Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_432

Experiment: Ying_Nucleobase14 rep C; time point 3

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Ying_Nucleobase14 rep C; time point 3
498,532	+		Pf6N2E2_430	0.78	-0.7
498,533	-		Pf6N2E2_430	0.78	+0.3
498,533	-		Pf6N2E2_430	0.78	-0.7
498,609	-		Pf6N2E2_430	0.85	-1.2
498,662	+		Pf6N2E2_430	0.90	-0.7
498,683	-				-1.1
498,809	-				+0.3
498,875	-		Pf6N2E2_431	0.15	-0.5
498,899	-		Pf6N2E2_431	0.18	+0.3
498,899	-		Pf6N2E2_431	0.18	+0.3
498,899	-		Pf6N2E2_431	0.18	+0.3
499,150	+		Pf6N2E2_431	0.55	-0.7
499,150	+		Pf6N2E2_431	0.55	+0.3
499,151	-		Pf6N2E2_431	0.55	-2.0
499,151	-		Pf6N2E2_431	0.55	-2.7
499,151	-		Pf6N2E2_431	0.55	-1.3
499,151	-		Pf6N2E2_431	0.55	-1.7
499,151	-		Pf6N2E2_431	0.55	-0.7
499,151	-		Pf6N2E2_431	0.55	+0.3
499,261	+		Pf6N2E2_431	0.72	-2.3
499,262	-		Pf6N2E2_431	0.72	-3.3
499,262	-		Pf6N2E2_431	0.72	-2.3
499,262	-		Pf6N2E2_431	0.72	+0.3
499,353	+		Pf6N2E2_431	0.85	-2.0
499,354	-		Pf6N2E2_431	0.85	+0.3
499,432	+				+0.3
499,433	-				-1.3
499,457	+				+0.3
499,511	+				-1.3
499,511	+				-0.7
499,512	-				-2.9
499,640	+		Pf6N2E2_432	0.24	-0.7
499,640	+		Pf6N2E2_432	0.24	-2.9
499,640	+		Pf6N2E2_432	0.24	-3.7
499,868	+		Pf6N2E2_432	0.55	-2.7
499,868	+		Pf6N2E2_432	0.55	-0.7
499,868	+		Pf6N2E2_432	0.55	+0.3
499,868	+		Pf6N2E2_432	0.55	+0.3
499,868	+		Pf6N2E2_432	0.55	-2.3
499,869	-		Pf6N2E2_432	0.55	+0.3
499,869	-		Pf6N2E2_432	0.55	+0.3
499,911	-		Pf6N2E2_432	0.60	-1.6
499,911	-		Pf6N2E2_432	0.60	-0.9
500,021	+		Pf6N2E2_432	0.75	-2.8
500,021	+		Pf6N2E2_432	0.75	-1.7
500,021	+		Pf6N2E2_432	0.75	+0.3
500,058	+		Pf6N2E2_432	0.80	-0.7
500,058	+		Pf6N2E2_432	0.80	-1.9
500,160	+				-0.7
500,160	+				-3.3
500,160	+				-3.0
500,161	-				-0.7
500,208	+				-1.3
500,208	+				+0.3
500,208	+				-3.3
500,208	+				-2.8
500,208	+				-0.7
500,208	+				+0.3
500,209	-				+0.3
500,209	-				-0.7
500,209	-				-0.7
500,209	-				+0.3
500,235	+				+0.3
500,235	+				+0.3
500,237	+				-0.7
500,238	-				-1.5
500,238	-				+0.3
500,240	-				+0.3
500,247	+				-0.5
500,248	-				-1.7
500,249	+				+0.3
500,249	+				+0.3
500,250	-				-0.5
500,250	-				+0.3
500,250	-				+0.3
500,251	+				+0.3
500,251	+				-0.7
500,252	-				+0.3
500,677	+		Pf6N2E2_433	0.32	-0.1
500,870	-		Pf6N2E2_433	0.50	-0.7
501,122	-		Pf6N2E2_433	0.73	-0.6
501,122	-		Pf6N2E2_433	0.73	-2.0
501,122	-		Pf6N2E2_433	0.73	-1.1
501,122	-		Pf6N2E2_433	0.73	-0.7