Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27130 and BWI76_RS27135 are separated by 107 nucleotides BWI76_RS27135 and BWI76_RS27140 overlap by 4 nucleotides
BWI76_RS27130: BWI76_RS27130 - hypothetical protein, at 5,629,874 to 5,631,835
_RS27130
BWI76_RS27135: BWI76_RS27135 - selenocysteine-specific translation factor, at 5,631,943 to 5,633,784
_RS27135
BWI76_RS27140: BWI76_RS27140 - L-selenocysteinyl-tRNA(Sec) synthase, at 5,633,781 to 5,635,169
_RS27140
Position (kb)
5631
5632
5633
5634 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5630.966 kb on + strand, within BWI76_RS27130 at 5630.968 kb on + strand, within BWI76_RS27130 at 5630.968 kb on + strand, within BWI76_RS27130 at 5630.968 kb on + strand, within BWI76_RS27130 at 5630.968 kb on + strand, within BWI76_RS27130 at 5630.976 kb on - strand, within BWI76_RS27130 at 5630.976 kb on - strand, within BWI76_RS27130 at 5631.084 kb on - strand, within BWI76_RS27130 at 5631.137 kb on + strand, within BWI76_RS27130 at 5631.170 kb on + strand, within BWI76_RS27130 at 5631.177 kb on + strand, within BWI76_RS27130 at 5631.177 kb on + strand, within BWI76_RS27130 at 5631.185 kb on - strand, within BWI76_RS27130 at 5631.291 kb on - strand, within BWI76_RS27130 at 5631.311 kb on + strand, within BWI76_RS27130 at 5631.312 kb on - strand, within BWI76_RS27130 at 5631.461 kb on - strand, within BWI76_RS27130 at 5631.515 kb on + strand, within BWI76_RS27130 at 5631.596 kb on - strand, within BWI76_RS27130 at 5631.805 kb on - strand at 5631.838 kb on - strand at 5631.844 kb on - strand at 5631.863 kb on - strand at 5631.880 kb on + strand at 5631.902 kb on + strand at 5631.976 kb on - strand at 5631.977 kb on - strand at 5631.977 kb on - strand at 5631.977 kb on - strand at 5632.032 kb on - strand at 5632.101 kb on - strand at 5632.182 kb on - strand, within BWI76_RS27135 at 5632.198 kb on + strand, within BWI76_RS27135 at 5632.206 kb on - strand, within BWI76_RS27135 at 5632.206 kb on - strand, within BWI76_RS27135 at 5632.259 kb on + strand, within BWI76_RS27135 at 5632.363 kb on + strand at 5632.363 kb on + strand at 5632.610 kb on + strand, within BWI76_RS27135 at 5632.634 kb on + strand, within BWI76_RS27135 at 5632.686 kb on + strand, within BWI76_RS27135 at 5632.888 kb on - strand, within BWI76_RS27135 at 5632.994 kb on - strand, within BWI76_RS27135 at 5633.113 kb on - strand, within BWI76_RS27135 at 5633.251 kb on + strand, within BWI76_RS27135 at 5633.276 kb on + strand, within BWI76_RS27135 at 5633.278 kb on - strand, within BWI76_RS27135 at 5633.325 kb on + strand, within BWI76_RS27135 at 5633.326 kb on + strand, within BWI76_RS27135 at 5633.356 kb on - strand, within BWI76_RS27135 at 5633.380 kb on - strand, within BWI76_RS27135 at 5633.401 kb on - strand, within BWI76_RS27135 at 5633.414 kb on + strand, within BWI76_RS27135 at 