Experiment: Tetrahymena sp., Mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24520 and BWI76_RS24525 are separated by 8 nucleotides BWI76_RS24525 and BWI76_RS24530 are separated by 151 nucleotides BWI76_RS24530 and BWI76_RS24535 are separated by 46 nucleotides
BWI76_RS24520: BWI76_RS24520 - urease accessory protein UreF, at 5,111,823 to 5,112,497
_RS24520
BWI76_RS24525: BWI76_RS24525 - urease accessory protein UreG, at 5,112,506 to 5,113,123
_RS24525
BWI76_RS24530: BWI76_RS24530 - cytosine/purines uracil thiamine allantoin permease, at 5,113,275 to 5,114,882
_RS24530
BWI76_RS24535: BWI76_RS24535 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 5,114,929 to 5,115,942
_RS24535
Position (kb) 5113
5114
5115 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5112.392 kb on - strand, within BWI76_RS24520 at 5112.511 kb on - strand at 5112.582 kb on - strand, within BWI76_RS24525 at 5112.586 kb on + strand, within BWI76_RS24525 at 5112.594 kb on - strand, within BWI76_RS24525 at 5112.687 kb on - strand, within BWI76_RS24525 at 5112.857 kb on - strand, within BWI76_RS24525 at 5113.124 kb on + strand at 5113.124 kb on + strand at 5113.140 kb on + strand at 5113.140 kb on + strand at 5113.234 kb on - strand at 5113.241 kb on + strand at 5113.313 kb on + strand at 5113.329 kb on - strand at 5113.348 kb on + strand at 5113.356 kb on - strand at 5113.392 kb on - strand at 5113.539 kb on - strand, within BWI76_RS24530 at 5113.570 kb on - strand, within BWI76_RS24530 at 5113.570 kb on - strand, within BWI76_RS24530 at 5113.581 kb on - strand, within BWI76_RS24530 at 5113.721 kb on + strand, within BWI76_RS24530 at 5113.799 kb on + strand, within BWI76_RS24530 at 5113.941 kb on - strand, within BWI76_RS24530 at 5113.945 kb on + strand, within BWI76_RS24530 at 5113.953 kb on - strand, within BWI76_RS24530 at 5114.064 kb on + strand, within BWI76_RS24530 at 5114.064 kb on + strand, within BWI76_RS24530 at 5114.148 kb on + strand, within BWI76_RS24530 at 5114.150 kb on + strand, within BWI76_RS24530 at 5114.150 kb on + strand, within BWI76_RS24530 at 5114.156 kb on - strand at 5114.174 kb on - strand, within BWI76_RS24530 at 5114.229 kb on - strand, within BWI76_RS24530 at 5114.372 kb on - strand, within BWI76_RS24530 at 5114.381 kb on + strand, within BWI76_RS24530 at 5114.389 kb on - strand, within BWI76_RS24530 at 5114.428 kb on + strand, within BWI76_RS24530 at 5114.565 kb on + strand, within BWI76_RS24530 at 5114.710 kb on + strand, within BWI76_RS24530 at 5114.718 kb on - strand, within BWI76_RS24530 at 5114.757 kb on - strand at 5114.762 kb on - strand at 5114.864 kb on + strand at 5114.864 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetrahymena sp., Mixed culture-1 remove 5,112,392 - BWI76_RS24520 0.84 +1.2 5,112,511 - -0.5 5,112,582 - BWI76_RS24525 0.12 +0.8 5,112,586 + BWI76_RS24525 0.13 -0.5 5,112,594 - BWI76_RS24525 0.14 -1.1 5,112,687 - BWI76_RS24525 0.29 +0.5 5,112,857 - BWI76_RS24525 0.57 -0.6 5,113,124 + +0.8 5,113,124 + +1.3 5,113,140 + +0.3 5,113,140 + +0.7 5,113,234 - +0.9 5,113,241 + +0.7 5,113,313 + +1.5 5,113,329 - +0.0 5,113,348 + -1.4 5,113,356 - +1.3 5,113,392 - -0.2 5,113,539 - BWI76_RS24530 0.16 -0.4 5,113,570 - BWI76_RS24530 0.18 -1.2 5,113,570 - BWI76_RS24530 0.18 -0.9 5,113,581 - BWI76_RS24530 0.19 +0.7 5,113,721 + BWI76_RS24530 0.28 +0.0 5,113,799 + BWI76_RS24530 0.33 +0.1 5,113,941 - BWI76_RS24530 0.41 -0.8 5,113,945 + BWI76_RS24530 0.42 +1.2 5,113,953 - BWI76_RS24530 0.42 +0.5 5,114,064 + BWI76_RS24530 0.49 -0.8 5,114,064 + BWI76_RS24530 0.49 +0.1 5,114,148 + BWI76_RS24530 0.54 -0.5 5,114,150 + BWI76_RS24530 0.54 +1.7 5,114,150 + BWI76_RS24530 0.54 -1.2 5,114,156 - +2.2 5,114,174 - BWI76_RS24530 0.56 +2.2 5,114,229 - BWI76_RS24530 0.59 -0.1 5,114,372 - BWI76_RS24530 0.68 +1.0 5,114,381 + BWI76_RS24530 0.69 -1.9 5,114,389 - BWI76_RS24530 0.69 +0.0 5,114,428 + BWI76_RS24530 0.72 +2.4 5,114,565 + BWI76_RS24530 0.80 -0.2 5,114,710 + BWI76_RS24530 0.89 +0.4 5,114,718 - BWI76_RS24530 0.90 +0.2 5,114,757 - -2.1 5,114,762 - -0.1 5,114,864 + +1.9 5,114,864 + +1.5
Or see this region's nucleotide sequence