Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4005

Experiment: Ying_Nucleobase14 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4003 and Pf6N2E2_4004 are separated by 12 nucleotidesPf6N2E2_4004 and Pf6N2E2_4005 overlap by 12 nucleotidesPf6N2E2_4005 and Pf6N2E2_4006 are separated by 50 nucleotides Pf6N2E2_4003 - Sulfate transport system permease protein CysW, at 4,674,174 to 4,675,043 _4003 Pf6N2E2_4004 - Sulfate transport system permease protein CysT, at 4,675,056 to 4,675,775 _4004 Pf6N2E2_4005 - hypothetical protein, at 4,675,764 to 4,676,054 _4005 Pf6N2E2_4006 - Sulfate and thiosulfate binding protein CysP, at 4,676,105 to 4,677,118 _4006 Position (kb) 4675 4676 4677Strain fitness (log2 ratio) -2 -1 0 1at 4675.028 kb on + strandat 4675.154 kb on - strand, within Pf6N2E2_4004at 4675.159 kb on + strand, within Pf6N2E2_4004at 4675.160 kb on - strand, within Pf6N2E2_4004at 4675.160 kb on - strand, within Pf6N2E2_4004at 4675.243 kb on + strand, within Pf6N2E2_4004at 4675.244 kb on - strand, within Pf6N2E2_4004at 4675.465 kb on + strand, within Pf6N2E2_4004at 4675.465 kb on + strand, within Pf6N2E2_4004at 4675.465 kb on + strand, within Pf6N2E2_4004at 4675.466 kb on - strand, within Pf6N2E2_4004at 4675.522 kb on + strand, within Pf6N2E2_4004at 4675.522 kb on + strand, within Pf6N2E2_4004at 4675.541 kb on + strand, within Pf6N2E2_4004at 4675.541 kb on + strand, within Pf6N2E2_4004at 4675.542 kb on - strand, within Pf6N2E2_4004at 4675.615 kb on + strand, within Pf6N2E2_4004at 4675.615 kb on + strand, within Pf6N2E2_4004at 4675.615 kb on + strand, within Pf6N2E2_4004at 4675.620 kb on + strand, within Pf6N2E2_4004at 4675.621 kb on - strand, within Pf6N2E2_4004at 4675.672 kb on + strand, within Pf6N2E2_4004at 4675.673 kb on - strand, within Pf6N2E2_4004at 4675.788 kb on + strandat 4675.788 kb on + strandat 4675.810 kb on + strand, within Pf6N2E2_4005at 4675.810 kb on + strand, within Pf6N2E2_4005at 4675.874 kb on + strand, within Pf6N2E2_4005at 4675.874 kb on + strand, within Pf6N2E2_4005at 4675.874 kb on + strand, within Pf6N2E2_4005at 4675.874 kb on + strand, within Pf6N2E2_4005at 4675.874 kb on + strand, within Pf6N2E2_4005at 4675.875 kb on - strand, within Pf6N2E2_4005at 4675.875 kb on - strand, within Pf6N2E2_4005at 4675.875 kb on - strand, within Pf6N2E2_4005at 4675.875 kb on - strand, within Pf6N2E2_4005at 4675.875 kb on - strand, within Pf6N2E2_4005at 4676.057 kb on - strandat 4676.057 kb on - strandat 4676.091 kb on - strandat 4676.107 kb on - strandat 4676.107 kb on - strandat 4676.118 kb on + strandat 4676.118 kb on + strandat 4676.118 kb on + strandat 4676.118 kb on + strandat 