Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24630 and BWI76_RS24635 are separated by 74 nucleotides BWI76_RS24635 and BWI76_RS24640 overlap by 4 nucleotides BWI76_RS24640 and BWI76_RS24645 are separated by 11 nucleotides
BWI76_RS24630: BWI76_RS24630 - putrescine aminotransferase, at 5,133,326 to 5,134,732
_RS24630
BWI76_RS24635: BWI76_RS24635 - autoinducer-2 (AI-2) modifying protein LsrG, at 5,134,807 to 5,135,103
_RS24635
BWI76_RS24640: BWI76_RS24640 - aldolase, at 5,135,100 to 5,135,987
_RS24640
BWI76_RS24645: BWI76_RS24645 - autoinducer 2 ABC transporter substrate-binding protein, at 5,135,999 to 5,137,000
_RS24645
Position (kb) 5134
5135
5136 Strain fitness (log2 ratio) -1
0
1 at 5133.914 kb on + strand, within BWI76_RS24630 at 5134.078 kb on - strand, within BWI76_RS24630 at 5134.153 kb on + strand, within BWI76_RS24630 at 5134.369 kb on - strand, within BWI76_RS24630 at 5134.415 kb on - strand, within BWI76_RS24630 at 5134.482 kb on + strand, within BWI76_RS24630 at 5134.561 kb on - strand, within BWI76_RS24630 at 5134.786 kb on + strand at 5134.804 kb on + strand at 5134.804 kb on + strand at 5134.812 kb on - strand at 5134.812 kb on - strand at 5134.875 kb on + strand, within BWI76_RS24635 at 5134.909 kb on - strand, within BWI76_RS24635 at 5135.021 kb on + strand, within BWI76_RS24635 at 5135.041 kb on - strand, within BWI76_RS24635 at 5135.136 kb on - strand at 5135.285 kb on - strand, within BWI76_RS24640 at 5135.330 kb on - strand, within BWI76_RS24640 at 5135.490 kb on - strand, within BWI76_RS24640 at 5135.496 kb on - strand, within BWI76_RS24640 at 5135.557 kb on + strand, within BWI76_RS24640 at 5135.565 kb on - strand, within BWI76_RS24640 at 5135.722 kb on - strand, within BWI76_RS24640 at 5135.754 kb on - strand, within BWI76_RS24640 at 5135.938 kb on - strand at 5135.948 kb on - strand at 5136.097 kb on - strand at 5136.101 kb on + strand, within BWI76_RS24645
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 5,133,914 + BWI76_RS24630 0.42 -0.3 5,134,078 - BWI76_RS24630 0.53 -0.1 5,134,153 + BWI76_RS24630 0.59 +0.0 5,134,369 - BWI76_RS24630 0.74 +1.0 5,134,415 - BWI76_RS24630 0.77 +1.4 5,134,482 + BWI76_RS24630 0.82 -1.4 5,134,561 - BWI76_RS24630 0.88 +0.1 5,134,786 + -0.2 5,134,804 + +0.2 5,134,804 + +0.2 5,134,812 - -0.4 5,134,812 - +1.4 5,134,875 + BWI76_RS24635 0.23 -1.4 5,134,909 - BWI76_RS24635 0.34 -1.7 5,135,021 + BWI76_RS24635 0.72 -1.0 5,135,041 - BWI76_RS24635 0.79 -1.4 5,135,136 - -0.7 5,135,285 - BWI76_RS24640 0.21 +0.7 5,135,330 - BWI76_RS24640 0.26 -0.0 5,135,490 - BWI76_RS24640 0.44 -0.2 5,135,496 - BWI76_RS24640 0.45 -0.7 5,135,557 + BWI76_RS24640 0.51 -0.3 5,135,565 - BWI76_RS24640 0.52 +0.1 5,135,722 - BWI76_RS24640 0.70 +0.1 5,135,754 - BWI76_RS24640 0.74 -0.1 5,135,938 - +0.2 5,135,948 - +0.9 5,136,097 - +0.2 5,136,101 + BWI76_RS24645 0.10 +0.2
Or see this region's nucleotide sequence