Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13430 and BWI76_RS13435 are separated by 52 nucleotides BWI76_RS13435 and BWI76_RS13440 are separated by 196 nucleotides BWI76_RS13440 and BWI76_RS13445 are separated by 72 nucleotides
BWI76_RS13430: BWI76_RS13430 - hypothetical protein, at 2,802,498 to 2,802,782
_RS13430
BWI76_RS13435: BWI76_RS13435 - hypothetical protein, at 2,802,835 to 2,802,927
_RS13435
BWI76_RS13440: BWI76_RS13440 - DUF2566 domain-containing protein, at 2,803,124 to 2,803,297
_RS13440
BWI76_RS13445: BWI76_RS13445 - alpha/beta hydrolase, at 2,803,370 to 2,804,140
_RS13445
Position (kb)
2802
2803 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2801.855 kb on - strand at 2802.065 kb on - strand at 2802.194 kb on - strand at 2802.210 kb on + strand at 2802.210 kb on + strand at 2802.210 kb on + strand at 2802.210 kb on + strand at 2802.210 kb on + strand at 2802.218 kb on - strand at 2802.218 kb on - strand at 2802.218 kb on - strand at 2802.218 kb on - strand at 2802.232 kb on + strand at 2802.245 kb on + strand at 2802.245 kb on + strand at 2802.245 kb on + strand at 2802.253 kb on - strand at 2802.260 kb on - strand at 2802.265 kb on + strand at 2802.265 kb on + strand at 2802.273 kb on - strand at 2802.405 kb on + strand at 2802.406 kb on - strand at 2802.406 kb on - strand at 2802.455 kb on - strand at 2802.470 kb on - strand at 2802.482 kb on - strand at 2802.658 kb on + strand, within BWI76_RS13430 at 2802.898 kb on - strand, within BWI76_RS13435 at 2802.957 kb on + strand at 2802.968 kb on + strand at 2803.061 kb on + strand at 2803.388 kb on + strand at 2803.593 kb on - strand, within BWI76_RS13445 at 2803.782 kb on - strand, within BWI76_RS13445
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,801,855 - -0.2 2,802,065 - -0.2 2,802,194 - -0.8 2,802,210 + +1.6 2,802,210 + +2.4 2,802,210 + -1.0 2,802,210 + -0.5 2,802,210 + -0.9 2,802,218 - +0.8 2,802,218 - +0.0 2,802,218 - +1.7 2,802,218 - -1.3 2,802,232 + -1.0 2,802,245 + +0.3 2,802,245 + +0.0 2,802,245 + -0.2 2,802,253 - -0.5 2,802,260 - -1.4 2,802,265 + -0.3 2,802,265 + +0.2 2,802,273 - +1.0 2,802,405 + +0.3 2,802,406 - -1.1 2,802,406 - -0.9 2,802,455 - +0.2 2,802,470 - +0.1 2,802,482 - -0.3 2,802,658 + BWI76_RS13430 0.56 -0.8 2,802,898 - BWI76_RS13435 0.68 -2.1 2,802,957 + -2.9 2,802,968 + +1.9 2,803,061 + -0.5 2,803,388 + -1.9 2,803,593 - BWI76_RS13445 0.29 +0.4 2,803,782 - BWI76_RS13445 0.53 +0.2
Or see this region's nucleotide sequence