Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06180 and BWI76_RS06185 are separated by 334 nucleotides BWI76_RS06185 and BWI76_RS06190 are separated by 71 nucleotides BWI76_RS06190 and BWI76_RS06195 are separated by 268 nucleotides
BWI76_RS06180: BWI76_RS06180 - hypothetical protein, at 1,297,344 to 1,298,042
_RS06180
BWI76_RS06185: BWI76_RS06185 - shikimate kinase II, at 1,298,377 to 1,298,904
_RS06185
BWI76_RS06190: BWI76_RS06190 - hypothetical protein, at 1,298,976 to 1,299,167
_RS06190
BWI76_RS06195: BWI76_RS06195 - hypothetical protein, at 1,299,436 to 1,300,101
_RS06195
Position (kb)
1298
1299
1300 Strain fitness (log2 ratio)
-1
0
1
2 at 1298.038 kb on - strand at 1298.039 kb on + strand at 1298.052 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.321 kb on - strand at 1298.359 kb on + strand at 1298.367 kb on - strand at 1298.367 kb on - strand at 1298.456 kb on + strand, within BWI76_RS06185 at 1298.464 kb on - strand, within BWI76_RS06185 at 1298.500 kb on - strand, within BWI76_RS06185 at 1298.615 kb on + strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.645 kb on - strand, within BWI76_RS06185 at 1298.672 kb on + strand, within BWI76_RS06185 at 1298.705 kb on - strand, within BWI76_RS06185 at 1298.762 kb on + strand, within BWI76_RS06185 at 1298.805 kb on - strand, within BWI76_RS06185 at 1298.898 kb on - strand at 1299.063 kb on - strand, within BWI76_RS06190 at 1299.063 kb on - strand, within BWI76_RS06190 at 1299.063 kb on - strand, within BWI76_RS06190 at 1299.325 kb on - strand at 1299.477 kb on + strand at 1299.630 kb on + strand, within BWI76_RS06195 at 1299.663 kb on - strand, within BWI76_RS06195 at 1299.665 kb on + strand, within BWI76_RS06195 at 1299.717 kb on + strand, within BWI76_RS06195 at 1299.773 kb on + strand, within BWI76_RS06195 at 1299.809 kb on + strand, within BWI76_RS06195 at 1299.809 kb on + strand, within BWI76_RS06195 at 1299.817 kb on - strand, within BWI76_RS06195 at 1299.891 kb on + strand, within BWI76_RS06195 at 1299.902 kb on + strand, within BWI76_RS06195 at 1300.156 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 1,298,038 - +1.3 1,298,039 + -0.3 1,298,052 - +0.1 1,298,125 - +1.2 1,298,125 - -0.5 1,298,125 - -0.8 1,298,321 - +2.2 1,298,359 + +0.6 1,298,367 - +1.6 1,298,367 - +1.1 1,298,456 + BWI76_RS06185 0.15 -0.6 1,298,464 - BWI76_RS06185 0.16 -0.6 1,298,500 - BWI76_RS06185 0.23 -0.3 1,298,615 + BWI76_RS06185 0.45 -0.2 1,298,623 - BWI76_RS06185 0.47 +2.6 1,298,623 - BWI76_RS06185 0.47 +1.5 1,298,623 - BWI76_RS06185 0.47 -0.7 1,298,623 - BWI76_RS06185 0.47 +0.2 1,298,645 - BWI76_RS06185 0.51 +0.5 1,298,672 + BWI76_RS06185 0.56 -0.1 1,298,705 - BWI76_RS06185 0.62 +0.5 1,298,762 + BWI76_RS06185 0.73 +0.0 1,298,805 - BWI76_RS06185 0.81 +0.7 1,298,898 - +0.4 1,299,063 - BWI76_RS06190 0.45 +0.2 1,299,063 - BWI76_RS06190 0.45 +2.4 1,299,063 - BWI76_RS06190 0.45 -0.5 1,299,325 - -0.1 1,299,477 + -0.3 1,299,630 + BWI76_RS06195 0.29 -1.6 1,299,663 - BWI76_RS06195 0.34 +0.5 1,299,665 + BWI76_RS06195 0.34 +0.0 1,299,717 + BWI76_RS06195 0.42 +1.1 1,299,773 + BWI76_RS06195 0.51 -0.3 1,299,809 + BWI76_RS06195 0.56 -1.7 1,299,809 + BWI76_RS06195 0.56 +0.1 1,299,817 - BWI76_RS06195 0.57 -1.1 1,299,891 + BWI76_RS06195 0.68 -1.0 1,299,902 + BWI76_RS06195 0.70 -1.0 1,300,156 - -0.8
Or see this region's nucleotide sequence