Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14835 and BWI76_RS14840 are separated by 180 nucleotides BWI76_RS14840 and BWI76_RS14845 are separated by 208 nucleotides BWI76_RS14845 and BWI76_RS14850 are separated by 366 nucleotides
BWI76_RS14835: BWI76_RS14835 - hypothetical protein, at 3,108,038 to 3,108,430
_RS14835
BWI76_RS14840: BWI76_RS14840 - hypothetical protein, at 3,108,611 to 3,109,213
_RS14840
BWI76_RS14845: BWI76_RS14845 - hypothetical protein, at 3,109,422 to 3,110,048
_RS14845
BWI76_RS14850: BWI76_RS14850 - LacI family transcriptional regulator, at 3,110,415 to 3,111,446
_RS14850
Position (kb)
3109
3110
3111 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3108.425 kb on + strand at 3108.594 kb on - strand at 3108.726 kb on - strand, within BWI76_RS14840 at 3108.847 kb on - strand, within BWI76_RS14840 at 3109.135 kb on + strand, within BWI76_RS14840 at 3109.175 kb on - strand at 3109.240 kb on + strand at 3109.361 kb on - strand at 3109.674 kb on - strand, within BWI76_RS14845 at 3109.679 kb on - strand, within BWI76_RS14845 at 3109.803 kb on - strand, within BWI76_RS14845 at 3110.173 kb on - strand at 3110.219 kb on + strand at 3110.219 kb on + strand at 3110.219 kb on + strand at 3110.826 kb on - strand, within BWI76_RS14850 at 3110.991 kb on - strand, within BWI76_RS14850 at 3111.013 kb on - strand, within BWI76_RS14850
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 3,108,425 + +0.2 3,108,594 - -0.4 3,108,726 - BWI76_RS14840 0.19 +0.5 3,108,847 - BWI76_RS14840 0.39 +1.1 3,109,135 + BWI76_RS14840 0.87 -0.3 3,109,175 - -0.0 3,109,240 + +1.1 3,109,361 - +0.0 3,109,674 - BWI76_RS14845 0.40 +3.2 3,109,679 - BWI76_RS14845 0.41 -0.4 3,109,803 - BWI76_RS14845 0.61 +0.8 3,110,173 - -0.5 3,110,219 + -0.1 3,110,219 + -0.0 3,110,219 + -0.5 3,110,826 - BWI76_RS14850 0.40 +1.0 3,110,991 - BWI76_RS14850 0.56 +1.0 3,111,013 - BWI76_RS14850 0.58 -0.4
Or see this region's nucleotide sequence