4676.119 kb on + strandat 4676.119 kb on - strandat 4676.119 kb on - strandat 4676.229 kb on + strand, within Pf6N2E2_4006at 4676.229 kb on + strand, within Pf6N2E2_4006at 4676.229 kb on + strand, within Pf6N2E2_4006at 4676.263 kb on - strand, within Pf6N2E2_4006at 4676.301 kb on + strand, within Pf6N2E2_4006at 4676.301 kb on + strand, within Pf6N2E2_4006at 4676.301 kb on + strand, within Pf6N2E2_4006at 4676.301 kb on + strand, within Pf6N2E2_4006at 4676.302 kb on - strand, within Pf6N2E2_4006at 4676.302 kb on - strand, within Pf6N2E2_4006at 4676.316 kb on + strand, within Pf6N2E2_4006at 4676.317 kb on - strand, within Pf6N2E2_4006at 4676.509 kb on + strand, within Pf6N2E2_4006at 4676.510 kb on - strand, within Pf6N2E2_4006at 4676.510 kb on - strand, within Pf6N2E2_4006at 4676.571 kb on + strand, within Pf6N2E2_4006at 4676.572 kb on - strand, within Pf6N2E2_4006at 4676.616 kb on + strand, within Pf6N2E2_4006at 4676.766 kb on + strand, within Pf6N2E2_4006at 4676.766 kb on + strand, within Pf6N2E2_4006at 4676.766 kb on + strand, within Pf6N2E2_4006at 4676.767 kb on - strand, within Pf6N2E2_4006at 4676.767 kb on - strand, within Pf6N2E2_4006at 4676.767 kb on - strand, within Pf6N2E2_4006at 4676.767 kb on - strand, within Pf6N2E2_4006at 4676.773 kb on + strand, within Pf6N2E2_4006at 4676.773 kb on + strand, within Pf6N2E2_4006at 4676.773 kb on + strand, within Pf6N2E2_4006at 4676.773 kb on + strand, within Pf6N2E2_4006at 4676.773 kb on + strand, within Pf6N2E2_4006at 4676.774 kb on - strand, within Pf6N2E2_4006at 4676.991 kb on + strand, within Pf6N2E2_4006at 4676.991 kb on + strand, within Pf6N2E2_4006at 4676.991 kb on + strand, within Pf6N2E2_4006at 4676.991 kb on + strand, within Pf6N2E2_4006at 4676.991 kb on + strand, within Pf6N2E2_4006at 4676.991 kb on + strand, within Pf6N2E2_4006at 4676.992 kb on - strand, within Pf6N2E2_4006at 4676.992 kb on - strand, within Pf6N2E2_4006at 4676.992 kb on - strand, within Pf6N2E2_4006at 4676.992 kb on - strand, within Pf6N2E2_4006at 4676.992 kb on - strand, within Pf6N2E2_4006at 4677.021 kb on + strandat 4677.021 kb on + strandat 4677.022 kb on - strandat 4677.042 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3
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4,675,028 + +0.5
4,675,154 - Pf6N2E2_4004 0.14 +0.5
4,675,159 + Pf6N2E2_4004 0.14 -0.5
4,675,160 - Pf6N2E2_4004 0.14 +0.5
4,675,160 - Pf6N2E2_4004 0.14 +0.5
4,675,243 + Pf6N2E2_4004 0.26 -0.6
4,675,244 - Pf6N2E2_4004 0.26 +0.5
4,675,465 + Pf6N2E2_4004 0.57 +0.3
4,675,465 + Pf6N2E2_4004 0.57 -0.7
4,675,465 + Pf6N2E2_4004 0.57 -0.5
4,675,466 - Pf6N2E2_4004 0.57 -0.2
4,675,522 + Pf6N2E2_4004 0.65 -2.1
