Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotides BWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotides BWI76_RS12875 and BWI76_RS12880 are separated by 72 nucleotides
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861
_RS12870
BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557
_RS12875
BWI76_RS12880: BWI76_RS12880 - aspartate-semialdehyde dehydrogenase, at 2,683,630 to 2,684,748
_RS12880
Position (kb)
2682
2683 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2681.110 kb on - strand, within BWI76_RS12865 at 2681.268 kb on + strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.518 kb on - strand, within BWI76_RS12865 at 2681.906 kb on + strand at 2681.921 kb on - strand at 2682.117 kb on + strand at 2682.117 kb on + strand at 2682.205 kb on - strand, within BWI76_RS12870 at 2682.302 kb on + strand, within BWI76_RS12870 at 2682.418 kb on + strand, within BWI76_RS12870 at 2682.797 kb on + strand at 2682.911 kb on + strand at 2683.019 kb on + strand at 2683.148 kb on - strand, within BWI76_RS12875 at 2683.188 kb on + strand, within BWI76_RS12875 at 2683.225 kb on - strand, within BWI76_RS12875 at 2683.379 kb on - strand, within BWI76_RS12875 at 2683.526 kb on - strand at 2683.568 kb on + strand at 2683.573 kb on - strand at 2683.576 kb on - strand at 2683.612 kb on - strand at 2683.663 kb on + strand at 2683.700 kb on + strand at 2683.713 kb on - strand at 2683.725 kb on + strand at 2683.791 kb on - strand, within BWI76_RS12880 at 2683.801 kb on - strand, within BWI76_RS12880 at 2683.811 kb on - strand, within BWI76_RS12880
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,681,110 - BWI76_RS12865 0.22 -0.1 2,681,268 + BWI76_RS12865 0.35 -1.0 2,681,368 - BWI76_RS12865 0.44 -0.5 2,681,368 - BWI76_RS12865 0.44 +1.6 2,681,518 - BWI76_RS12865 0.57 +2.4 2,681,906 + +0.2 2,681,921 - +1.0 2,682,117 + -0.1 2,682,117 + -0.5 2,682,205 - BWI76_RS12870 0.19 -0.9 2,682,302 + BWI76_RS12870 0.31 -1.5 2,682,418 + BWI76_RS12870 0.46 -0.6 2,682,797 + -1.0 2,682,911 + -0.2 2,683,019 + +4.0 2,683,148 - BWI76_RS12875 0.24 -0.6 2,683,188 + BWI76_RS12875 0.31 -0.8 2,683,225 - BWI76_RS12875 0.38 -0.3 2,683,379 - BWI76_RS12875 0.67 -2.1 2,683,526 - -0.1 2,683,568 + +0.5 2,683,573 - +0.5 2,683,576 - -0.5 2,683,612 - -0.9 2,683,663 + -0.2 2,683,700 + -2.0 2,683,713 - +0.9 2,683,725 + -1.0 2,683,791 - BWI76_RS12880 0.14 -2.1 2,683,801 - BWI76_RS12880 0.15 -0.8 2,683,811 - BWI76_RS12880 0.16 +0.9
Or see this region's nucleotide sequence