Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04460 and BWI76_RS04465 are separated by 457 nucleotides BWI76_RS04465 and BWI76_RS04470 are separated by 18 nucleotides
BWI76_RS04460: BWI76_RS04460 - 4-hydroxy-tetrahydrodipicolinate reductase, at 921,267 to 922,088
_RS04460
BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694
_RS04465
BWI76_RS04470: BWI76_RS04470 - carbamoyl phosphate synthase large subunit, at 923,713 to 926,937
_RS04470
Position (kb) 922
923
924 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 922.434 kb on + strand at 922.440 kb on - strand at 922.440 kb on - strand at 922.599 kb on + strand at 922.828 kb on + strand, within BWI76_RS04465 at 922.836 kb on + strand, within BWI76_RS04465 at 922.836 kb on - strand, within BWI76_RS04465 at 922.897 kb on - strand, within BWI76_RS04465 at 923.052 kb on - strand, within BWI76_RS04465 at 923.206 kb on + strand, within BWI76_RS04465 at 923.623 kb on - strand at 923.628 kb on - strand at 923.794 kb on - strand at 923.808 kb on + strand at 923.816 kb on - strand at 923.855 kb on + strand at 923.862 kb on + strand at 923.879 kb on - strand at 923.995 kb on + strand at 923.995 kb on + strand at 924.003 kb on - strand at 924.256 kb on + strand, within BWI76_RS04470 at 924.264 kb on - strand at 924.575 kb on - strand, within BWI76_RS04470
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 922,434 + +0.4 922,440 - -1.4 922,440 - +1.9 922,599 + -2.6 922,828 + BWI76_RS04465 0.25 -3.8 922,836 + BWI76_RS04465 0.25 -4.8 922,836 - BWI76_RS04465 0.25 -2.4 922,897 - BWI76_RS04465 0.31 +0.9 923,052 - BWI76_RS04465 0.44 -2.9 923,206 + BWI76_RS04465 0.57 -0.4 923,623 - -0.2 923,628 - -3.6 923,794 - -4.0 923,808 + -2.0 923,816 - +0.9 923,855 + -1.2 923,862 + -4.1 923,879 - -3.1 923,995 + -1.2 923,995 + -0.1 924,003 - -3.8 924,256 + BWI76_RS04470 0.17 -1.9 924,264 - -0.6 924,575 - BWI76_RS04470 0.27 -2.6
Or see this region's nucleotide sequence