4,675,522 + Pf6N2E2_4004 0.65 -0.5
4,675,541 + Pf6N2E2_4004 0.67 -0.5
4,675,541 + Pf6N2E2_4004 0.67 -0.5
4,675,542 - Pf6N2E2_4004 0.68 +0.5
4,675,615 + Pf6N2E2_4004 0.78 -0.1
4,675,615 + Pf6N2E2_4004 0.78 -1.3
4,675,615 + Pf6N2E2_4004 0.78 -0.5
4,675,620 + Pf6N2E2_4004 0.78 -1.1
4,675,621 - Pf6N2E2_4004 0.78 -0.7
4,675,672 + Pf6N2E2_4004 0.86 +0.1
4,675,673 - Pf6N2E2_4004 0.86 -0.5
4,675,788 + +0.5
4,675,788 + +0.5
4,675,810 + Pf6N2E2_4005 0.16 -0.5
4,675,810 + Pf6N2E2_4005 0.16 -2.3
4,675,874 + Pf6N2E2_4005 0.38 -1.5
4,675,874 + Pf6N2E2_4005 0.38 -1.1
4,675,874 + Pf6N2E2_4005 0.38 -1.2
4,675,874 + Pf6N2E2_4005 0.38 -0.5
4,675,874 + Pf6N2E2_4005 0.38 +0.5
4,675,875 - Pf6N2E2_4005 0.38 -0.5
4,675,875 - Pf6N2E2_4005 0.38 +0.5
4,675,875 - Pf6N2E2_4005 0.38 -0.5
4,675,875 - Pf6N2E2_4005 0.38 -1.1
4,675,875 - Pf6N2E2_4005 0.38 -1.8
4,676,057 - -0.6
4,676,057 - +0.5
4,676,091 - +0.5
4,676,107 - +0.8
4,676,107 - +0.5
4,676,118 + -0.5
4,676,118 + +0.5
4,676,118 + -0.5
4,676,118 + +0.5
4,676,119 + -0.5
4,676,119 - +0.5
4,676,119 - +0.5
4,676,229 + Pf6N2E2_4006 0.12 -0.5
4,676,229 + Pf6N2E2_4006 0.12 +0.5
4,676,229 + Pf6N2E2_4006 0.12 -1.8
4,676,263 - Pf6N2E2_4006 0.16 -1.2
4,676,301 + Pf6N2E2_4006 0.19 -0.7
4,676,301 + Pf6N2E2_4006 0.19 -0.8
4,676,301 + Pf6N2E2_4006 0.19 -0.8
4,676,301 + Pf6N2E2_4006 0.19 -0.9
4,676,302 - Pf6N2E2_4006 0.19 -0.8
4,676,302 - Pf6N2E2_4006 0.19 +0.5
4,676,316 + Pf6N2E2_4006 0.21 -0.5
4,676,317 - Pf6N2E2_4006 0.21 -0.5
4,676,509 + Pf6N2E2_4006 0.40 -0.8
4,676,510 - Pf6N2E2_4006 0.40 -0.5
4,676,510 - Pf6N2E2_4006 0.40 +0.5
4,676,571 + Pf6N2E2_4006 0.46 -0.5
4,676,572 - Pf6N2E2_4006 0.46 +0.5
4,676,616 + Pf6N2E2_4006 0.50 +0.5
4,676,766 + Pf6N2E2_4006 0.65 +0.5
4,676,766 + Pf6N2E2_4006 0.65 +0.5
4,676,766 + Pf6N2E2_4006 0.65 +0.5
4,676,767 - Pf6N2E2_4006 0.65 -0.5
4,676,767 - Pf6N2E2_4006 0.65 -0.5
4,676,767 - Pf6N2E2_4006 0.65 -1.5
4,676,767 - Pf6N2E2_4006 0.65 -0.5
4,676,773 + Pf6N2E2_4006 0.66 +0.5
4,676,773 + Pf6N2E2_4006 0.66 +0.5
4,676,773 + Pf6N2E2_4006 0.66 -0.5
4,676,773 + Pf6N2E2_4006 0.66 -0.5
4,676,773 + Pf6N2E2_4006 0.66 -1.1
4,676,774 - Pf6N2E2_4006 0.66 -0.6
4,676,991 + Pf6N2E2_4006 0.87 -0.5
4,676,991 + Pf6N2E2_4006 0.87 -1.9
4,676,991 + Pf6N2E2_4006 0.87 +1.3
4,676,991 + Pf6N2E2_4006 0.87 -1.6
4,676,991 + Pf6N2E2_4006 0.87 -0.5
4,676,991 + Pf6N2E2_4006 0.87 +1.5
4,676,992 - Pf6N2E2_4006 0.87 +0.5
4,676,992 - Pf6N2E2_4006 0.87 -1.1
4,676,992 - Pf6N2E2_4006 0.87 -0.5
4,676,992 - Pf6N2E2_4006 0.87 -0.5
4,676,992 - Pf6N2E2_4006 0.87 -0.5
4,677,021 + -1.5
4,677,021 + +0.5
4,677,022 - -1.5
4,677,042 + -1.5

Or see this region's nucleotide sequence