Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17270 and BWI76_RS17275 are separated by 197 nucleotides BWI76_RS17275 and BWI76_RS17280 are separated by 89 nucleotides
BWI76_RS17270: BWI76_RS17270 - UTP--glucose-1-phosphate uridylyltransferase, at 3,578,744 to 3,579,646
_RS17270
BWI76_RS17275: BWI76_RS17275 - two-component system response regulator RssB, at 3,579,844 to 3,580,857
_RS17275
BWI76_RS17280: BWI76_RS17280 - patatin family protein, at 3,580,947 to 3,581,846
_RS17280
Position (kb)
3579
3580
3581 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3579.564 kb on + strand at 3579.661 kb on - strand at 3579.661 kb on - strand at 3579.661 kb on - strand at 3579.704 kb on + strand at 3579.708 kb on - strand at 3579.712 kb on - strand at 3579.731 kb on - strand at 3579.795 kb on + strand at 3579.816 kb on - strand at 3579.816 kb on - strand at 3579.847 kb on - strand at 3579.859 kb on + strand at 3579.908 kb on - strand at 3579.931 kb on - strand at 3579.992 kb on + strand, within BWI76_RS17275 at 3579.992 kb on + strand, within BWI76_RS17275 at 3580.000 kb on - strand at 3580.000 kb on - strand, within BWI76_RS17275 at 3580.000 kb on - strand, within BWI76_RS17275 at 3580.022 kb on - strand, within BWI76_RS17275 at 3580.061 kb on - strand, within BWI76_RS17275 at 3580.088 kb on + strand, within BWI76_RS17275 at 3580.096 kb on - strand, within BWI76_RS17275 at 3580.117 kb on - strand, within BWI76_RS17275 at 3580.140 kb on - strand, within BWI76_RS17275 at 3580.142 kb on + strand, within BWI76_RS17275 at 3580.142 kb on + strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.219 kb on - strand, within BWI76_RS17275 at 3580.299 kb on - strand, within BWI76_RS17275 at 3580.301 kb on + strand, within BWI76_RS17275 at 3580.373 kb on + strand, within BWI76_RS17275 at 3580.383 kb on + strand, within BWI76_RS17275 at 3580.400 kb on - strand, within BWI76_RS17275 at 3580.400 kb on - strand, within BWI76_RS17275 at 3580.569 kb on + strand, within BWI76_RS17275 at 3580.786 kb on + strand at 3580.794 kb on - strand at 3580.794 kb on - strand at 3580.868 kb on - strand at 3580.868 kb on - strand at 3580.898 kb on + strand at 3580.904 kb on + strand at 3580.949 kb on + strand at 3580.949 kb on + strand at 3580.957 kb on - strand at 3580.977 kb on - strand at 3581.020 kb on + strand at 3581.114 kb on - strand, within BWI76_RS17280 at 3581.423 kb on + strand, within BWI76_RS17280 at 3581.423 kb on + strand, within BWI76_RS17280 at 3581.581 kb on - strand, within BWI76_RS17280
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,579,564 + +0.7 3,579,661 - -0.0 3,579,661 - -1.0 3,579,661 - -2.5 3,579,704 + -0.5 3,579,708 - +0.9 3,579,712 - +0.6 3,579,731 - +0.3 3,579,795 + -1.5 3,579,816 - +0.3 3,579,816 - -0.2 3,579,847 - +0.2 3,579,859 + -0.6 3,579,908 - -0.7 3,579,931 - +1.8 3,579,992 + BWI76_RS17275 0.15 +0.1 3,579,992 + BWI76_RS17275 0.15 -0.8 3,580,000 - +1.0 3,580,000 - BWI76_RS17275 0.15 -0.9 3,580,000 - BWI76_RS17275 0.15 +1.3 3,580,022 - BWI76_RS17275 0.18 +0.4 3,580,061 - BWI76_RS17275 0.21 +1.5 3,580,088 + BWI76_RS17275 0.24 +0.4 3,580,096 - BWI76_RS17275 0.25 -1.5 3,580,117 - BWI76_RS17275 0.27 +0.5 3,580,140 - BWI76_RS17275 0.29 -2.3 3,580,142 + BWI76_RS17275 0.29 -1.1 3,580,142 + BWI76_RS17275 0.29 +0.8 3,580,150 - BWI76_RS17275 0.30 +0.2 3,580,150 - BWI76_RS17275 0.30 +0.9 3,580,150 - BWI76_RS17275 0.30 +0.5 3,580,150 - BWI76_RS17275 0.30 -0.2 3,580,184 + BWI76_RS17275 0.34 +1.5 3,580,184 + BWI76_RS17275 0.34 -0.0 3,580,184 + BWI76_RS17275 0.34 +0.0 3,580,184 + BWI76_RS17275 0.34 -1.0 3,580,192 - BWI76_RS17275 0.34 -0.8 3,580,192 - BWI76_RS17275 0.34 +0.3 3,580,192 - BWI76_RS17275 0.34 +0.1 3,580,219 - BWI76_RS17275 0.37 -0.3 3,580,299 - BWI76_RS17275 0.45 -1.2 3,580,301 + BWI76_RS17275 0.45 -1.2 3,580,373 + BWI76_RS17275 0.52 -0.9 3,580,383 + BWI76_RS17275 0.53 +1.8 3,580,400 - BWI76_RS17275 0.55 +0.7 3,580,400 - BWI76_RS17275 0.55 +1.2 3,580,569 + BWI76_RS17275 0.71 +0.0 3,580,786 + -0.2 3,580,794 - +1.6 3,580,794 - -1.6 3,580,868 - -0.1 3,580,868 - +0.6 3,580,898 + +1.4 3,580,904 + -2.2 3,580,949 + +1.1 3,580,949 + +0.6 3,580,957 - +0.8 3,580,977 - +0.3 3,581,020 + -0.3 3,581,114 - BWI76_RS17280 0.19 -2.7 3,581,423 + BWI76_RS17280 0.53 -0.4 3,581,423 + BWI76_RS17280 0.53 -0.6 3,581,581 - BWI76_RS17280 0.70 -1.8
Or see this region's nucleotide sequence