Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2358 and Pf6N2E2_2359 are separated by 10 nucleotides Pf6N2E2_2359 and Pf6N2E2_2360 are separated by 255 nucleotides
Pf6N2E2_2358 - Type III secretion host injection protein (YopB), at 2,792,123 to 2,793,223
_2358
Pf6N2E2_2359 - hypothetical protein, at 2,793,234 to 2,794,343
_2359
Pf6N2E2_2360 - cell invasion protein SipB, at 2,794,599 to 2,796,335
_2360
Position (kb)
2793
2794
2795 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2792.296 kb on - strand, within Pf6N2E2_2358 at 2792.345 kb on + strand, within Pf6N2E2_2358 at 2792.345 kb on + strand, within Pf6N2E2_2358 at 2792.468 kb on - strand, within Pf6N2E2_2358 at 2792.504 kb on - strand, within Pf6N2E2_2358 at 2792.542 kb on + strand, within Pf6N2E2_2358 at 2792.543 kb on - strand, within Pf6N2E2_2358 at 2792.543 kb on - strand, within Pf6N2E2_2358 at 2792.559 kb on - strand, within Pf6N2E2_2358 at 2792.630 kb on - strand, within Pf6N2E2_2358 at 2792.633 kb on + strand, within Pf6N2E2_2358 at 2792.634 kb on - strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.698 kb on - strand, within Pf6N2E2_2358 at 2793.126 kb on - strand at 2793.126 kb on - strand at 2793.204 kb on - strand at 2793.410 kb on - strand, within Pf6N2E2_2359 at 2793.561 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2794.112 kb on + strand at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359 at 2794.261 kb on - strand at 2794.589 kb on + strand at 2794.589 kb on + strand at 2794.589 kb on + strand at 2794.589 kb on + strand at 2794.590 kb on - strand at 2794.590 kb on - strand at 2794.590 kb on - strand at 2794.593 kb on + strand at 2794.594 kb on - strand at 2794.601 kb on - strand at 2794.735 kb on + strand at 2794.736 kb on - strand at 2795.210 kb on - strand, within Pf6N2E2_2360 at 2795.305 kb on - strand, within Pf6N2E2_2360
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 2,792,296 - Pf6N2E2_2358 0.16 +0.3 2,792,345 + Pf6N2E2_2358 0.20 +0.3 2,792,345 + Pf6N2E2_2358 0.20 +0.3 2,792,468 - Pf6N2E2_2358 0.31 +0.3 2,792,504 - Pf6N2E2_2358 0.35 +0.3 2,792,542 + Pf6N2E2_2358 0.38 -1.3 2,792,543 - Pf6N2E2_2358 0.38 +0.3 2,792,543 - Pf6N2E2_2358 0.38 +0.3 2,792,559 - Pf6N2E2_2358 0.40 -0.7 2,792,630 - Pf6N2E2_2358 0.46 -0.3 2,792,633 + Pf6N2E2_2358 0.46 -0.7 2,792,634 - Pf6N2E2_2358 0.46 +0.3 2,792,664 + Pf6N2E2_2358 0.49 -0.4 2,792,664 + Pf6N2E2_2358 0.49 -0.3 2,792,664 + Pf6N2E2_2358 0.49 +0.3 2,792,664 + Pf6N2E2_2358 0.49 -0.5 2,792,664 + Pf6N2E2_2358 0.49 +0.3 2,792,665 - Pf6N2E2_2358 0.49 +0.3 2,792,665 - Pf6N2E2_2358 0.49 +0.3 2,792,665 - Pf6N2E2_2358 0.49 -1.3 2,792,665 - Pf6N2E2_2358 0.49 -0.7 2,792,665 - Pf6N2E2_2358 0.49 +0.3 2,792,665 - Pf6N2E2_2358 0.49 +0.3 2,792,698 - Pf6N2E2_2358 0.52 +1.9 2,793,126 - +0.3 2,793,126 - +0.3 2,793,204 - -0.8 2,793,410 - Pf6N2E2_2359 0.16 +0.3 2,793,561 + Pf6N2E2_2359 0.29 -0.7 2,793,667 + Pf6N2E2_2359 0.39 -0.7 2,793,667 + Pf6N2E2_2359 0.39 +0.3 2,793,667 + Pf6N2E2_2359 0.39 -1.7 2,793,667 + Pf6N2E2_2359 0.39 -0.7 2,793,668 - Pf6N2E2_2359 0.39 +0.3 2,793,668 - Pf6N2E2_2359 0.39 +0.3 2,793,934 + Pf6N2E2_2359 0.63 +0.3 2,793,934 + Pf6N2E2_2359 0.63 -0.7 2,793,934 + Pf6N2E2_2359 0.63 -1.7 2,794,112 + -0.7 2,794,115 + Pf6N2E2_2359 0.79 +0.3 2,794,115 + Pf6N2E2_2359 0.79 +0.3 2,794,115 + Pf6N2E2_2359 0.79 -2.1 2,794,115 + Pf6N2E2_2359 0.79 -1.5 2,794,116 - Pf6N2E2_2359 0.79 +0.3 2,794,116 - Pf6N2E2_2359 0.79 +0.3 2,794,261 - +0.3 2,794,589 + +0.3 2,794,589 + +0.3 2,794,589 + +0.3 2,794,589 + -0.7 2,794,590 - +0.3 2,794,590 - -0.9 2,794,590 - +0.3 2,794,593 + +0.3 2,794,594 - -1.7 2,794,601 - +0.3 2,794,735 + +0.3 2,794,736 - +0.3 2,795,210 - Pf6N2E2_2360 0.35 -0.7 2,795,305 - Pf6N2E2_2360 0.41 +0.3
Or see this region's nucleotide sequence