Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_692 and Pf6N2E2_693 are separated by 274 nucleotides Pf6N2E2_693 and Pf6N2E2_694 overlap by 6 nucleotides Pf6N2E2_694 and Pf6N2E2_695 are separated by 99 nucleotides
Pf6N2E2_692 - Glucarate dehydratase (EC 4.2.1.40), at 798,439 to 799,713
_692
Pf6N2E2_693 - hypothetical protein, at 799,988 to 800,125
_693
Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415
_694
Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011
_695
Position (kb) 800
801
802 Strain fitness (log2 ratio) -1
0
1 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.168 kb on + strand, within Pf6N2E2_692 at 799.927 kb on - strand at 799.952 kb on + strand at 799.952 kb on + strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.953 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 799.990 kb on - strand at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.055 kb on - strand, within Pf6N2E2_693 at 800.132 kb on - strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.169 kb on + strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.170 kb on - strand at 800.222 kb on + strand at 800.258 kb on + strand, within Pf6N2E2_694 at 800.273 kb on + strand, within Pf6N2E2_694 at 800.274 kb on - strand, within Pf6N2E2_694 at 800.309 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.354 kb on + strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.355 kb on - strand, within Pf6N2E2_694 at 800.480 kb on + strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.481 kb on - strand, within Pf6N2E2_694 at 800.814 kb on - strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.864 kb on + strand, within Pf6N2E2_694 at 800.925 kb on - strand, within Pf6N2E2_694 at 801.051 kb on - strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.485 kb on + strand at 801.561 kb on + strand at 801.561 kb on + strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.807 kb on + strand, within Pf6N2E2_695 at 801.957 kb on + strand, within Pf6N2E2_695 at 801.958 kb on - strand, within Pf6N2E2_695 at 802.124 kb on - strand, within Pf6N2E2_695 at 802.224 kb on + strand, within Pf6N2E2_695
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 799,168 + Pf6N2E2_692 0.57 -0.7 799,168 + Pf6N2E2_692 0.57 +0.3 799,927 - +1.5 799,952 + -0.7 799,952 + +1.3 799,953 - -0.7 799,953 - +0.3 799,953 - +1.2 799,990 - -0.7 799,990 - +0.5 799,990 - -0.7 800,055 - Pf6N2E2_693 0.49 +1.4 800,055 - Pf6N2E2_693 0.49 +0.3 800,055 - Pf6N2E2_693 0.49 +0.3 800,132 - +0.3 800,169 + +0.3 800,169 + -1.5 800,169 + +0.4 800,169 + +0.3 800,169 + -0.7 800,170 - +0.3 800,170 - +0.3 800,170 - +1.6 800,170 - +0.3 800,222 + +0.3 800,258 + Pf6N2E2_694 0.11 -0.7 800,273 + Pf6N2E2_694 0.12 +0.3 800,274 - Pf6N2E2_694 0.12 +0.3 800,309 + Pf6N2E2_694 0.15 +0.9 800,354 + Pf6N2E2_694 0.18 -0.7 800,354 + Pf6N2E2_694 0.18 +0.3 800,355 - Pf6N2E2_694 0.18 +0.9 800,355 - Pf6N2E2_694 0.18 +0.3 800,355 - Pf6N2E2_694 0.18 +0.3 800,355 - Pf6N2E2_694 0.18 -0.7 800,480 + Pf6N2E2_694 0.28 +0.3 800,481 - Pf6N2E2_694 0.28 +0.3 800,481 - Pf6N2E2_694 0.28 +0.3 800,481 - Pf6N2E2_694 0.28 +0.3 800,481 - Pf6N2E2_694 0.28 +0.3 800,814 - Pf6N2E2_694 0.54 -0.7 800,843 + Pf6N2E2_694 0.56 +0.3 800,843 + Pf6N2E2_694 0.56 +0.3 800,844 - Pf6N2E2_694 0.56 +0.3 800,844 - Pf6N2E2_694 0.56 +0.3 800,844 - Pf6N2E2_694 0.56 +0.3 800,864 + Pf6N2E2_694 0.57 -1.1 800,925 - Pf6N2E2_694 0.62 +0.3 801,051 - Pf6N2E2_694 0.72 +1.3 801,068 + Pf6N2E2_694 0.73 -1.7 801,068 + Pf6N2E2_694 0.73 -0.7 801,068 + Pf6N2E2_694 0.73 +1.1 801,485 + -0.7 801,561 + +0.3 801,561 + -0.7 801,562 - -0.7 801,562 - -0.7 801,562 - -1.3 801,807 + Pf6N2E2_695 0.20 +0.3 801,957 + Pf6N2E2_695 0.30 +0.3 801,958 - Pf6N2E2_695 0.30 +0.3 802,124 - Pf6N2E2_695 0.41 -1.7 802,224 + Pf6N2E2_695 0.47 -0.7
Or see this region's nucleotide sequence