Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26325 and BWI76_RS26330 are separated by 44 nucleotides BWI76_RS26330 and BWI76_RS26335 are separated by 13 nucleotides BWI76_RS26335 and BWI76_RS26340 overlap by 4 nucleotides
BWI76_RS26325: BWI76_RS26325 - altronate hydrolase, at 5,448,974 to 5,450,569
_RS26325
BWI76_RS26330: BWI76_RS26330 - ABC transporter ATP-binding protein, at 5,450,614 to 5,451,315
_RS26330
BWI76_RS26335: BWI76_RS26335 - ABC transporter ATP-binding protein, at 5,451,329 to 5,452,096
_RS26335
BWI76_RS26340: BWI76_RS26340 - branched-chain amino acid ABC transporter permease, at 5,452,093 to 5,453,373
_RS26340
Position (kb) 5451
5452
5453 Strain fitness (log2 ratio) -1
0
1
2 at 5450.506 kb on + strand at 5450.528 kb on - strand at 5450.839 kb on - strand, within BWI76_RS26330 at 5450.951 kb on + strand, within BWI76_RS26330 at 5451.036 kb on - strand, within BWI76_RS26330 at 5451.133 kb on + strand, within BWI76_RS26330 at 5451.230 kb on + strand, within BWI76_RS26330 at 5451.290 kb on + strand at 5451.407 kb on + strand, within BWI76_RS26335 at 5451.472 kb on + strand, within BWI76_RS26335 at 5451.591 kb on + strand, within BWI76_RS26335 at 5451.738 kb on - strand, within BWI76_RS26335 at 5452.303 kb on - strand, within BWI76_RS26340 at 5452.311 kb on - strand, within BWI76_RS26340 at 5452.353 kb on - strand, within BWI76_RS26340 at 5452.518 kb on - strand, within BWI76_RS26340 at 5452.548 kb on + strand at 5452.574 kb on + strand, within BWI76_RS26340 at 5452.712 kb on - strand, within BWI76_RS26340 at 5452.712 kb on - strand, within BWI76_RS26340 at 5452.844 kb on + strand, within BWI76_RS26340 at 5452.885 kb on - strand, within BWI76_RS26340 at 5452.951 kb on + strand, within BWI76_RS26340 at 5452.992 kb on + strand, within BWI76_RS26340 at 5453.083 kb on - strand, within BWI76_RS26340
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,450,506 + -0.4 5,450,528 - +0.7 5,450,839 - BWI76_RS26330 0.32 +1.6 5,450,951 + BWI76_RS26330 0.48 +0.4 5,451,036 - BWI76_RS26330 0.60 +0.0 5,451,133 + BWI76_RS26330 0.74 +1.9 5,451,230 + BWI76_RS26330 0.88 +0.2 5,451,290 + +0.2 5,451,407 + BWI76_RS26335 0.10 +0.2 5,451,472 + BWI76_RS26335 0.19 -0.2 5,451,591 + BWI76_RS26335 0.34 +1.3 5,451,738 - BWI76_RS26335 0.53 -0.0 5,452,303 - BWI76_RS26340 0.16 +0.2 5,452,311 - BWI76_RS26340 0.17 +1.1 5,452,353 - BWI76_RS26340 0.20 -0.2 5,452,518 - BWI76_RS26340 0.33 -1.0 5,452,548 + +1.5 5,452,574 + BWI76_RS26340 0.38 -0.6 5,452,712 - BWI76_RS26340 0.48 -0.5 5,452,712 - BWI76_RS26340 0.48 -1.5 5,452,844 + BWI76_RS26340 0.59 +0.3 5,452,885 - BWI76_RS26340 0.62 -0.4 5,452,951 + BWI76_RS26340 0.67 -0.1 5,452,992 + BWI76_RS26340 0.70 -1.5 5,453,083 - BWI76_RS26340 0.77 -1.4
Or see this region's nucleotide sequence