Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_313 and Pf6N2E2_314 are separated by 40 nucleotides Pf6N2E2_314 and Pf6N2E2_315 are separated by 193 nucleotides
Pf6N2E2_313 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 379,362 to 381,089
_313
Pf6N2E2_314 - Isochorismatase (EC 3.3.2.1), at 381,130 to 381,663
_314
Pf6N2E2_315 - probable transmembrane protein, at 381,857 to 382,717
_315
Position (kb) 381
382 Strain fitness (log2 ratio) -2
-1
0
1 at 380.163 kb on + strand, within Pf6N2E2_313 at 380.292 kb on + strand, within Pf6N2E2_313 at 380.320 kb on - strand, within Pf6N2E2_313 at 380.337 kb on + strand, within Pf6N2E2_313 at 380.337 kb on + strand, within Pf6N2E2_313 at 380.338 kb on - strand, within Pf6N2E2_313 at 380.387 kb on + strand, within Pf6N2E2_313 at 380.497 kb on - strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.538 kb on + strand, within Pf6N2E2_313 at 380.539 kb on - strand, within Pf6N2E2_313 at 380.539 kb on - strand, within Pf6N2E2_313 at 380.646 kb on + strand, within Pf6N2E2_313 at 380.647 kb on - strand, within Pf6N2E2_313 at 380.647 kb on - strand, within Pf6N2E2_313 at 380.799 kb on - strand, within Pf6N2E2_313 at 380.817 kb on + strand, within Pf6N2E2_313 at 381.039 kb on + strand at 381.039 kb on + strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.040 kb on - strand at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.304 kb on + strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.305 kb on - strand, within Pf6N2E2_314 at 381.392 kb on - strand, within Pf6N2E2_314 at 381.661 kb on + strand at 381.682 kb on - strand at 381.682 kb on - strand at 381.691 kb on + strand at 381.885 kb on + strand at 381.885 kb on + strand at 381.886 kb on - strand at 381.886 kb on - strand at 382.042 kb on + strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.043 kb on - strand, within Pf6N2E2_315 at 382.262 kb on - strand, within Pf6N2E2_315 at 382.359 kb on + strand, within Pf6N2E2_315 at 382.360 kb on - strand, within Pf6N2E2_315 at 382.360 kb on - strand, within Pf6N2E2_315 at 382.655 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 380,163 + Pf6N2E2_313 0.46 -1.7 380,292 + Pf6N2E2_313 0.54 -2.3 380,320 - Pf6N2E2_313 0.55 -0.5 380,337 + Pf6N2E2_313 0.56 -0.3 380,337 + Pf6N2E2_313 0.56 -1.7 380,338 - Pf6N2E2_313 0.56 -1.0 380,387 + Pf6N2E2_313 0.59 -1.3 380,497 - Pf6N2E2_313 0.66 -0.3 380,538 + Pf6N2E2_313 0.68 +0.3 380,538 + Pf6N2E2_313 0.68 -0.8 380,538 + Pf6N2E2_313 0.68 -0.6 380,538 + Pf6N2E2_313 0.68 -0.3 380,539 - Pf6N2E2_313 0.68 +0.3 380,539 - Pf6N2E2_313 0.68 -0.7 380,646 + Pf6N2E2_313 0.74 +0.3 380,647 - Pf6N2E2_313 0.74 -0.9 380,647 - Pf6N2E2_313 0.74 -2.6 380,799 - Pf6N2E2_313 0.83 +0.3 380,817 + Pf6N2E2_313 0.84 +0.3 381,039 + -0.7 381,039 + -0.7 381,040 - -0.7 381,040 - +0.3 381,040 - +0.3 381,040 - -0.7 381,040 - -0.7 381,304 + Pf6N2E2_314 0.33 -0.7 381,304 + Pf6N2E2_314 0.33 +0.3 381,304 + Pf6N2E2_314 0.33 -0.7 381,304 + Pf6N2E2_314 0.33 +0.3 381,304 + Pf6N2E2_314 0.33 -2.1 381,305 - Pf6N2E2_314 0.33 +0.3 381,305 - Pf6N2E2_314 0.33 +0.3 381,305 - Pf6N2E2_314 0.33 +0.3 381,305 - Pf6N2E2_314 0.33 +0.3 381,392 - Pf6N2E2_314 0.49 +0.3 381,661 + -1.1 381,682 - -1.3 381,682 - -1.3 381,691 + +0.3 381,885 + +0.3 381,885 + +0.3 381,886 - +0.3 381,886 - +1.1 382,042 + Pf6N2E2_315 0.21 +0.3 382,043 - Pf6N2E2_315 0.22 +0.3 382,043 - Pf6N2E2_315 0.22 +0.3 382,043 - Pf6N2E2_315 0.22 -1.3 382,262 - Pf6N2E2_315 0.47 +0.3 382,359 + Pf6N2E2_315 0.58 -0.8 382,360 - Pf6N2E2_315 0.58 -1.3 382,360 - Pf6N2E2_315 0.58 -0.9 382,655 + +0.3
Or see this region's nucleotide sequence