Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20930 and BWI76_RS20935 are separated by 28 nucleotides BWI76_RS20935 and BWI76_RS20940 are separated by 71 nucleotides BWI76_RS20940 and BWI76_RS20945 are separated by 95 nucleotides BWI76_RS20945 and BWI76_RS20950 overlap by 63 nucleotides BWI76_RS20950 and BWI76_RS20955 are separated by 54 nucleotides
BWI76_RS20930: BWI76_RS20930 - arsenate reductase (glutaredoxin), at 4,376,392 to 4,376,751
_RS20930
BWI76_RS20935: BWI76_RS20935 - DnaA regulatory inactivator Hda, at 4,376,780 to 4,377,505
_RS20935
BWI76_RS20940: BWI76_RS20940 - uracil/xanthine transporter, at 4,377,577 to 4,378,866
_RS20940
BWI76_RS20945: BWI76_RS20945 - uracil phosphoribosyltransferase, at 4,378,962 to 4,379,588
_RS20945
BWI76_RS20950: BWI76_RS20950 - hypothetical protein, at 4,379,526 to 4,379,716
_RS20950
BWI76_RS20955: BWI76_RS20955 - 6-phospho-beta-glucosidase, at 4,379,771 to 4,381,201
_RS20955
Position (kb)
4377
4378
4379 Strain fitness (log2 ratio)
-2
-1
0
1 at 4376.761 kb on + strand at 4377.517 kb on + strand at 4377.548 kb on - strand at 4377.568 kb on + strand at 4377.576 kb on - strand at 4377.703 kb on - strand at 4377.768 kb on - strand, within BWI76_RS20940 at 4378.272 kb on + strand, within BWI76_RS20940 at 4378.730 kb on + strand, within BWI76_RS20940 at 4378.758 kb on - strand at 4379.050 kb on + strand, within BWI76_RS20945 at 4379.060 kb on - strand, within BWI76_RS20945 at 4379.261 kb on + strand, within BWI76_RS20945 at 4379.477 kb on - strand, within BWI76_RS20945 at 4379.701 kb on + strand at 4379.701 kb on + strand at 4379.709 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,376,761 + -0.2 4,377,517 + -1.4 4,377,548 - +0.3 4,377,568 + -0.3 4,377,576 - -0.9 4,377,703 - -0.2 4,377,768 - BWI76_RS20940 0.15 -0.5 4,378,272 + BWI76_RS20940 0.54 -1.1 4,378,730 + BWI76_RS20940 0.89 -1.8 4,378,758 - +1.7 4,379,050 + BWI76_RS20945 0.14 -1.1 4,379,060 - BWI76_RS20945 0.16 -0.8 4,379,261 + BWI76_RS20945 0.48 +0.6 4,379,477 - BWI76_RS20945 0.82 +0.3 4,379,701 + -0.2 4,379,701 + -0.1 4,379,709 - +0.3
Or see this region's nucleotide sequence