Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03420 and BWI76_RS03425 overlap by 14 nucleotides BWI76_RS03425 and BWI76_RS03430 overlap by 7 nucleotides BWI76_RS03430 and BWI76_RS03435 are separated by 23 nucleotides
BWI76_RS03420: BWI76_RS03420 - hypothetical protein, at 704,217 to 704,648
_RS03420
BWI76_RS03425: BWI76_RS03425 - dihydrodipicolinate synthase family protein, at 704,635 to 705,546
_RS03425
BWI76_RS03430: BWI76_RS03430 - short-chain dehydrogenase, at 705,540 to 706,367
_RS03430
BWI76_RS03435: BWI76_RS03435 - mandelate racemase/muconate lactonizing protein, at 706,391 to 707,494
_RS03435
Position (kb) 705
706
707 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 704.626 kb on - strand at 704.626 kb on - strand at 704.673 kb on + strand at 704.862 kb on + strand, within BWI76_RS03425 at 704.912 kb on - strand, within BWI76_RS03425 at 705.065 kb on + strand, within BWI76_RS03425 at 705.184 kb on - strand, within BWI76_RS03425 at 705.249 kb on - strand, within BWI76_RS03425 at 705.262 kb on - strand, within BWI76_RS03425 at 705.420 kb on - strand, within BWI76_RS03425 at 705.662 kb on + strand, within BWI76_RS03430 at 705.698 kb on + strand, within BWI76_RS03430 at 706.000 kb on - strand, within BWI76_RS03430 at 706.062 kb on + strand, within BWI76_RS03430 at 706.065 kb on - strand, within BWI76_RS03430 at 706.128 kb on + strand, within BWI76_RS03430 at 706.187 kb on - strand, within BWI76_RS03430 at 706.280 kb on - strand, within BWI76_RS03430 at 706.290 kb on - strand at 706.305 kb on - strand at 706.667 kb on + strand, within BWI76_RS03435 at 706.714 kb on + strand, within BWI76_RS03435 at 706.740 kb on + strand, within BWI76_RS03435 at 707.059 kb on + strand, within BWI76_RS03435 at 707.165 kb on - strand, within BWI76_RS03435 at 707.277 kb on - strand, within BWI76_RS03435
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 704,626 - -0.2 704,626 - -0.4 704,673 + +0.8 704,862 + BWI76_RS03425 0.25 -3.4 704,912 - BWI76_RS03425 0.30 -0.1 705,065 + BWI76_RS03425 0.47 -0.2 705,184 - BWI76_RS03425 0.60 +0.5 705,249 - BWI76_RS03425 0.67 -0.5 705,262 - BWI76_RS03425 0.69 -0.8 705,420 - BWI76_RS03425 0.86 -0.7 705,662 + BWI76_RS03430 0.15 -0.7 705,698 + BWI76_RS03430 0.19 -1.0 706,000 - BWI76_RS03430 0.56 -1.5 706,062 + BWI76_RS03430 0.63 -1.0 706,065 - BWI76_RS03430 0.63 -1.2 706,128 + BWI76_RS03430 0.71 -1.4 706,187 - BWI76_RS03430 0.78 -1.1 706,280 - BWI76_RS03430 0.89 -0.8 706,290 - -1.0 706,305 - +0.6 706,667 + BWI76_RS03435 0.25 +0.4 706,714 + BWI76_RS03435 0.29 -1.0 706,740 + BWI76_RS03435 0.32 +0.0 707,059 + BWI76_RS03435 0.61 -0.5 707,165 - BWI76_RS03435 0.70 +0.3 707,277 - BWI76_RS03435 0.80 -1.0
Or see this region's nucleotide sequence