Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25240 and BWI76_RS25245 are separated by 14 nucleotides BWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotides BWI76_RS25250 and BWI76_RS25255 overlap by 32 nucleotides BWI76_RS25255 and BWI76_RS25260 are separated by 6 nucleotides
BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400
_RS25240
BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981
_RS25245
BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553
_RS25250
BWI76_RS25255: BWI76_RS25255 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 5,250,522 to 5,251,067
_RS25255
BWI76_RS25260: BWI76_RS25260 - ABC transporter ATP-binding protein, at 5,251,074 to 5,251,799
_RS25260
Position (kb) 5249
5250
5251 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5249.215 kb on - strand, within BWI76_RS25240 at 5249.395 kb on + strand at 5249.446 kb on + strand at 5249.595 kb on + strand, within BWI76_RS25245 at 5249.720 kb on + strand, within BWI76_RS25245 at 5249.823 kb on + strand, within BWI76_RS25245 at 5249.945 kb on + strand at 5250.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 5,249,215 - BWI76_RS25240 0.81 -0.3 5,249,395 + -0.6 5,249,446 + +0.1 5,249,595 + BWI76_RS25245 0.32 +1.0 5,249,720 + BWI76_RS25245 0.54 -2.0 5,249,823 + BWI76_RS25245 0.72 +2.1 5,249,945 + -0.9 5,250,508 + -1.2
Or see this region's nucleotide sequence