Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_464

Experiment: Ying_Nucleobase14 rep C; time point 3

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf6N2E2_462 and Pf6N2E2_463 are separated by 167 nucleotidesPf6N2E2_463 and Pf6N2E2_464 are separated by 4 nucleotidesPf6N2E2_464 and Pf6N2E2_465 are separated by 68 nucleotidesPf6N2E2_465 and Pf6N2E2_466 are separated by 41 nucleotides Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486 _462 Pf6N2E2_463 - Transcriptional regulatory protein RstA, at 528,654 to 529,355 _463 Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661 _464 Pf6N2E2_465 - Probable transmembrane protein, at 530,730 to 531,371 _465 Pf6N2E2_466 - Nicotinamidase (EC 3.5.1.19), at 531,413 to 532,066 _466 Position (kb) 529 530 531Strain fitness (log2 ratio) -1 0 1 2at 528.391 kb on - strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.548 kb on + strandat 528.711 kb on + strandat 528.712 kb on - strandat 528.948 kb on + strand, within Pf6N2E2_463at 528.948 kb on + strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.995 kb on + strand, within Pf6N2E2_463at 529.131 kb on + strand, within Pf6N2E2_463at 529.132 kb on - strand, within Pf6N2E2_463at 529.132 kb on - strand, within Pf6N2E2_463at 529.171 kb on + strand, within Pf6N2E2_463at 529.171 kb on + strand, within Pf6N2E2_463at 529.172 kb on - strand, within Pf6N2E2_463at 529.172 kb on - strand, within Pf6N2E2_463at 529.172 kb on - strand, within Pf6N2E2_463at 529.406 kb on - strandat 529.420 kb on + strandat 529.421 kb on - strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.472 kb on - strandat 529.484 kb on - strandat 529.774 kb on + strand, within Pf6N2E2_464at 529.775 kb on - strand, within Pf6N2E2_464at 529.775 kb on - strand, within Pf6N2E2_464at 530.732 kb on - strandat 530.754 kb on - strandat 530.890 kb on + strand, within Pf6N2E2_465at 530.905 kb on + strand, within Pf6N2E2_465at 530.905 kb on + strand, within Pf6N2E2_465at 530.905 kb on + strand, within Pf6N2E2_465at 530.906 kb on - strand, within Pf6N2E2_465at 530.906 kb on - strand, within Pf6N2E2_465at 530.965 kb on + strand, within Pf6N2E2_465at 530.965 kb on + strand, within Pf6N2E2_465at 530.965 kb on + strand, within Pf6N2E2_465at 530.965 kb on + strand, within Pf6N2E2_465at 530.966 kb on - strand, within Pf6N2E2_465at 530.968 kb on + strand, within Pf6N2E2_465at 530.968 kb on + strand, within Pf6N2E2_465at 530.969 kb on - strand, within Pf6N2E2_465at 530.969 kb on - strand, within Pf6N2E2_465at 531.239 kb on - strand, within Pf6N2E2_465at 531.239 kb on - strand, within Pf6N2E2_465at 531.239 kb on - strand, within Pf6N2E2_465at 531.401 kb on + strandat 531.401 kb on + strandat 531.401 kb on + strandat 531.401 kb on + strandat 531.401 kb on + strandat 531.404 kb on + strandat 531.404 kb on + strandat 531.405 kb on - strandat 531.405 kb on - strandat 531.434 kb on + strandat 531.435 kb on - strandat 531.435 kb on - strandat 531.615 kb on + strand, within Pf6N2E2_466at 531.615 kb on + strand, within Pf6N2E2_466at 531.616 kb on - strand, within Pf6N2E2_466

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3
remove
528,391 - +0.3
528,546 + +0.3
528,546 + -0.5
528,546 + -0.7
528,546 + +0.3
528,546 + +0.2
528,548 + -0.8
528,711 + -1.3
528,712 - -0.7
528,948 + Pf6N2E2_463 0.42 -0.7
528,948 + Pf6N2E2_463 0.42 +0.3
528,949 - Pf6N2E2_463 0.42 +0.3
528,949 - Pf6N2E2_463 0.42 +0.3
528,949 - Pf6N2E2_463 0.42 +1.9
528,949 - Pf6N2E2_463 0.42 +0.3
528,949 - Pf6N2E2_463 0.42 +0.3
528,995 + Pf6N2E2_463 0.49 +0.3
529,131 + Pf6N2E2_463 0.68 +0.3
529,132 - Pf6N2E2_463 0.68 -0.2
529,132 - Pf6N2E2_463 0.68 +0.3
529,171 + Pf6N2E2_463 0.74 -1.3
529,171 + Pf6N2E2_463 0.74 -0.6
529,172 - Pf6N2E2_463 0.74 +0.3
529,172 - Pf6N2E2_463 0.74 -0.7
529,172 - Pf6N2E2_463 0.74 +0.3
529,406 - +0.3
529,420 + -1.3
529,421 - +0.3
529,471 + -0.5
529,471 + +0.3
529,471 + +0.3
529,471 + -0.7
529,471 + +0.3
529,471 + +0.3
529,472 - +0.3
529,484 - +0.3
529,774 + Pf6N2E2_464 0.32 +0.3
529,775 - Pf6N2E2_464 0.32 +0.3
529,775 - Pf6N2E2_464 0.32 +0.3
530,732 - +0.3
530,754 - +0.3
530,890 + Pf6N2E2_465 0.25 -0.5
530,905 + Pf6N2E2_465 0.27 -0.7
530,905 + Pf6N2E2_465 0.27 -0.7
530,905 + Pf6N2E2_465 0.27 +0.3
530,906 - Pf6N2E2_465 0.27 -0.7
530,906 - Pf6N2E2_465 0.27 +0.3
530,965 + Pf6N2E2_465 0.37 +0.3
530,965 + Pf6N2E2_465 0.37 -0.7
530,965 + Pf6N2E2_465 0.37 -0.7
530,965 + Pf6N2E2_465 0.37 +0.3
530,966 - Pf6N2E2_465 0.37 +0.3
530,968 + Pf6N2E2_465 0.37 -0.7
530,968 + Pf6N2E2_465 0.37 +0.3
530,969 - Pf6N2E2_465 0.37 +0.3
530,969 - Pf6N2E2_465 0.37 +0.3
531,239 - Pf6N2E2_465 0.79 +0.3
531,239 - Pf6N2E2_465 0.79 -1.4
531,239 - Pf6N2E2_465 0.79 +0.3
531,401 + -1.1
531,401 + -1.5
531,401 + +0.3
531,401 + +0.3
531,401 + -0.7
531,404 + -0.5
531,404 + -0.7
531,405 - +1.5
531,405 - +0.3
531,434 + -1.3
531,435 - -0.7
531,435 - -0.8
531,615 + Pf6N2E2_466 0.31 +0.3
531,615 + Pf6N2E2_466 0.31 -0.5
531,616 - Pf6N2E2_466 0.31 +0.3

Or see this region's nucleotide sequence