Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_238 and Pf6N2E2_239 are separated by 33 nucleotides Pf6N2E2_239 and Pf6N2E2_240 are separated by 171 nucleotides Pf6N2E2_240 and Pf6N2E2_241 are separated by 482 nucleotides Pf6N2E2_241 and Pf6N2E2_242 overlap by 1 nucleotides
Pf6N2E2_238 - hypothetical protein, at 299,977 to 300,096
_238
Pf6N2E2_239 - Fatty acid desaturase, at 300,130 to 300,831
_239
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Pf6N2E2_241 - hypothetical protein, at 302,376 to 302,519
_241
Pf6N2E2_242 - Glycine betaine ABC transport system permease protein, at 302,519 to 303,202
_242
Position (kb) 301
302 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 300.010 kb on - strand, within Pf6N2E2_238 at 300.172 kb on + strand at 300.183 kb on + strand at 300.184 kb on - strand at 300.184 kb on - strand at 300.295 kb on + strand, within Pf6N2E2_239 at 300.295 kb on + strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.559 kb on + strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.709 kb on - strand, within Pf6N2E2_239 at 300.749 kb on - strand, within Pf6N2E2_239 at 300.764 kb on - strand at 300.764 kb on - strand at 300.773 kb on + strand at 300.830 kb on - strand at 300.835 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.845 kb on - strand at 300.845 kb on - strand at 300.894 kb on + strand at 300.894 kb on + strand at 300.960 kb on - strand at 300.960 kb on - strand at 301.124 kb on + strand, within Pf6N2E2_240 at 301.124 kb on + strand, within Pf6N2E2_240 at 301.125 kb on - strand, within Pf6N2E2_240 at 301.191 kb on + strand, within Pf6N2E2_240 at 301.191 kb on + strand, within Pf6N2E2_240 at 301.191 kb on + strand, within Pf6N2E2_240 at 301.258 kb on + strand, within Pf6N2E2_240 at 301.258 kb on + strand, within Pf6N2E2_240 at 301.258 kb on + strand, within Pf6N2E2_240 at 301.334 kb on + strand, within Pf6N2E2_240 at 301.334 kb on + strand, within Pf6N2E2_240 at 301.335 kb on - strand, within Pf6N2E2_240 at 301.423 kb on - strand, within Pf6N2E2_240 at 301.641 kb on + strand, within Pf6N2E2_240 at 301.642 kb on - strand, within Pf6N2E2_240 at 301.952 kb on + strand at 301.981 kb on + strand at 302.115 kb on - strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.244 kb on + strand at 302.336 kb on - strand at 302.481 kb on + strand, within Pf6N2E2_241 at 302.540 kb on + strand at 302.540 kb on + strand at 302.612 kb on + strand, within Pf6N2E2_242 at 302.677 kb on + strand, within Pf6N2E2_242 at 302.682 kb on - strand, within Pf6N2E2_242 at 302.840 kb on - strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 300,010 - Pf6N2E2_238 0.28 +0.3 300,172 + +0.3 300,183 + +0.3 300,184 - +0.3 300,184 - +0.3 300,295 + Pf6N2E2_239 0.24 -0.7 300,295 + Pf6N2E2_239 0.24 +0.3 300,296 - Pf6N2E2_239 0.24 +0.3 300,296 - Pf6N2E2_239 0.24 -0.8 300,297 + Pf6N2E2_239 0.24 +0.3 300,297 + Pf6N2E2_239 0.24 +0.3 300,297 + Pf6N2E2_239 0.24 -0.7 300,298 - Pf6N2E2_239 0.24 +0.1 300,298 - Pf6N2E2_239 0.24 +0.3 300,298 - Pf6N2E2_239 0.24 +0.3 300,298 - Pf6N2E2_239 0.24 +0.1 300,298 - Pf6N2E2_239 0.24 -0.7 300,298 - Pf6N2E2_239 0.24 +0.3 300,298 - Pf6N2E2_239 0.24 -0.7 300,482 - Pf6N2E2_239 0.50 -1.1 300,482 - Pf6N2E2_239 0.50 -3.3 300,506 - Pf6N2E2_239 0.54 -0.7 300,506 - Pf6N2E2_239 0.54 -1.3 300,559 + Pf6N2E2_239 0.61 +0.3 300,595 - Pf6N2E2_239 0.66 -0.9 300,595 - Pf6N2E2_239 0.66 +0.3 300,707 - Pf6N2E2_239 0.82 -1.7 300,707 - Pf6N2E2_239 0.82 +0.3 300,709 - Pf6N2E2_239 0.82 +0.3 300,749 - Pf6N2E2_239 0.88 +0.3 300,764 - -0.7 300,764 - +0.3 300,773 + +0.3 300,830 - +0.3 300,835 + +0.3 300,844 + +0.3 300,844 + +0.3 300,844 + -0.9 300,844 + -0.7 300,845 - +0.3 300,845 - -0.4 300,894 + -0.8 300,894 + +0.3 300,960 - +0.3 300,960 - -1.1 301,124 + Pf6N2E2_240 0.14 +0.3 301,124 + Pf6N2E2_240 0.14 +0.0 301,125 - Pf6N2E2_240 0.14 -0.7 301,191 + Pf6N2E2_240 0.21 +0.5 301,191 + Pf6N2E2_240 0.21 +0.3 301,191 + Pf6N2E2_240 0.21 -0.3 301,258 + Pf6N2E2_240 0.29 +0.3 301,258 + Pf6N2E2_240 0.29 -0.7 301,258 + Pf6N2E2_240 0.29 -0.3 301,334 + Pf6N2E2_240 0.37 +0.3 301,334 + Pf6N2E2_240 0.37 +0.3 301,335 - Pf6N2E2_240 0.37 -0.7 301,423 - Pf6N2E2_240 0.47 -0.7 301,641 + Pf6N2E2_240 0.72 +0.5 301,642 - Pf6N2E2_240 0.72 +0.3 301,952 + -1.0 301,981 + +0.3 302,115 - +0.3 302,157 + +0.3 302,157 + +0.3 302,157 + -1.7 302,244 + -0.5 302,336 - +0.3 302,481 + Pf6N2E2_241 0.73 +0.3 302,540 + -0.7 302,540 + +0.3 302,612 + Pf6N2E2_242 0.14 +0.3 302,677 + Pf6N2E2_242 0.23 +0.3 302,682 - Pf6N2E2_242 0.24 +0.1 302,840 - Pf6N2E2_242 0.47 +0.3 302,843 + Pf6N2E2_242 0.47 -1.7 302,843 + Pf6N2E2_242 0.47 -0.5 302,843 + Pf6N2E2_242 0.47 -0.7 302,843 + Pf6N2E2_242 0.47 -0.7 302,843 + Pf6N2E2_242 0.47 +0.3 302,843 + Pf6N2E2_242 0.47 +0.3 302,843 + Pf6N2E2_242 0.47 +0.3 302,843 + Pf6N2E2_242 0.47 -1.6 302,844 - Pf6N2E2_242 0.48 -0.7 302,844 - Pf6N2E2_242 0.48 +0.3
Or see this region's nucleotide sequence