Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19275 and BWI76_RS19280 are separated by 292 nucleotides BWI76_RS19280 and BWI76_RS19285 are separated by 244 nucleotides
BWI76_RS19275: BWI76_RS19275 - DNA-binding response regulator, at 4,015,488 to 4,016,210
_RS19275
BWI76_RS19280: BWI76_RS19280 - U32 family peptidase, at 4,016,503 to 4,017,864
_RS19280
BWI76_RS19285: BWI76_RS19285 - lipid kinase YegS, at 4,018,109 to 4,019,002
_RS19285
Position (kb)
4016
4017
4018 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 4015.803 kb on + strand, within BWI76_RS19275 at 4015.817 kb on + strand, within BWI76_RS19275 at 4015.867 kb on + strand, within BWI76_RS19275 at 4016.167 kb on + strand at 4016.168 kb on - strand at 4016.435 kb on - strand at 4016.454 kb on - strand at 4016.481 kb on - strand at 4016.581 kb on + strand at 4016.685 kb on - strand, within BWI76_RS19280 at 4016.720 kb on - strand, within BWI76_RS19280 at 4016.856 kb on + strand, within BWI76_RS19280 at 4016.856 kb on + strand, within BWI76_RS19280 at 4017.009 kb on + strand, within BWI76_RS19280 at 4017.553 kb on + strand, within BWI76_RS19280 at 4017.730 kb on + strand at 4017.767 kb on + strand at 4017.840 kb on - strand at 4017.994 kb on + strand at 4018.002 kb on - strand at 4018.073 kb on + strand at 4018.325 kb on - strand, within BWI76_RS19285 at 4018.446 kb on + strand, within BWI76_RS19285 at 4018.494 kb on - strand, within BWI76_RS19285 at 4018.631 kb on - strand, within BWI76_RS19285 at 4018.687 kb on - strand, within BWI76_RS19285 at 4018.797 kb on + strand, within BWI76_RS19285 at 4018.797 kb on + strand, within BWI76_RS19285 at 4018.805 kb on - strand, within BWI76_RS19285 at 4018.805 kb on - strand, within BWI76_RS19285 at 4018.805 kb on - strand, within BWI76_RS19285 at 4018.815 kb on - strand, within BWI76_RS19285 at 4018.815 kb on - strand, within BWI76_RS19285
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,015,803 + BWI76_RS19275 0.44 +3.6 4,015,817 + BWI76_RS19275 0.46 +0.0 4,015,867 + BWI76_RS19275 0.52 +0.5 4,016,167 + +0.9 4,016,168 - +2.2 4,016,435 - -0.3 4,016,454 - -0.2 4,016,481 - -1.4 4,016,581 + +3.1 4,016,685 - BWI76_RS19280 0.13 -0.2 4,016,720 - BWI76_RS19280 0.16 -1.7 4,016,856 + BWI76_RS19280 0.26 -1.2 4,016,856 + BWI76_RS19280 0.26 -1.2 4,017,009 + BWI76_RS19280 0.37 +0.9 4,017,553 + BWI76_RS19280 0.77 -1.6 4,017,730 + +0.0 4,017,767 + +1.4 4,017,840 - +0.1 4,017,994 + -0.1 4,018,002 - +0.3 4,018,073 + +0.5 4,018,325 - BWI76_RS19285 0.24 +0.8 4,018,446 + BWI76_RS19285 0.38 +0.1 4,018,494 - BWI76_RS19285 0.43 -1.0 4,018,631 - BWI76_RS19285 0.58 +4.1 4,018,687 - BWI76_RS19285 0.65 +0.2 4,018,797 + BWI76_RS19285 0.77 -0.7 4,018,797 + BWI76_RS19285 0.77 -0.6 4,018,805 - BWI76_RS19285 0.78 +0.7 4,018,805 - BWI76_RS19285 0.78 +1.2 4,018,805 - BWI76_RS19285 0.78 -1.1 4,018,815 - BWI76_RS19285 0.79 -0.2 4,018,815 - BWI76_RS19285 0.79 +1.0
Or see this region's nucleotide sequence