Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_667

Experiment: Ying_Nucleobase14 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_666 and Pf6N2E2_667 overlap by 1 nucleotidesPf6N2E2_667 and Pf6N2E2_668 are separated by 55 nucleotides Pf6N2E2_666 - Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-), at 771,042 to 772,058 _666 Pf6N2E2_667 - Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-), at 772,058 to 773,170 _667 Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320 _668 Position (kb) 772 773 774Strain fitness (log2 ratio) -2 -1 0 1at 771.378 kb on + strand, within Pf6N2E2_666at 771.379 kb on - strand, within Pf6N2E2_666at 771.379 kb on - strand, within Pf6N2E2_666at 771.506 kb on - strand, within Pf6N2E2_666at 771.506 kb on - strand, within Pf6N2E2_666at 771.526 kb on - strand, within Pf6N2E2_666at 771.601 kb on - strand, within Pf6N2E2_666at 771.633 kb on + strand, within Pf6N2E2_666at 771.633 kb on + strand, within Pf6N2E2_666at 771.776 kb on - strand, within Pf6N2E2_666at 771.884 kb on + strand, within Pf6N2E2_666at 771.993 kb on + strandat 771.993 kb on + strandat 771.993 kb on + strandat 771.993 kb on + strandat 771.993 kb on + strandat 771.994 kb on - strandat 771.994 kb on - strandat 771.994 kb on - strandat 771.994 kb on - strandat 771.994 kb on - strandat 772.072 kb on + strandat 772.782 kb on - strand, within Pf6N2E2_667at 772.895 kb on + strand, within Pf6N2E2_667at 772.895 kb on + strand, within Pf6N2E2_667at 772.896 kb on - strand, within Pf6N2E2_667at 772.896 kb on - strand, within Pf6N2E2_667at 772.948 kb on - strand, within Pf6N2E2_667at 772.989 kb on + strand, within Pf6N2E2_667at 772.990 kb on - strand, within Pf6N2E2_667at 773.388 kb on + strand, within Pf6N2E2_668at 773.388 kb on + strand, within Pf6N2E2_668at 773.389 kb on - strand, within Pf6N2E2_668at 773.568 kb on + strand, within Pf6N2E2_668at 773.568 kb on + strand, within Pf6N2E2_668at 773.569 kb on - strand, within Pf6N2E2_668at 773.569 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.572 kb on - strand, within Pf6N2E2_668at 773.590 kb on - strand, within Pf6N2E2_668at 773.676 kb on + strand, within Pf6N2E2_668at 773.676 kb on + strand, within Pf6N2E2_668at 774.147 kb on + strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3
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771,378 + Pf6N2E2_666 0.33 +0.3
771,379 - Pf6N2E2_666 0.33 -1.3
771,379 - Pf6N2E2_666 0.33 +0.3
771,506 - Pf6N2E2_666 0.46 +0.3
771,506 - Pf6N2E2_666 0.46 -1.1
771,526 - Pf6N2E2_666 0.48 +0.3
771,601 - Pf6N2E2_666 0.55 -0.7
771,633 + Pf6N2E2_666 0.58 -0.4
771,633 + Pf6N2E2_666 0.58 +0.1
771,776 - Pf6N2E2_666 0.72 -0.3
771,884 + Pf6N2E2_666 0.83 +0.3
771,993 + -0.7
771,993 + -1.3
771,993 + +0.3
771,993 + +0.3
771,993 + +0.3
771,994 - -0.5
771,994 - +0.3
771,994 - +0.3
771,994 - -0.2
771,994 - -0.6
772,072 + +0.3
772,782 - Pf6N2E2_667 0.65 -1.0
772,895 + Pf6N2E2_667 0.75 -0.3
772,895 + Pf6N2E2_667 0.75 +1.7
772,896 - Pf6N2E2_667 0.75 +0.3
772,896 - Pf6N2E2_667 0.75 +0.3
772,948 - Pf6N2E2_667 0.80 -1.3
772,989 + Pf6N2E2_667 0.84 -0.2
772,990 - Pf6N2E2_667 0.84 -0.7
773,388 + Pf6N2E2_668 0.15 +0.3
773,388 + Pf6N2E2_668 0.15 -0.7
773,389 - Pf6N2E2_668 0.15 -0.7
773,568 + Pf6N2E2_668 0.31 -0.7
773,568 + Pf6N2E2_668 0.31 -1.4
773,569 - Pf6N2E2_668 0.31 -0.5
773,569 - Pf6N2E2_668 0.31 +0.3
773,572 - Pf6N2E2_668 0.32 +0.3
773,572 - Pf6N2E2_668 0.32 +0.3
773,572 - Pf6N2E2_668 0.32 -2.5
773,572 - Pf6N2E2_668 0.32 -0.7
773,590 - Pf6N2E2_668 0.33 +0.3
773,676 + Pf6N2E2_668 0.41 -0.9
773,676 + Pf6N2E2_668 0.41 +0.3
774,147 + Pf6N2E2_668 0.84 -0.7
774,148 - Pf6N2E2_668 0.84 +0.3
774,148 - Pf6N2E2_668 0.84 +0.3
774,148 - Pf6N2E2_668 0.84 +0.3

Or see this region's nucleotide sequence