Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02875 and BWI76_RS02880 are separated by 12 nucleotides BWI76_RS02880 and BWI76_RS02885 are separated by 42 nucleotides BWI76_RS02885 and BWI76_RS02890 overlap by 17 nucleotides
BWI76_RS02875: BWI76_RS02875 - membrane protein, at 587,058 to 587,834
_RS02875
BWI76_RS02880: BWI76_RS02880 - membrane protein, at 587,847 to 588,491
_RS02880
BWI76_RS02885: BWI76_RS02885 - dihydroorotase, at 588,534 to 589,667
_RS02885
BWI76_RS02890: BWI76_RS02890 - L-seryl-tRNA selenium transferase, at 589,651 to 590,769
_RS02890
Position (kb) 588
589
590 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 587.615 kb on + strand, within BWI76_RS02875 at 587.640 kb on - strand, within BWI76_RS02875 at 587.645 kb on - strand, within BWI76_RS02875 at 587.670 kb on - strand, within BWI76_RS02875 at 587.810 kb on - strand at 587.863 kb on - strand at 587.886 kb on + strand at 587.914 kb on - strand, within BWI76_RS02880 at 587.942 kb on - strand, within BWI76_RS02880 at 588.116 kb on - strand, within BWI76_RS02880 at 588.118 kb on + strand, within BWI76_RS02880 at 588.270 kb on + strand, within BWI76_RS02880 at 588.369 kb on - strand, within BWI76_RS02880 at 588.554 kb on - strand at 588.571 kb on + strand at 588.624 kb on - strand at 588.684 kb on + strand, within BWI76_RS02885 at 588.773 kb on - strand, within BWI76_RS02885 at 588.863 kb on + strand, within BWI76_RS02885 at 588.871 kb on - strand, within BWI76_RS02885 at 588.871 kb on - strand, within BWI76_RS02885 at 588.913 kb on - strand, within BWI76_RS02885 at 589.224 kb on + strand, within BWI76_RS02885 at 589.433 kb on - strand, within BWI76_RS02885 at 589.525 kb on - strand, within BWI76_RS02885 at 589.542 kb on + strand, within BWI76_RS02885 at 589.932 kb on + strand, within BWI76_RS02890 at 589.971 kb on + strand, within BWI76_RS02890 at 589.971 kb on + strand, within BWI76_RS02890 at 590.493 kb on + strand, within BWI76_RS02890 at 590.527 kb on + strand, within BWI76_RS02890 at 590.527 kb on + strand, within BWI76_RS02890 at 590.527 kb on + strand, within BWI76_RS02890 at 590.527 kb on + strand, within BWI76_RS02890 at 590.527 kb on + strand, within BWI76_RS02890 at 590.527 kb on + strand, within BWI76_RS02890 at 590.532 kb on + strand, within BWI76_RS02890 at 590.535 kb on - strand, within BWI76_RS02890 at 590.535 kb on - strand, within BWI76_RS02890 at 590.605 kb on + strand, within BWI76_RS02890 at 590.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 587,615 + BWI76_RS02875 0.72 +3.7 587,640 - BWI76_RS02875 0.75 +0.7 587,645 - BWI76_RS02875 0.76 +1.1 587,670 - BWI76_RS02875 0.79 -0.7 587,810 - +0.7 587,863 - -0.7 587,886 + -1.2 587,914 - BWI76_RS02880 0.10 +0.4 587,942 - BWI76_RS02880 0.15 -0.2 588,116 - BWI76_RS02880 0.42 +2.0 588,118 + BWI76_RS02880 0.42 -0.5 588,270 + BWI76_RS02880 0.66 +0.9 588,369 - BWI76_RS02880 0.81 +0.6 588,554 - -1.6 588,571 + -0.5 588,624 - +0.2 588,684 + BWI76_RS02885 0.13 -1.7 588,773 - BWI76_RS02885 0.21 -2.6 588,863 + BWI76_RS02885 0.29 +0.9 588,871 - BWI76_RS02885 0.30 -0.7 588,871 - BWI76_RS02885 0.30 -0.4 588,913 - BWI76_RS02885 0.33 -0.1 589,224 + BWI76_RS02885 0.61 -0.4 589,433 - BWI76_RS02885 0.79 -1.3 589,525 - BWI76_RS02885 0.87 -0.9 589,542 + BWI76_RS02885 0.89 -0.1 589,932 + BWI76_RS02890 0.25 +0.4 589,971 + BWI76_RS02890 0.29 -0.1 589,971 + BWI76_RS02890 0.29 -1.7 590,493 + BWI76_RS02890 0.75 -0.4 590,527 + BWI76_RS02890 0.78 +0.1 590,527 + BWI76_RS02890 0.78 +1.4 590,527 + BWI76_RS02890 0.78 -0.1 590,527 + BWI76_RS02890 0.78 -0.1 590,527 + BWI76_RS02890 0.78 +0.5 590,527 + BWI76_RS02890 0.78 +0.0 590,532 + BWI76_RS02890 0.79 +0.8 590,535 - BWI76_RS02890 0.79 +1.0 590,535 - BWI76_RS02890 0.79 +0.2 590,605 + BWI76_RS02890 0.85 +0.8 590,662 - -0.8
Or see this region's nucleotide sequence