Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23800 and BWI76_RS23805 are separated by 155 nucleotides BWI76_RS23805 and BWI76_RS23810 are separated by 5 nucleotides BWI76_RS23810 and BWI76_RS23815 are separated by 22 nucleotides
BWI76_RS23800: BWI76_RS23800 - peptide chain release factor 2, at 4,968,113 to 4,969,141
_RS23800
BWI76_RS23805: BWI76_RS23805 - ssDNA exonuclease RecJ, at 4,969,297 to 4,971,030
_RS23805
BWI76_RS23810: BWI76_RS23810 - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 4,971,036 to 4,971,749
_RS23810
BWI76_RS23815: BWI76_RS23815 - site-specific tyrosine recombinase XerD, at 4,971,772 to 4,972,668
_RS23815
Position (kb)
4969
4970
4971
4972 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4969.293 kb on + strand at 4969.563 kb on + strand, within BWI76_RS23805 at 4969.772 kb on + strand, within BWI76_RS23805 at 4969.831 kb on + strand, within BWI76_RS23805 at 4969.846 kb on - strand, within BWI76_RS23805 at 4970.012 kb on - strand, within BWI76_RS23805 at 4970.039 kb on - strand, within BWI76_RS23805 at 4970.088 kb on - strand, within BWI76_RS23805 at 4970.259 kb on - strand, within BWI76_RS23805 at 4970.285 kb on - strand, within BWI76_RS23805 at 4970.504 kb on - strand, within BWI76_RS23805 at 4970.575 kb on - strand, within BWI76_RS23805 at 4970.635 kb on - strand, within BWI76_RS23805 at 4970.820 kb on - strand, within BWI76_RS23805 at 4970.866 kb on + strand at 4970.879 kb on - strand at 4970.879 kb on - strand at 4971.158 kb on + strand, within BWI76_RS23810 at 4971.385 kb on - strand, within BWI76_RS23810 at 4971.469 kb on + strand at 4971.702 kb on + strand at 4971.711 kb on - strand at 4971.838 kb on - strand at 4971.856 kb on + strand at 4971.972 kb on + strand, within BWI76_RS23815
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,969,293 + -0.2 4,969,563 + BWI76_RS23805 0.15 -0.7 4,969,772 + BWI76_RS23805 0.27 -2.9 4,969,831 + BWI76_RS23805 0.31 +0.2 4,969,846 - BWI76_RS23805 0.32 -0.3 4,970,012 - BWI76_RS23805 0.41 -0.3 4,970,039 - BWI76_RS23805 0.43 -1.9 4,970,088 - BWI76_RS23805 0.46 -0.4 4,970,259 - BWI76_RS23805 0.55 -0.8 4,970,285 - BWI76_RS23805 0.57 +0.9 4,970,504 - BWI76_RS23805 0.70 -2.0 4,970,575 - BWI76_RS23805 0.74 +0.3 4,970,635 - BWI76_RS23805 0.77 -0.4 4,970,820 - BWI76_RS23805 0.88 +0.2 4,970,866 + -1.7 4,970,879 - +0.3 4,970,879 - +0.8 4,971,158 + BWI76_RS23810 0.17 -1.4 4,971,385 - BWI76_RS23810 0.49 +0.7 4,971,469 + -0.6 4,971,702 + -0.2 4,971,711 - -2.2 4,971,838 - +1.0 4,971,856 + -0.3 4,971,972 + BWI76_RS23815 0.22 -0.0
Or see this region's nucleotide sequence