Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01790 and BWI76_RS01795 are separated by 121 nucleotides BWI76_RS01795 and BWI76_RS01800 are separated by 282 nucleotides BWI76_RS01800 and BWI76_RS01805 are separated by 45 nucleotides BWI76_RS01805 and BWI76_RS01810 are separated by 8 nucleotides
BWI76_RS01790: BWI76_RS01790 - lysine-sensitive aspartokinase 3, at 365,131 to 366,480
_RS01790
BWI76_RS01795: BWI76_RS01795 - hypothetical protein, at 366,602 to 366,964
_RS01795
BWI76_RS01800: BWI76_RS01800 - glucose-6-phosphate isomerase, at 367,247 to 368,896
_RS01800
BWI76_RS01805: BWI76_RS01805 - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 368,942 to 369,397
_RS01805
BWI76_RS01810: BWI76_RS01810 - N-acetyltransferase, at 369,406 to 369,915
_RS01810
Position (kb) 367
368
369 Strain fitness (log2 ratio) -2
-1
0
1 at 366.253 kb on + strand, within BWI76_RS01790 at 366.418 kb on + strand at 366.663 kb on + strand, within BWI76_RS01795 at 366.681 kb on + strand, within BWI76_RS01795 at 366.801 kb on + strand, within BWI76_RS01795 at 366.931 kb on + strand at 366.938 kb on - strand at 366.990 kb on - strand at 367.238 kb on + strand at 367.265 kb on + strand at 367.443 kb on - strand, within BWI76_RS01800 at 367.532 kb on + strand, within BWI76_RS01800 at 367.566 kb on - strand, within BWI76_RS01800 at 367.772 kb on - strand, within BWI76_RS01800 at 368.147 kb on + strand, within BWI76_RS01800 at 368.527 kb on + strand, within BWI76_RS01800 at 368.604 kb on + strand, within BWI76_RS01800 at 368.758 kb on - strand at 368.765 kb on - strand at 368.862 kb on + strand at 368.899 kb on + strand at 369.126 kb on + strand, within BWI76_RS01805 at 369.134 kb on - strand, within BWI76_RS01805 at 369.314 kb on + strand, within BWI76_RS01805 at 369.322 kb on + strand, within BWI76_RS01805 at 369.388 kb on + strand at 369.388 kb on + strand at 369.388 kb on + strand at 369.503 kb on - strand, within BWI76_RS01810 at 369.569 kb on - strand, within BWI76_RS01810 at 369.599 kb on + strand, within BWI76_RS01810 at 369.637 kb on - strand, within BWI76_RS01810 at 369.689 kb on - strand, within BWI76_RS01810 at 369.809 kb on - strand, within BWI76_RS01810 at 369.841 kb on - strand, within BWI76_RS01810 at 369.884 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 366,253 + BWI76_RS01790 0.83 -1.5 366,418 + -0.9 366,663 + BWI76_RS01795 0.17 -0.5 366,681 + BWI76_RS01795 0.22 +0.2 366,801 + BWI76_RS01795 0.55 -1.1 366,931 + -0.7 366,938 - +0.3 366,990 - -1.9 367,238 + -2.5 367,265 + -1.6 367,443 - BWI76_RS01800 0.12 -2.5 367,532 + BWI76_RS01800 0.17 -0.4 367,566 - BWI76_RS01800 0.19 -0.8 367,772 - BWI76_RS01800 0.32 -2.2 368,147 + BWI76_RS01800 0.55 -1.1 368,527 + BWI76_RS01800 0.78 -1.8 368,604 + BWI76_RS01800 0.82 -1.4 368,758 - -0.4 368,765 - -2.6 368,862 + -2.2 368,899 + +0.7 369,126 + BWI76_RS01805 0.40 -1.7 369,134 - BWI76_RS01805 0.42 -0.3 369,314 + BWI76_RS01805 0.82 +1.3 369,322 + BWI76_RS01805 0.83 -0.6 369,388 + -0.0 369,388 + -0.3 369,388 + -0.7 369,503 - BWI76_RS01810 0.19 -0.5 369,569 - BWI76_RS01810 0.32 +1.0 369,599 + BWI76_RS01810 0.38 -0.0 369,637 - BWI76_RS01810 0.45 +0.9 369,689 - BWI76_RS01810 0.55 +1.6 369,809 - BWI76_RS01810 0.79 +1.5 369,841 - BWI76_RS01810 0.85 +0.4 369,884 - -0.6
Or see this region's nucleotide sequence