Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26885 and BWI76_RS26890 are separated by 98 nucleotides BWI76_RS26890 and BWI76_RS26895 are separated by 429 nucleotides BWI76_RS26895 and BWI76_RS26900 are separated by 89 nucleotides
BWI76_RS26885: BWI76_RS26885 - ABC transporter substrate-binding protein, at 5,577,554 to 5,579,161
_RS26885
BWI76_RS26890: BWI76_RS26890 - hypothetical protein, at 5,579,260 to 5,579,502
_RS26890
BWI76_RS26895: BWI76_RS26895 - tRNA-Pro, at 5,579,932 to 5,580,008
_RS26895
BWI76_RS26900: BWI76_RS26900 - phosphoethanolamine transferase, at 5,580,098 to 5,581,786
_RS26900
Position (kb) 5579
5580 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 5578.284 kb on + strand, within BWI76_RS26885 at 5578.302 kb on - strand, within BWI76_RS26885 at 5578.314 kb on + strand, within BWI76_RS26885 at 5578.406 kb on - strand, within BWI76_RS26885 at 5578.414 kb on + strand, within BWI76_RS26885 at 5578.422 kb on - strand, within BWI76_RS26885 at 5578.422 kb on - strand, within BWI76_RS26885 at 5578.487 kb on - strand, within BWI76_RS26885 at 5578.536 kb on - strand, within BWI76_RS26885 at 5578.536 kb on - strand, within BWI76_RS26885 at 5578.580 kb on - strand, within BWI76_RS26885 at 5578.668 kb on + strand, within BWI76_RS26885 at 5578.711 kb on + strand, within BWI76_RS26885 at 5578.715 kb on + strand, within BWI76_RS26885 at 5578.877 kb on - strand, within BWI76_RS26885 at 5578.879 kb on + strand, within BWI76_RS26885 at 5578.879 kb on + strand, within BWI76_RS26885 at 5578.885 kb on + strand, within BWI76_RS26885 at 5578.885 kb on + strand, within BWI76_RS26885 at 5578.886 kb on + strand, within BWI76_RS26885 at 5578.894 kb on - strand, within BWI76_RS26885 at 5578.894 kb on - strand, within BWI76_RS26885 at 5578.894 kb on - strand, within BWI76_RS26885 at 5578.942 kb on + strand, within BWI76_RS26885 at 5578.987 kb on + strand, within BWI76_RS26885 at 5578.995 kb on - strand, within BWI76_RS26885 at 5578.995 kb on - strand, within BWI76_RS26885 at 5579.019 kb on - strand at 5579.032 kb on + strand at 5579.040 kb on - strand at 5579.080 kb on + strand at 5579.088 kb on - strand at 5579.088 kb on - strand at 5579.100 kb on - strand at 5579.121 kb on - strand at 5579.121 kb on - strand at 5579.122 kb on - strand at 5579.124 kb on + strand at 5579.132 kb on - strand at 5579.193 kb on + strand at 5579.230 kb on + strand at 5579.238 kb on - strand at 5579.247 kb on + strand at 5579.257 kb on - strand at 5579.267 kb on - strand at 5579.270 kb on - strand at 5579.303 kb on - strand, within BWI76_RS26890 at 5579.476 kb on - strand, within BWI76_RS26890 at 5579.715 kb on + strand at 5579.715 kb on + strand at 5579.715 kb on + strand at 5579.715 kb on + strand at 5579.723 kb on - strand at 5579.725 kb on - strand at 5579.753 kb on + strand at 5579.867 kb on + strand at 5580.174 kb on - strand at 5580.183 kb on - strand at 5580.193 kb on - strand at 5580.193 kb on - strand at 5580.272 kb on - strand, within BWI76_RS26900 at 5580.497 kb on - strand, within BWI76_RS26900
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,578,284 + BWI76_RS26885 0.45 -0.5 5,578,302 - BWI76_RS26885 0.47 +2.0 5,578,314 + BWI76_RS26885 0.47 +0.1 5,578,406 - BWI76_RS26885 0.53 +1.3 5,578,414 + BWI76_RS26885 0.53 +0.3 5,578,422 - BWI76_RS26885 0.54 -0.2 5,578,422 - BWI76_RS26885 0.54 -0.7 5,578,487 - BWI76_RS26885 0.58 +0.3 5,578,536 - BWI76_RS26885 0.61 +0.8 5,578,536 - BWI76_RS26885 0.61 +1.8 5,578,580 - BWI76_RS26885 0.64 +0.8 5,578,668 + BWI76_RS26885 0.69 +0.3 5,578,711 + BWI76_RS26885 0.72 +0.3 5,578,715 + BWI76_RS26885 0.72 -0.7 5,578,877 - BWI76_RS26885 0.82 -1.5 5,578,879 + BWI76_RS26885 0.82 +0.2 5,578,879 + BWI76_RS26885 0.82 +1.1 5,578,885 + BWI76_RS26885 0.83 +1.1 5,578,885 + BWI76_RS26885 0.83 +0.2 5,578,886 + BWI76_RS26885 0.83 -1.1 5,578,894 - BWI76_RS26885 0.83 -0.2 5,578,894 - BWI76_RS26885 0.83 -1.4 5,578,894 - BWI76_RS26885 0.83 +0.6 5,578,942 + BWI76_RS26885 0.86 -1.5 5,578,987 + BWI76_RS26885 0.89 -0.2 5,578,995 - BWI76_RS26885 0.90 +0.2 5,578,995 - BWI76_RS26885 0.90 +0.6 5,579,019 - +0.3 5,579,032 + -0.2 5,579,040 - +0.6 5,579,080 + +1.0 5,579,088 - -0.7 5,579,088 - -0.2 5,579,100 - -0.4 5,579,121 - -0.8 5,579,121 - +2.0 5,579,122 - +1.8 5,579,124 + +3.0 5,579,132 - +4.6 5,579,193 + -0.0 5,579,230 + +1.2 5,579,238 - +0.3 5,579,247 + -0.7 5,579,257 - -1.3 5,579,267 - -1.7 5,579,270 - -1.5 5,579,303 - BWI76_RS26890 0.18 -0.9 5,579,476 - BWI76_RS26890 0.89 -0.7 5,579,715 + +0.8 5,579,715 + -0.8 5,579,715 + +0.3 5,579,715 + +0.9 5,579,723 - +0.8 5,579,725 - +0.5 5,579,753 + -1.1 5,579,867 + +0.8 5,580,174 - +0.0 5,580,183 - -0.1 5,580,193 - -0.6 5,580,193 - -1.1 5,580,272 - BWI76_RS26900 0.10 +1.0 5,580,497 - BWI76_RS26900 0.24 -1.1
Or see this region's nucleotide sequence