Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27215

Experiment: Tetrahymena sp., Mixed culture-1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27210 and BWI76_RS27215 overlap by 1 nucleotidesBWI76_RS27215 and BWI76_RS27220 are separated by 59 nucleotidesBWI76_RS27220 and BWI76_RS27225 are separated by 111 nucleotides BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195 _RS27210 BWI76_RS27215: BWI76_RS27215 - protein-export protein SecB, at 5,649,195 to 5,649,662 _RS27215 BWI76_RS27220: BWI76_RS27220 - glutaredoxin 3, at 5,649,722 to 5,649,973 _RS27220 BWI76_RS27225: BWI76_RS27225 - rhodanese-like domain-containing protein, at 5,650,085 to 5,650,516 _RS27225 Position (kb) 5649 5650Strain fitness (log2 ratio) -2 -1 0 1 2at 5649.738 kb on - strandat 5649.898 kb on - strand, within BWI76_RS27220at 5649.958 kb on - strandat 5650.117 kb on - strandat 5650.164 kb on - strand, within BWI76_RS27225at 5650.175 kb on - strand, within BWI76_RS27225at 5650.472 kb on + strand, within BWI76_RS27225at 5650.561 kb on + strandat 5650.569 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1
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5,649,738 - +0.2
5,649,898 - BWI76_RS27220 0.70 +2.6
5,649,958 - +0.9
5,650,117 - +0.2
5,650,164 - BWI76_RS27225 0.18 -0.5
5,650,175 - BWI76_RS27225 0.21 -1.1
5,650,472 + BWI76_RS27225 0.90 -2.5
5,650,561 + -1.0
5,650,569 - -2.3

Or see this region's nucleotide sequence