Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25890 and BWI76_RS25895 are separated by 7 nucleotides BWI76_RS25895 and BWI76_RS25900 overlap by 1 nucleotides BWI76_RS25900 and BWI76_RS25905 overlap by 1 nucleotides BWI76_RS25905 and BWI76_RS25910 are separated by 166 nucleotides
BWI76_RS25890: BWI76_RS25890 - sulfurtransferase TusB, at 5,356,199 to 5,356,486
_RS25890
BWI76_RS25895: BWI76_RS25895 - sulfurtransferase TusC, at 5,356,494 to 5,356,853
_RS25895
BWI76_RS25900: BWI76_RS25900 - sulfurtransferase TusD, at 5,356,853 to 5,357,239
_RS25900
BWI76_RS25905: BWI76_RS25905 - hypothetical protein, at 5,357,239 to 5,357,961
_RS25905
BWI76_RS25910: BWI76_RS25910 - peptidyl-prolyl cis-trans isomerase, at 5,358,128 to 5,358,946
_RS25910
Position (kb) 5357
5358 Strain fitness (log2 ratio) -1
0
1 at 5357.275 kb on - strand at 5357.869 kb on - strand, within BWI76_RS25905 at 5358.166 kb on - strand at 5358.387 kb on + strand, within BWI76_RS25910 at 5358.434 kb on - strand, within BWI76_RS25910 at 5358.507 kb on + strand, within BWI76_RS25910 at 5358.607 kb on - strand, within BWI76_RS25910 at 5358.886 kb on - strand at 5358.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,357,275 - -0.1 5,357,869 - BWI76_RS25905 0.87 -1.2 5,358,166 - +0.5 5,358,387 + BWI76_RS25910 0.32 -0.7 5,358,434 - BWI76_RS25910 0.37 -1.0 5,358,507 + BWI76_RS25910 0.46 -0.6 5,358,607 - BWI76_RS25910 0.58 -0.2 5,358,886 - +1.2 5,358,953 + -1.1
Or see this region's nucleotide sequence