Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10645 and BWI76_RS10650 are separated by 125 nucleotides BWI76_RS10650 and BWI76_RS10655 are separated by 15 nucleotides BWI76_RS10655 and BWI76_RS10660 overlap by 35 nucleotides
BWI76_RS10645: BWI76_RS10645 - phytanoyl-CoA dioxygenase, at 2,247,972 to 2,248,739
_RS10645
BWI76_RS10650: BWI76_RS10650 - PTS fructose-like transporter subunit EIIB, at 2,248,865 to 2,249,206
_RS10650
BWI76_RS10655: BWI76_RS10655 - [formate-C-acetyltransferase]-activating enzyme, at 2,249,222 to 2,250,097
_RS10655
BWI76_RS10660: BWI76_RS10660 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 2,250,063 to 2,252,360
_RS10660
Position (kb)
2248
2249
2250 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2247.922 kb on + strand at 2248.058 kb on + strand, within BWI76_RS10645 at 2248.058 kb on + strand, within BWI76_RS10645 at 2248.058 kb on + strand, within BWI76_RS10645 at 2248.071 kb on - strand, within BWI76_RS10645 at 2248.354 kb on + strand, within BWI76_RS10645 at 2248.411 kb on + strand, within BWI76_RS10645 at 2248.419 kb on - strand, within BWI76_RS10645 at 2248.419 kb on - strand, within BWI76_RS10645 at 2248.488 kb on + strand, within BWI76_RS10645 at 2249.028 kb on + strand, within BWI76_RS10650 at 2249.048 kb on - strand, within BWI76_RS10650 at 2249.148 kb on + strand, within BWI76_RS10650 at 2249.150 kb on + strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2250.094 kb on - strand at 2250.097 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,247,922 + -0.6 2,248,058 + BWI76_RS10645 0.11 -0.0 2,248,058 + BWI76_RS10645 0.11 -0.6 2,248,058 + BWI76_RS10645 0.11 -0.3 2,248,071 - BWI76_RS10645 0.13 +1.9 2,248,354 + BWI76_RS10645 0.50 +2.1 2,248,411 + BWI76_RS10645 0.57 -0.5 2,248,419 - BWI76_RS10645 0.58 -0.3 2,248,419 - BWI76_RS10645 0.58 -0.2 2,248,488 + BWI76_RS10645 0.67 -1.1 2,249,028 + BWI76_RS10650 0.48 +0.2 2,249,048 - BWI76_RS10650 0.54 +0.9 2,249,148 + BWI76_RS10650 0.83 +1.4 2,249,150 + BWI76_RS10650 0.83 +1.0 2,249,156 - BWI76_RS10650 0.85 -2.0 2,249,156 - BWI76_RS10650 0.85 -1.4 2,249,389 + BWI76_RS10655 0.19 +2.5 2,249,389 + BWI76_RS10655 0.19 -1.8 2,249,389 + BWI76_RS10655 0.19 +0.6 2,249,397 - BWI76_RS10655 0.20 -0.4 2,249,397 - BWI76_RS10655 0.20 -0.4 2,250,094 - -1.1 2,250,097 + -1.4
Or see this region's nucleotide sequence