5633.657 kb on + strand at 5633.824 kb on - strand at 5633.829 kb on - strand at 5633.831 kb on + strand at 5633.834 kb on - strand at 5633.834 kb on - strand at 5633.836 kb on + strand at 5633.890 kb on - strand at 5633.936 kb on + strand, within BWI76_RS27140 at 5633.943 kb on + strand, within BWI76_RS27140 at 5633.946 kb on - strand, within BWI76_RS27140 at 5634.120 kb on - strand, within BWI76_RS27140 at 5634.163 kb on + strand, within BWI76_RS27140 at 5634.274 kb on - strand, within BWI76_RS27140 at 5634.360 kb on + strand, within BWI76_RS27140 at 5634.490 kb on - strand, within BWI76_RS27140 at 5634.586 kb on + strand, within BWI76_RS27140
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 5,630,966 + BWI76_RS27130 0.56 +0.1 5,630,968 + BWI76_RS27130 0.56 +0.9 5,630,968 + BWI76_RS27130 0.56 +0.4 5,630,968 + BWI76_RS27130 0.56 -0.8 5,630,968 + BWI76_RS27130 0.56 -0.8 5,630,976 - BWI76_RS27130 0.56 -2.0 5,630,976 - BWI76_RS27130 0.56 -0.1 5,631,084 - BWI76_RS27130 0.62 -1.9 5,631,137 + BWI76_RS27130 0.64 +0.4 5,631,170 + BWI76_RS27130 0.66 +2.6 5,631,177 + BWI76_RS27130 0.66 +1.6 5,631,177 + BWI76_RS27130 0.66 -0.2 5,631,185 - BWI76_RS27130 0.67 -2.4 5,631,291 - BWI76_RS27130 0.72 +0.6 5,631,311 + BWI76_RS27130 0.73 +0.4 5,631,312 - BWI76_RS27130 0.73 -1.1 5,631,461 - BWI76_RS27130 0.81 -0.5 5,631,515 + BWI76_RS27130 0.84 +0.3 5,631,596 - BWI76_RS27130 0.88 +1.4 5,631,805 - +0.1 5,631,838 - -0.9 5,631,844 - -1.7 5,631,863 - -0.3 5,631,880 + -0.2 5,631,902 + +0.7 5,631,976 - +0.2 5,631,977 - +2.6 5,631,977 - +0.5 5,631,977 - -0.4 5,632,032 - -0.2 5,632,101 - +1.6 5,632,182 - BWI76_RS27135 0.13 -0.1 5,632,198 + BWI76_RS27135 0.14 +3.1 5,632,206 - BWI76_RS27135 0.14 +1.7 5,632,206 - BWI76_RS27135 0.14 -2.9 5,632,259 + BWI76_RS27135 0.17 +0.6 5,632,363 + +2.2 5,632,363 + +0.6 5,632,610 + BWI76_RS27135 0.36 -0.0 5,632,634 + BWI76_RS27135 0.38 -2.5 5,632,686 + BWI76_RS27135 0.40 +1.5 5,632,888 - BWI76_RS27135 0.51 -0.7 5,632,994 - BWI76_RS27135 0.57 -0.2 5,633,113 - BWI76_RS27135 0.64 +1.8 5,633,251 + BWI76_RS27135 0.71 +0.0 5,633,276 + BWI76_RS27135 0.72 -1.8 5,633,278 - BWI76_RS27135 0.72 +0.1 5,633,325 + BWI76_RS27135 0.75 -1.0 5,633,326 + BWI76_RS27135 0.75 +1.1 5,633,356 - BWI76_RS27135 0.77 -0.6 5,633,380 - BWI76_RS27135 0.78 -0.3 5,633,401 - BWI76_RS27135 0.79 -1.2 5,633,414 + BWI76_RS27135 0.80 +2.5 5,633,657 + +2.7 5,633,824 - -0.1 5,633,829 - +1.3 5,633,831 + -0.6 5,633,834 - +1.8 5,633,834 - +1.4 5,633,836 + +1.1 5,633,890 - +1.2 5,633,936 + BWI76_RS27140 0.11 -0.8 5,633,943 + BWI76_RS27140 0.12 +0.4 5,633,946 - BWI76_RS27140 0.12 +0.2 5,634,120 - BWI76_RS27140 0.24 -0.2 5,634,163 + BWI76_RS27140 0.28 -0.6 5,634,274 - BWI76_RS27140 0.35 +0.5 5,634,360 + BWI76_RS27140 0.42 -0.8 5,634,490 - BWI76_RS27140 0.51 -1.0 5,634,586 + BWI76_RS27140 0.58 +0.4
Or see this region's nucleotide sequence