Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_161 and Pf6N2E2_162 are separated by 58 nucleotides Pf6N2E2_162 and Pf6N2E2_163 overlap by 4 nucleotides Pf6N2E2_163 and Pf6N2E2_164 are separated by 3 nucleotides
Pf6N2E2_161 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 214,281 to 215,237
_161
Pf6N2E2_162 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 215,296 to 216,849
_162
Pf6N2E2_163 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 216,846 to 217,823
_163
Pf6N2E2_164 - Ribose operon repressor, at 217,827 to 218,846
_164
Position (kb)
215
216
217 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.456 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 215.235 kb on + strand at 215.235 kb on + strand at 215.235 kb on + strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.240 kb on - strand at 215.240 kb on - strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.296 kb on - strand at 215.350 kb on + strand at 215.350 kb on + strand at 215.351 kb on - strand at 215.351 kb on - strand at 215.351 kb on - strand at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 216.087 kb on + strand, within Pf6N2E2_162 at 216.156 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.888 kb on + strand at 216.889 kb on - strand at 216.903 kb on + strand at 216.904 kb on - strand at 216.975 kb on + strand, within Pf6N2E2_163 at 216.975 kb on + strand, within Pf6N2E2_163 at 216.990 kb on - strand, within Pf6N2E2_163 at 217.302 kb on + strand, within Pf6N2E2_163 at 217.302 kb on + strand, within Pf6N2E2_163 at 217.303 kb on - strand, within Pf6N2E2_163 at 217.303 kb on - strand, within Pf6N2E2_163 at 217.492 kb on - strand, within Pf6N2E2_163 at 217.505 kb on - strand, within Pf6N2E2_163 at 217.631 kb on + strand, within Pf6N2E2_163 at 217.631 kb on + strand, within Pf6N2E2_163 at 217.791 kb on + strand at 217.792 kb on - strand at 217.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 214,455 + Pf6N2E2_161 0.18 +0.4 214,455 + Pf6N2E2_161 0.18 +0.4 214,455 + Pf6N2E2_161 0.18 +0.4 214,456 - Pf6N2E2_161 0.18 -0.6 214,753 - Pf6N2E2_161 0.49 +0.4 214,753 - Pf6N2E2_161 0.49 +0.4 215,235 + +0.4 215,235 + -1.1 215,235 + +0.4 215,236 - +0.4 215,236 - -0.6 215,236 - -4.4 215,236 - -2.4 215,240 - -3.8 215,240 - -3.2 215,295 + -0.6 215,295 + +0.4 215,295 + -3.0 215,295 + -2.3 215,295 + -0.6 215,295 + -2.6 215,295 + -3.2 215,295 + +0.4 215,295 + -3.4 215,296 - -0.6 215,350 + -2.0 215,350 + -3.0 215,351 - -5.9 215,351 - -0.6 215,351 - -3.6 215,893 + Pf6N2E2_162 0.38 +0.4 215,893 + Pf6N2E2_162 0.38 -0.6 215,893 + Pf6N2E2_162 0.38 -2.8 215,893 + Pf6N2E2_162 0.38 -1.2 215,893 + Pf6N2E2_162 0.38 -2.4 215,893 + Pf6N2E2_162 0.38 -1.2 215,894 - Pf6N2E2_162 0.38 -0.6 215,894 - Pf6N2E2_162 0.38 +0.4 215,894 - Pf6N2E2_162 0.38 +0.4 215,894 - Pf6N2E2_162 0.38 -1.0 215,894 - Pf6N2E2_162 0.38 -0.6 215,989 + Pf6N2E2_162 0.45 +0.4 215,989 + Pf6N2E2_162 0.45 -4.2 215,989 + Pf6N2E2_162 0.45 +0.4 215,989 + Pf6N2E2_162 0.45 -3.0 215,990 - Pf6N2E2_162 0.45 -2.0 215,990 - Pf6N2E2_162 0.45 -1.6 215,990 - Pf6N2E2_162 0.45 -0.6 216,087 + Pf6N2E2_162 0.51 -0.6 216,156 + Pf6N2E2_162 0.55 -5.7 216,616 + Pf6N2E2_162 0.85 +0.4 216,616 + Pf6N2E2_162 0.85 +0.4 216,616 + Pf6N2E2_162 0.85 -3.6 216,616 + Pf6N2E2_162 0.85 -3.2 216,617 - Pf6N2E2_162 0.85 +0.4 216,617 - Pf6N2E2_162 0.85 -1.2 216,617 - Pf6N2E2_162 0.85 -3.7 216,617 - Pf6N2E2_162 0.85 +0.4 216,888 + +0.4 216,889 - -0.6 216,903 + +0.4 216,904 - -0.6 216,975 + Pf6N2E2_163 0.13 +0.4 216,975 + Pf6N2E2_163 0.13 +0.4 216,990 - Pf6N2E2_163 0.15 +0.4 217,302 + Pf6N2E2_163 0.47 +0.4 217,302 + Pf6N2E2_163 0.47 +0.4 217,303 - Pf6N2E2_163 0.47 +0.4 217,303 - Pf6N2E2_163 0.47 +0.4 217,492 - Pf6N2E2_163 0.66 -5.9 217,505 - Pf6N2E2_163 0.67 -2.0 217,631 + Pf6N2E2_163 0.80 -0.6 217,631 + Pf6N2E2_163 0.80 -2.0 217,791 + +0.4 217,792 - +0.4 217,847 - +0.4
Or see this region's nucleotide sequence