Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26735 and BWI76_RS26740 are separated by 49 nucleotides BWI76_RS26740 and BWI76_RS26745 overlap by 32 nucleotides BWI76_RS26745 and BWI76_RS26750 overlap by 8 nucleotides
BWI76_RS26735: BWI76_RS26735 - MFS transporter, at 5,536,026 to 5,537,348
_RS26735
BWI76_RS26740: BWI76_RS26740 - AsmA family protein, at 5,537,398 to 5,539,449
_RS26740
BWI76_RS26745: BWI76_RS26745 - antibiotic biosynthesis monooxygenase, at 5,539,418 to 5,539,652
_RS26745
BWI76_RS26750: BWI76_RS26750 - PfkB domain-containing protein, at 5,539,645 to 5,540,580
_RS26750
Position (kb) 5537
5538
5539
5540 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 5536.406 kb on + strand, within BWI76_RS26735 at 5536.668 kb on - strand, within BWI76_RS26735 at 5536.727 kb on + strand, within BWI76_RS26735 at 5536.789 kb on + strand, within BWI76_RS26735 at 5536.822 kb on - strand, within BWI76_RS26735 at 5536.822 kb on - strand, within BWI76_RS26735 at 5536.984 kb on + strand, within BWI76_RS26735 at 5536.996 kb on + strand, within BWI76_RS26735 at 5537.000 kb on - strand, within BWI76_RS26735 at 5537.004 kb on - strand, within BWI76_RS26735 at 5537.014 kb on + strand, within BWI76_RS26735 at 5537.277 kb on - strand at 5537.350 kb on - strand at 5537.452 kb on - strand at 5537.461 kb on - strand at 5537.510 kb on - strand at 5537.615 kb on + strand, within BWI76_RS26740 at 5537.904 kb on - strand, within BWI76_RS26740 at 5537.934 kb on + strand, within BWI76_RS26740 at 5538.058 kb on + strand, within BWI76_RS26740 at 5538.122 kb on + strand, within BWI76_RS26740 at 5538.156 kb on - strand at 5538.179 kb on - strand, within BWI76_RS26740 at 5538.293 kb on + strand, within BWI76_RS26740 at 5538.293 kb on + strand, within BWI76_RS26740 at 5538.546 kb on - strand, within BWI76_RS26740 at 5538.895 kb on + strand, within BWI76_RS26740 at 5538.903 kb on - strand, within BWI76_RS26740 at 5539.057 kb on + strand, within BWI76_RS26740 at 5539.369 kb on + strand at 5539.450 kb on - strand, within BWI76_RS26745 at 5539.452 kb on - strand, within BWI76_RS26745 at 5539.452 kb on - strand, within BWI76_RS26745 at 5539.479 kb on + strand, within BWI76_RS26745 at 5539.602 kb on + strand, within BWI76_RS26745 at 5539.610 kb on - strand, within BWI76_RS26745 at 5539.610 kb on - strand, within BWI76_RS26745 at 5539.614 kb on - strand, within BWI76_RS26745 at 5539.615 kb on - strand, within BWI76_RS26745 at 5539.732 kb on + strand at 5539.900 kb on + strand, within BWI76_RS26750 at 5539.900 kb on + strand, within BWI76_RS26750 at 5540.003 kb on - strand, within BWI76_RS26750 at 5540.009 kb on + strand, within BWI76_RS26750 at 5540.024 kb on - strand, within BWI76_RS26750 at 5540.295 kb on - strand, within BWI76_RS26750 at 5540.295 kb on - strand, within BWI76_RS26750
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,536,406 + BWI76_RS26735 0.29 +0.1 5,536,668 - BWI76_RS26735 0.49 +0.2 5,536,727 + BWI76_RS26735 0.53 -0.2 5,536,789 + BWI76_RS26735 0.58 -1.0 5,536,822 - BWI76_RS26735 0.60 -0.7 5,536,822 - BWI76_RS26735 0.60 -0.5 5,536,984 + BWI76_RS26735 0.72 +1.0 5,536,996 + BWI76_RS26735 0.73 -0.4 5,537,000 - BWI76_RS26735 0.74 +0.1 5,537,004 - BWI76_RS26735 0.74 +0.5 5,537,014 + BWI76_RS26735 0.75 +0.2 5,537,277 - -1.3 5,537,350 - +1.3 5,537,452 - -1.2 5,537,461 - -1.5 5,537,510 - +0.3 5,537,615 + BWI76_RS26740 0.11 -0.9 5,537,904 - BWI76_RS26740 0.25 -0.0 5,537,934 + BWI76_RS26740 0.26 +1.1 5,538,058 + BWI76_RS26740 0.32 +0.0 5,538,122 + BWI76_RS26740 0.35 +1.3 5,538,156 - -0.2 5,538,179 - BWI76_RS26740 0.38 +0.6 5,538,293 + BWI76_RS26740 0.44 +1.2 5,538,293 + BWI76_RS26740 0.44 +0.2 5,538,546 - BWI76_RS26740 0.56 +1.3 5,538,895 + BWI76_RS26740 0.73 +2.0 5,538,903 - BWI76_RS26740 0.73 -0.2 5,539,057 + BWI76_RS26740 0.81 +1.4 5,539,369 + +4.0 5,539,450 - BWI76_RS26745 0.14 +4.8 5,539,452 - BWI76_RS26745 0.14 -0.1 5,539,452 - BWI76_RS26745 0.14 -0.9 5,539,479 + BWI76_RS26745 0.26 -0.6 5,539,602 + BWI76_RS26745 0.78 -0.5 5,539,610 - BWI76_RS26745 0.82 -0.1 5,539,610 - BWI76_RS26745 0.82 +4.2 5,539,614 - BWI76_RS26745 0.83 -0.8 5,539,615 - BWI76_RS26745 0.84 -0.3 5,539,732 + -0.7 5,539,900 + BWI76_RS26750 0.27 +0.3 5,539,900 + BWI76_RS26750 0.27 -1.4 5,540,003 - BWI76_RS26750 0.38 -0.1 5,540,009 + BWI76_RS26750 0.39 +0.0 5,540,024 - BWI76_RS26750 0.40 -1.9 5,540,295 - BWI76_RS26750 0.69 -0.3 5,540,295 - BWI76_RS26750 0.69 -0.6
Or see this region's nucleotide sequence