5773.951 kb on - strand, within BWI76_RS27840 at 5774.008 kb on - strand, within BWI76_RS27840 at 5774.029 kb on - strand, within BWI76_RS27840 at 5774.184 kb on - strand, within BWI76_RS27840 at 5774.195 kb on + strand, within BWI76_RS27840 at 5774.203 kb on - strand, within BWI76_RS27840 at 5774.256 kb on + strand, within BWI76_RS27840 at 5774.297 kb on + strand, within BWI76_RS27840 at 5774.303 kb on - strand, within BWI76_RS27840 at 5774.305 kb on - strand, within BWI76_RS27840 at 5774.343 kb on - strand, within BWI76_RS27840 at 5774.461 kb on + strand, within BWI76_RS27840 at 5774.773 kb on + strand at 5774.793 kb on - strand at 5774.976 kb on + strand, within BWI76_RS27845 at 5775.074 kb on + strand, within BWI76_RS27845 at 5775.109 kb on + strand, within BWI76_RS27845 at 5775.176 kb on - strand, within BWI76_RS27845 at 5775.181 kb on + strand, within BWI76_RS27845 at 5775.183 kb on + strand, within BWI76_RS27845 at 5775.183 kb on + strand, within BWI76_RS27845 at 5775.192 kb on + strand, within BWI76_RS27845 at 5775.200 kb on - strand, within BWI76_RS27845 at 5775.200 kb on - strand, within BWI76_RS27845 at 5775.268 kb on + strand, within BWI76_RS27845 at 5775.370 kb on + strand, within BWI76_RS27845 at 5775.378 kb on - strand, within BWI76_RS27845 at 5775.403 kb on - strand, within BWI76_RS27845 at 5775.513 kb on - strand, within BWI76_RS27845 at 5776.186 kb on - strand at 5776.308 kb on + strand at 5776.323 kb on + strand, within BWI76_RS27850 at 5776.503 kb on + strand, within BWI76_RS27850 at 5776.799 kb on + strand, within BWI76_RS27850 at 5776.804 kb on + strand, within BWI76_RS27850 at 5776.804 kb on + strand, within BWI76_RS27850 at 5776.812 kb on - strand, within BWI76_RS27850
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,773,817 + -0.2 5,773,898 + BWI76_RS27840 0.13 -0.2 5,773,929 + BWI76_RS27840 0.17 +0.2 5,773,951 - BWI76_RS27840 0.20 -0.9 5,774,008 - BWI76_RS27840 0.27 -0.1 5,774,029 - BWI76_RS27840 0.29 -0.4 5,774,184 - BWI76_RS27840 0.48 +1.5 5,774,195 + BWI76_RS27840 0.49 +0.2 5,774,203 - BWI76_RS27840 0.50 -1.2 5,774,256 + BWI76_RS27840 0.56 -0.5 5,774,297 + BWI76_RS27840 0.61 -1.6 5,774,303 - BWI76_RS27840 0.62 -0.1 5,774,305 - BWI76_RS27840 0.62 +2.0 5,774,343 - BWI76_RS27840 0.67 +0.7 5,774,461 + BWI76_RS27840 0.81 +1.7 5,774,773 + -1.1 5,774,793 - +0.9 5,774,976 + BWI76_RS27845 0.15 +0.3 5,775,074 + BWI76_RS27845 0.23 -1.8 5,775,109 + BWI76_RS27845 0.26 +0.8 5,775,176 - BWI76_RS27845 0.32 -0.5 5,775,181 + BWI76_RS27845 0.32 +1.1 5,775,183 + BWI76_RS27845 0.33 -0.1 5,775,183 + BWI76_RS27845 0.33 -1.0 5,775,192 + BWI76_RS27845 0.33 +1.5 5,775,200 - BWI76_RS27845 0.34 +1.8 5,775,200 - BWI76_RS27845 0.34 +1.8 5,775,268 + BWI76_RS27845 0.40 -0.0 5,775,370 + BWI76_RS27845 0.48 +1.5 5,775,378 - BWI76_RS27845 0.49 +0.2 5,775,403 - BWI76_RS27845 0.51 -0.7 5,775,513 - BWI76_RS27845 0.60 +0.5 5,776,186 - -0.0 5,776,308 + +0.4 5,776,323 + BWI76_RS27850 0.10 +1.9 5,776,503 + BWI76_RS27850 0.30 -0.1 5,776,799 + BWI76_RS27850 0.62 -0.8 5,776,804 + BWI76_RS27850 0.62 -0.2 5,776,804 + BWI76_RS27850 0.62 +1.7 5,776,812 - BWI76_RS27850 0.63 +1.1
Or see this region's nucleotide sequence