Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02260 and BWI76_RS02265 are separated by 149 nucleotides BWI76_RS02265 and BWI76_RS02270 are separated by 224 nucleotides
BWI76_RS02260: BWI76_RS02260 - DNA-binding protein, at 464,785 to 465,705
_RS02260
BWI76_RS02265: BWI76_RS02265 - putative transmembrane protein, at 465,855 to 466,535
_RS02265
BWI76_RS02270: BWI76_RS02270 - DSBA oxidoreductase, at 466,760 to 467,542
_RS02270
Position (kb) 465
466
467 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 464.934 kb on + strand, within BWI76_RS02260 at 464.934 kb on + strand, within BWI76_RS02260 at 465.097 kb on - strand, within BWI76_RS02260 at 465.279 kb on + strand, within BWI76_RS02260 at 465.435 kb on + strand, within BWI76_RS02260 at 465.437 kb on + strand, within BWI76_RS02260 at 465.445 kb on - strand, within BWI76_RS02260 at 465.536 kb on - strand, within BWI76_RS02260 at 465.608 kb on - strand, within BWI76_RS02260 at 465.647 kb on - strand at 465.647 kb on - strand at 465.647 kb on - strand at 465.801 kb on - strand at 465.853 kb on + strand at 465.907 kb on - strand at 465.907 kb on - strand at 465.925 kb on - strand, within BWI76_RS02265 at 466.201 kb on - strand, within BWI76_RS02265 at 466.315 kb on - strand, within BWI76_RS02265 at 466.421 kb on + strand, within BWI76_RS02265 at 466.429 kb on - strand, within BWI76_RS02265 at 466.434 kb on - strand, within BWI76_RS02265 at 466.589 kb on + strand at 466.589 kb on + strand at 466.589 kb on + strand at 466.591 kb on - strand at 466.597 kb on - strand at 466.606 kb on - strand at 466.614 kb on + strand at 466.695 kb on + strand at 466.740 kb on - strand at 466.859 kb on + strand, within BWI76_RS02270 at 466.926 kb on - strand, within BWI76_RS02270 at 466.958 kb on + strand, within BWI76_RS02270 at 466.971 kb on - strand, within BWI76_RS02270 at 466.993 kb on - strand, within BWI76_RS02270 at 467.193 kb on - strand, within BWI76_RS02270 at 467.256 kb on - strand, within BWI76_RS02270
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 464,934 + BWI76_RS02260 0.16 -0.8 464,934 + BWI76_RS02260 0.16 -0.5 465,097 - BWI76_RS02260 0.34 +0.2 465,279 + BWI76_RS02260 0.54 -0.3 465,435 + BWI76_RS02260 0.71 +1.0 465,437 + BWI76_RS02260 0.71 +0.6 465,445 - BWI76_RS02260 0.72 -1.4 465,536 - BWI76_RS02260 0.82 -1.2 465,608 - BWI76_RS02260 0.89 -0.3 465,647 - -1.4 465,647 - +0.9 465,647 - +1.2 465,801 - -0.2 465,853 + -0.6 465,907 - -1.1 465,907 - -1.1 465,925 - BWI76_RS02265 0.10 +0.3 466,201 - BWI76_RS02265 0.51 -0.3 466,315 - BWI76_RS02265 0.68 -1.2 466,421 + BWI76_RS02265 0.83 -1.5 466,429 - BWI76_RS02265 0.84 -1.0 466,434 - BWI76_RS02265 0.85 -0.1 466,589 + -0.4 466,589 + -2.3 466,589 + +1.5 466,591 - -0.5 466,597 - -1.6 466,606 - -1.0 466,614 + -0.1 466,695 + -3.4 466,740 - +2.3 466,859 + BWI76_RS02270 0.13 -1.2 466,926 - BWI76_RS02270 0.21 -0.1 466,958 + BWI76_RS02270 0.25 -0.7 466,971 - BWI76_RS02270 0.27 -2.6 466,993 - BWI76_RS02270 0.30 -1.2 467,193 - BWI76_RS02270 0.55 -0.6 467,256 - BWI76_RS02270 0.63 +3.4
Or see this region's nucleotide sequence