Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09950 and BWI76_RS09955 are separated by 65 nucleotides BWI76_RS09955 and BWI76_RS09960 are separated by 69 nucleotides BWI76_RS09960 and BWI76_RS09965 are separated by 185 nucleotides BWI76_RS09965 and BWI76_RS09970 are separated by 95 nucleotides
BWI76_RS09950: BWI76_RS09950 - ribosome modulation factor, at 2,094,910 to 2,095,077
_RS09950
BWI76_RS09955: BWI76_RS09955 - beta-hydroxydecanoyl-ACP dehydratase, at 2,095,143 to 2,095,661
_RS09955
BWI76_RS09960: BWI76_RS09960 - Lon protease, at 2,095,731 to 2,097,488
_RS09960
BWI76_RS09965: BWI76_RS09965 - macrodomain Ter protein, at 2,097,674 to 2,098,126
_RS09965
BWI76_RS09970: BWI76_RS09970 - porin OmpA, at 2,098,222 to 2,099,292
_RS09970
Position (kb)
2095
2096
2097
2098 Strain fitness (log2 ratio)
-2
-1
0
1 at 2095.070 kb on - strand at 2096.165 kb on - strand, within BWI76_RS09960 at 2096.259 kb on - strand, within BWI76_RS09960 at 2097.051 kb on + strand, within BWI76_RS09960 at 2098.178 kb on - strand at 2098.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,095,070 - +0.3 2,096,165 - BWI76_RS09960 0.25 -2.3 2,096,259 - BWI76_RS09960 0.30 +0.1 2,097,051 + BWI76_RS09960 0.75 -1.0 2,098,178 - +0.1 2,098,290 - -0.8
Or see this region's nucleotide sequence