Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06825 and BWI76_RS06830 overlap by 1 nucleotides BWI76_RS06830 and BWI76_RS06835 are separated by 111 nucleotides BWI76_RS06835 and BWI76_RS06840 are separated by 176 nucleotides BWI76_RS06840 and BWI76_RS06845 are separated by 127 nucleotides
BWI76_RS06825: BWI76_RS06825 - nucleoid-associated protein, YbaB/EbfC family, at 1,432,049 to 1,432,381
_RS06825
BWI76_RS06830: BWI76_RS06830 - recombination protein RecR, at 1,432,381 to 1,432,986
_RS06830
BWI76_RS06835: BWI76_RS06835 - molecular chaperone HtpG, at 1,433,098 to 1,434,972
_RS06835
BWI76_RS06840: BWI76_RS06840 - adenylate kinase, at 1,435,149 to 1,435,793
_RS06840
BWI76_RS06845: BWI76_RS06845 - ferrochelatase, at 1,435,921 to 1,436,883
_RS06845
Position (kb)
1433
1434
1435 Strain fitness (log2 ratio)
-1
0
1 at 1432.436 kb on + strand at 1432.636 kb on + strand, within BWI76_RS06830 at 1432.694 kb on + strand, within BWI76_RS06830 at 1432.869 kb on + strand, within BWI76_RS06830 at 1432.874 kb on + strand, within BWI76_RS06830 at 1433.024 kb on + strand at 1433.035 kb on + strand at 1433.213 kb on + strand at 1433.347 kb on + strand, within BWI76_RS06835 at 1433.436 kb on + strand, within BWI76_RS06835 at 1433.436 kb on + strand, within BWI76_RS06835 at 1433.535 kb on - strand, within BWI76_RS06835 at 1433.914 kb on + strand, within BWI76_RS06835 at 1433.928 kb on + strand, within BWI76_RS06835 at 1434.153 kb on + strand, within BWI76_RS06835 at 1434.947 kb on + strand at 1434.985 kb on + strand at 1434.985 kb on + strand at 1434.991 kb on - strand at 1434.993 kb on - strand at 1434.993 kb on - strand at 1434.993 kb on - strand at 1435.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,432,436 + -0.1 1,432,636 + BWI76_RS06830 0.42 -1.1 1,432,694 + BWI76_RS06830 0.52 -0.1 1,432,869 + BWI76_RS06830 0.81 -0.7 1,432,874 + BWI76_RS06830 0.81 +0.4 1,433,024 + -0.1 1,433,035 + -0.5 1,433,213 + -0.7 1,433,347 + BWI76_RS06835 0.13 -1.5 1,433,436 + BWI76_RS06835 0.18 -0.4 1,433,436 + BWI76_RS06835 0.18 -0.4 1,433,535 - BWI76_RS06835 0.23 -0.6 1,433,914 + BWI76_RS06835 0.44 -0.1 1,433,928 + BWI76_RS06835 0.44 -0.1 1,434,153 + BWI76_RS06835 0.56 -0.6 1,434,947 + +0.0 1,434,985 + +0.8 1,434,985 + +0.0 1,434,991 - -1.1 1,434,993 - -0.6 1,434,993 - +1.4 1,434,993 - -0.3 1,435,869 - -1.3
Or see this region's nucleotide sequence