Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23560 and BWI76_RS23565 are separated by 35 nucleotides BWI76_RS23565 and BWI76_RS23570 are separated by 73 nucleotides BWI76_RS23570 and BWI76_RS23575 are separated by 10 nucleotides BWI76_RS23575 and BWI76_RS23580 are separated by 12 nucleotides
BWI76_RS23560: BWI76_RS23560 - hypothetical protein, at 4,913,614 to 4,914,153
_RS23560
BWI76_RS23565: BWI76_RS23565 - molecular chaperone FimC, at 4,914,189 to 4,914,914
_RS23565
BWI76_RS23570: BWI76_RS23570 - fimbrial protein, at 4,914,988 to 4,917,624
_RS23570
BWI76_RS23575: BWI76_RS23575 - hypothetical protein, at 4,917,635 to 4,918,165
_RS23575
BWI76_RS23580: BWI76_RS23580 - hypothetical protein, at 4,918,178 to 4,918,678
_RS23580
Position (kb)
4914
4915
4916
4917
4918 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4914.083 kb on + strand, within BWI76_RS23560 at 4914.292 kb on + strand, within BWI76_RS23565 at 4914.421 kb on + strand, within BWI76_RS23565 at 4914.456 kb on - strand, within BWI76_RS23565 at 4914.712 kb on + strand, within BWI76_RS23565 at 4914.712 kb on + strand, within BWI76_RS23565 at 4914.777 kb on - strand, within BWI76_RS23565 at 4914.795 kb on - strand, within BWI76_RS23565 at 4914.815 kb on - strand, within BWI76_RS23565 at 4914.815 kb on - strand, within BWI76_RS23565 at 4914.825 kb on - strand, within BWI76_RS23565 at 4915.025 kb on - strand at 4915.112 kb on - strand at 4915.125 kb on + strand at 4915.396 kb on + strand, within BWI76_RS23570 at 4916.306 kb on + strand, within BWI76_RS23570 at 4916.316 kb on + strand, within BWI76_RS23570 at 4916.570 kb on + strand, within BWI76_RS23570 at 4916.632 kb on + strand, within BWI76_RS23570 at 4917.120 kb on - strand, within BWI76_RS23570 at 4917.155 kb on - strand, within BWI76_RS23570 at 4917.231 kb on - strand, within BWI76_RS23570 at 4917.280 kb on - strand, within BWI76_RS23570 at 4917.320 kb on - strand, within BWI76_RS23570 at 4917.325 kb on - strand, within BWI76_RS23570 at 4917.325 kb on - strand, within BWI76_RS23570 at 4917.329 kb on + strand, within BWI76_RS23570 at 4917.483 kb on - strand at 4917.508 kb on + strand at 4917.656 kb on + strand at 4917.682 kb on - strand at 4918.012 kb on + strand, within BWI76_RS23575 at 4918.053 kb on + strand, within BWI76_RS23575 at 4918.063 kb on + strand at 4918.088 kb on + strand, within BWI76_RS23575 at 4918.196 kb on - strand at 4918.204 kb on - strand at 4918.346 kb on - strand, within BWI76_RS23580 at 4918.346 kb on - strand, within BWI76_RS23580 at 4918.463 kb on - strand, within BWI76_RS23580 at 4918.492 kb on + strand, within BWI76_RS23580
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,914,083 + BWI76_RS23560 0.87 -0.4 4,914,292 + BWI76_RS23565 0.14 +0.3 4,914,421 + BWI76_RS23565 0.32 -0.6 4,914,456 - BWI76_RS23565 0.37 +0.1 4,914,712 + BWI76_RS23565 0.72 -2.1 4,914,712 + BWI76_RS23565 0.72 +0.7 4,914,777 - BWI76_RS23565 0.81 -0.3 4,914,795 - BWI76_RS23565 0.83 -1.3 4,914,815 - BWI76_RS23565 0.86 +0.7 4,914,815 - BWI76_RS23565 0.86 -0.6 4,914,825 - BWI76_RS23565 0.88 -0.1 4,915,025 - +1.0 4,915,112 - +0.0 4,915,125 + +0.1 4,915,396 + BWI76_RS23570 0.15 -0.8 4,916,306 + BWI76_RS23570 0.50 -0.4 4,916,316 + BWI76_RS23570 0.50 -0.3 4,916,570 + BWI76_RS23570 0.60 -2.3 4,916,632 + BWI76_RS23570 0.62 +2.1 4,917,120 - BWI76_RS23570 0.81 +1.1 4,917,155 - BWI76_RS23570 0.82 +0.1 4,917,231 - BWI76_RS23570 0.85 +0.3 4,917,280 - BWI76_RS23570 0.87 -0.9 4,917,320 - BWI76_RS23570 0.88 +0.5 4,917,325 - BWI76_RS23570 0.89 -1.0 4,917,325 - BWI76_RS23570 0.89 -0.8 4,917,329 + BWI76_RS23570 0.89 -2.0 4,917,483 - -0.5 4,917,508 + -0.4 4,917,656 + -1.1 4,917,682 - -0.1 4,918,012 + BWI76_RS23575 0.71 +0.0 4,918,053 + BWI76_RS23575 0.79 -0.2 4,918,063 + -1.4 4,918,088 + BWI76_RS23575 0.85 +0.0 4,918,196 - +1.3 4,918,204 - +0.8 4,918,346 - BWI76_RS23580 0.34 -1.0 4,918,346 - BWI76_RS23580 0.34 +0.2 4,918,463 - BWI76_RS23580 0.57 +1.6 4,918,492 + BWI76_RS23580 0.63 -0.5
Or see this region's nucleotide sequence