Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25355 and BWI76_RS25360 are separated by 190 nucleotides BWI76_RS25360 and BWI76_RS25365 are separated by 172 nucleotides BWI76_RS25365 and BWI76_RS25370 are separated by 86 nucleotides
BWI76_RS25355: BWI76_RS25355 - cell division protein ZapE, at 5,270,034 to 5,271,167
_RS25355
BWI76_RS25360: BWI76_RS25360 - hypothetical protein, at 5,271,358 to 5,271,756
_RS25360
BWI76_RS25365: BWI76_RS25365 - serine endoprotease DegQ, at 5,271,929 to 5,273,296
_RS25365
BWI76_RS25370: BWI76_RS25370 - outer membrane-stress sensor serine endopeptidase DegS, at 5,273,383 to 5,274,441
_RS25370
Position (kb) 5271
5272
5273
5274 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5270.942 kb on - strand, within BWI76_RS25355 at 5270.942 kb on - strand, within BWI76_RS25355 at 5271.148 kb on + strand at 5271.200 kb on - strand at 5271.230 kb on + strand at 5271.238 kb on - strand at 5271.811 kb on + strand at 5271.853 kb on - strand at 5271.916 kb on - strand at 5271.962 kb on + strand at 5271.969 kb on + strand at 5272.107 kb on - strand, within BWI76_RS25365 at 5272.203 kb on + strand, within BWI76_RS25365 at 5272.211 kb on - strand, within BWI76_RS25365 at 5272.463 kb on - strand, within BWI76_RS25365 at 5272.463 kb on - strand, within BWI76_RS25365 at 5272.947 kb on + strand, within BWI76_RS25365 at 5272.948 kb on + strand, within BWI76_RS25365 at 5273.146 kb on - strand, within BWI76_RS25365 at 5273.152 kb on + strand, within BWI76_RS25365 at 5273.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,270,942 - BWI76_RS25355 0.80 +0.5 5,270,942 - BWI76_RS25355 0.80 +0.4 5,271,148 + +0.9 5,271,200 - -1.4 5,271,230 + +0.3 5,271,238 - +0.5 5,271,811 + +0.9 5,271,853 - -0.5 5,271,916 - +0.2 5,271,962 + -1.8 5,271,969 + -0.9 5,272,107 - BWI76_RS25365 0.13 +2.1 5,272,203 + BWI76_RS25365 0.20 +1.4 5,272,211 - BWI76_RS25365 0.21 +1.9 5,272,463 - BWI76_RS25365 0.39 +1.5 5,272,463 - BWI76_RS25365 0.39 +0.5 5,272,947 + BWI76_RS25365 0.74 +1.1 5,272,948 + BWI76_RS25365 0.74 +1.2 5,273,146 - BWI76_RS25365 0.89 +0.3 5,273,152 + BWI76_RS25365 0.89 +1.1 5,273,341 + -0.8
Or see this region's nucleotide sequence