Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02180 and BWI76_RS02185 are separated by 33 nucleotides BWI76_RS02185 and BWI76_RS02190 are separated by 14 nucleotides BWI76_RS02190 and BWI76_RS02195 overlap by 1 nucleotides
BWI76_RS02180: BWI76_RS02180 - hypothetical protein, at 453,043 to 453,375
_RS02180
BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167
_RS02185
BWI76_RS02190: BWI76_RS02190 - ribose 1,5-bisphosphokinase, at 454,182 to 454,745
_RS02190
BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881
_RS02195
Position (kb) 454
455 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 453.224 kb on - strand, within BWI76_RS02180 at 453.304 kb on + strand, within BWI76_RS02180 at 453.504 kb on + strand at 453.537 kb on + strand, within BWI76_RS02185 at 453.705 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.830 kb on + strand, within BWI76_RS02185 at 453.848 kb on - strand, within BWI76_RS02185 at 453.943 kb on + strand, within BWI76_RS02185 at 454.169 kb on - strand at 454.274 kb on + strand, within BWI76_RS02190 at 454.343 kb on + strand, within BWI76_RS02190 at 454.559 kb on + strand, within BWI76_RS02190 at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.763 kb on - strand at 454.797 kb on + strand at 454.920 kb on - strand at 454.931 kb on + strand, within BWI76_RS02195 at 455.083 kb on - strand, within BWI76_RS02195 at 455.091 kb on - strand, within BWI76_RS02195 at 455.298 kb on - strand, within BWI76_RS02195 at 455.367 kb on + strand, within BWI76_RS02195 at 455.479 kb on + strand, within BWI76_RS02195 at 455.618 kb on - strand, within BWI76_RS02195 at 455.656 kb on - strand, within BWI76_RS02195 at 455.667 kb on + strand, within BWI76_RS02195 at 455.680 kb on - strand, within BWI76_RS02195
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 453,224 - BWI76_RS02180 0.54 +1.8 453,304 + BWI76_RS02180 0.78 +0.2 453,504 + +0.9 453,537 + BWI76_RS02185 0.17 +0.0 453,705 - BWI76_RS02185 0.39 +0.7 453,803 - BWI76_RS02185 0.52 +0.7 453,803 - BWI76_RS02185 0.52 -1.0 453,830 + BWI76_RS02185 0.55 -0.6 453,848 - BWI76_RS02185 0.58 -2.6 453,943 + BWI76_RS02185 0.70 -1.2 454,169 - +0.3 454,274 + BWI76_RS02190 0.16 -2.5 454,343 + BWI76_RS02190 0.29 -1.3 454,559 + BWI76_RS02190 0.67 -0.8 454,695 + +0.8 454,695 + -1.7 454,695 + -1.4 454,695 + -1.2 454,763 - +0.9 454,797 + -0.1 454,920 - -0.1 454,931 + BWI76_RS02195 0.16 +1.3 455,083 - BWI76_RS02195 0.30 +0.1 455,091 - BWI76_RS02195 0.30 -2.1 455,298 - BWI76_RS02195 0.49 -0.2 455,367 + BWI76_RS02195 0.55 -0.0 455,479 + BWI76_RS02195 0.65 -0.8 455,618 - BWI76_RS02195 0.77 +0.6 455,656 - BWI76_RS02195 0.80 +2.9 455,667 + BWI76_RS02195 0.81 -1.0 455,680 - BWI76_RS02195 0.82 +1.8
Or see this region's nucleotide sequence