Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04620

Experiment: Dictyostellum sp.mixed culture-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04615 and BWI76_RS04620 are separated by 162 nucleotidesBWI76_RS04620 and BWI76_RS04625 are separated by 87 nucleotidesBWI76_RS04625 and BWI76_RS04630 are separated by 231 nucleotides BWI76_RS04615: BWI76_RS04615 - thiamine transporter substrate binding subunit, at 960,777 to 961,760 _RS04615 BWI76_RS04620: BWI76_RS04620 - transcriptional regulator SgrR, at 961,923 to 963,578 _RS04620 BWI76_RS04625: BWI76_RS04625 - glucose uptake inhibitor SgrT, at 963,666 to 963,818 _RS04625 BWI76_RS04630: BWI76_RS04630 - sugar efflux transporter SetB, at 964,050 to 965,234 _RS04630 Position (kb) 961 962 963 964Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 961.024 kb on + strand, within BWI76_RS04615at 961.132 kb on - strand, within BWI76_RS04615at 961.132 kb on - strand, within BWI76_RS04615at 961.321 kb on + strand, within BWI76_RS04615at 961.329 kb on - strand, within BWI76_RS04615at 961.495 kb on + strand, within BWI76_RS04615at 961.674 kb on - strandat 962.083 kb on + strandat 962.166 kb on - strand, within BWI76_RS04620at 962.225 kb on - strand, within BWI76_RS04620at 962.234 kb on + strand, within BWI76_RS04620at 962.615 kb on + strand, within BWI76_RS04620at 962.759 kb on + strand, within BWI76_RS04620at 962.761 kb on + strand, within BWI76_RS04620at 962.837 kb on - strand, within BWI76_RS04620at 962.885 kb on + strand, within BWI76_RS04620at 962.885 kb on + strand, within BWI76_RS04620at 963.194 kb on + strand, within BWI76_RS04620at 963.230 kb on + strand, within BWI76_RS04620at 963.364 kb on + strand, within BWI76_RS04620at 963.364 kb on + strand, within BWI76_RS04620at 963.397 kb on + strand, within BWI76_RS04620at 963.774 kb on + strand, within BWI76_RS04625at 963.785 kb on + strand, within BWI76_RS04625at 963.805 kb on + strandat 963.805 kb on + strandat 963.813 kb on - strandat 963.827 kb on + strandat 963.827 kb on + strandat 963.827 kb on + strandat 963.975 kb on + strandat 963.975 kb on + strandat 963.975 kb on + strandat 964.159 kb on + strandat 964.203 kb on + strand, within BWI76_RS04630at 964.208 kb on + strand, within BWI76_RS04630at 964.246 kb on + strand, within BWI76_RS04630at 964.263 kb on + strand, within BWI76_RS04630at 964.365 kb on - strand, within BWI76_RS04630at 964.365 kb on - strand, within BWI76_RS04630at 964.386 kb on - strand, within BWI76_RS04630at 964.386 kb on - strand, within BWI76_RS04630at 964.464 kb on + strand, within BWI76_RS04630at 964.496 kb on + strand, within BWI76_RS04630at 964.524 kb on + strand, within BWI76_RS04630

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Per-strain Table

Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2
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961,024 + BWI76_RS04615 0.25 +1.4
961,132 - BWI76_RS04615 0.36 +1.2
961,132 - BWI76_RS04615 0.36 +0.6
961,321 + BWI76_RS04615 0.55 +0.9
961,329 - BWI76_RS04615 0.56 +0.6
961,495 + BWI76_RS04615 0.73 +0.0
961,674 - -0.5
962,083 + +1.2
962,166 - BWI76_RS04620 0.15 -0.8
962,225 - BWI76_RS04620 0.18 +0.6
962,234 + BWI76_RS04620 0.19 -1.5
962,615 + BWI76_RS04620 0.42 -0.9
962,759 + BWI76_RS04620 0.50 +0.8
962,761 + BWI76_RS04620 0.51 -0.5
962,837 - BWI76_RS04620 0.55 -1.2
962,885 + BWI76_RS04620 0.58 -0.7
962,885 + BWI76_RS04620 0.58 -0.6
963,194 + BWI76_RS04620 0.77 -0.9
963,230 + BWI76_RS04620 0.79 +3.2
963,364 + BWI76_RS04620 0.87 -0.1
963,364 + BWI76_RS04620 0.87 +0.2
963,397 + BWI76_RS04620 0.89 -3.1
963,774 + BWI76_RS04625 0.71 +1.7
963,785 + BWI76_RS04625 0.78 -0.2
963,805 + -2.2
963,805 + +0.0
963,813 - +0.6
963,827 + +0.3
963,827 + +1.6
963,827 + +0.3
963,975 + -0.2
963,975 + +3.1
963,975 + -0.6
964,159 + +3.6
964,203 + BWI76_RS04630 0.13 +0.7
964,208 + BWI76_RS04630 0.13 +1.2
964,246 + BWI76_RS04630 0.17 +1.1
964,263 + BWI76_RS04630 0.18 +0.2
964,365 - BWI76_RS04630 0.27 -1.0
964,365 - BWI76_RS04630 0.27 -0.0
964,386 - BWI76_RS04630 0.28 -0.3
964,386 - BWI76_RS04630 0.28 +0.3
964,464 + BWI76_RS04630 0.35 -0.5
964,496 + BWI76_RS04630 0.38 +0.6
964,524 + BWI76_RS04630 0.40 +0.5

Or see this region's nucleotide sequence