Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15035

Experiment: Acanthamoeba sp., muxed culture-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15025 and BWI76_RS15030 overlap by 4 nucleotidesBWI76_RS15030 and BWI76_RS15035 overlap by 16 nucleotidesBWI76_RS15035 and BWI76_RS15040 are separated by 31 nucleotides BWI76_RS15025: BWI76_RS15025 - NLPA lipoprotein, at 3,145,653 to 3,146,459 _RS15025 BWI76_RS15030: BWI76_RS15030 - ABC transporter, at 3,146,456 to 3,147,247 _RS15030 BWI76_RS15035: BWI76_RS15035 - metal ABC transporter permease, at 3,147,232 to 3,147,903 _RS15035 BWI76_RS15040: BWI76_RS15040 - putative 2-nitropropane dioxygenase, at 3,147,935 to 3,148,981 _RS15040 Position (kb) 3147 3148Strain fitness (log2 ratio) -1 0 1 2 3at 3146.294 kb on - strand, within BWI76_RS15025at 3146.888 kb on - strand, within BWI76_RS15030at 3147.155 kb on - strand, within BWI76_RS15030at 3147.407 kb on - strand, within BWI76_RS15035at 3147.752 kb on + strand, within BWI76_RS15035at 3148.203 kb on + strand, within BWI76_RS15040at 3148.263 kb on + strand, within BWI76_RS15040at 3148.302 kb on - strand, within BWI76_RS15040at 3148.304 kb on + strand, within BWI76_RS15040at 3148.304 kb on + strand, within BWI76_RS15040at 3148.317 kb on + strand, within BWI76_RS15040at 3148.389 kb on - strand, within BWI76_RS15040at 3148.478 kb on - strand, within BWI76_RS15040at 3148.509 kb on - strand, within BWI76_RS15040at 3148.603 kb on + strand, within BWI76_RS15040

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Per-strain Table

Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2
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3,146,294 - BWI76_RS15025 0.79 -0.4
3,146,888 - BWI76_RS15030 0.55 -0.4
3,147,155 - BWI76_RS15030 0.88 +0.2
3,147,407 - BWI76_RS15035 0.26 -1.6
3,147,752 + BWI76_RS15035 0.77 +0.2
3,148,203 + BWI76_RS15040 0.26 +0.1
3,148,263 + BWI76_RS15040 0.31 -1.2
3,148,302 - BWI76_RS15040 0.35 +0.0
3,148,304 + BWI76_RS15040 0.35 +3.1
3,148,304 + BWI76_RS15040 0.35 -0.8
3,148,317 + BWI76_RS15040 0.36 +0.4
3,148,389 - BWI76_RS15040 0.43 -1.3
3,148,478 - BWI76_RS15040 0.52 +0.3
3,148,509 - BWI76_RS15040 0.55 +0.6
3,148,603 + BWI76_RS15040 0.64 +0.7

Or see this region's nucleotide sequence