Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1598 and Pf6N2E2_1599 are separated by 9 nucleotides Pf6N2E2_1599 and Pf6N2E2_1600 are separated by 130 nucleotides Pf6N2E2_1600 and Pf6N2E2_1601 are separated by 5 nucleotides Pf6N2E2_1601 and Pf6N2E2_1602 are separated by 70 nucleotides
Pf6N2E2_1598 - Nitrate reductase cytochrome c550-type subunit, at 1,870,998 to 1,871,483
_1598
Pf6N2E2_1599 - Cytochrome c-type protein NapC, at 1,871,493 to 1,872,089
_1599
Pf6N2E2_1600 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 1,872,220 to 1,873,872
_1600
Pf6N2E2_1601 - DNA-binding response regulator, LuxR family, at 1,873,878 to 1,874,459
_1601
Pf6N2E2_1602 - hypothetical protein, at 1,874,530 to 1,875,312
_1602
Position (kb)
1872
1873
1874 Strain fitness (log2 ratio)
-2
-1
0
1 at 1871.319 kb on + strand, within Pf6N2E2_1598 at 1871.320 kb on - strand, within Pf6N2E2_1598 at 1871.368 kb on - strand, within Pf6N2E2_1598 at 1871.404 kb on + strand, within Pf6N2E2_1598 at 1871.404 kb on + strand, within Pf6N2E2_1598 at 1871.405 kb on - strand, within Pf6N2E2_1598 at 1871.405 kb on - strand, within Pf6N2E2_1598 at 1871.524 kb on - strand at 1871.553 kb on + strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.557 kb on - strand, within Pf6N2E2_1599 at 1871.557 kb on - strand, within Pf6N2E2_1599 at 1871.715 kb on + strand, within Pf6N2E2_1599 at 1871.781 kb on - strand, within Pf6N2E2_1599 at 1872.224 kb on - strand at 1872.224 kb on - strand at 1872.224 kb on - strand at 1872.224 kb on - strand at 1872.370 kb on + strand at 1872.370 kb on + strand at 1872.524 kb on - strand, within Pf6N2E2_1600 at 1872.524 kb on - strand, within Pf6N2E2_1600 at 1872.982 kb on + strand, within Pf6N2E2_1600 at 1873.103 kb on + strand, within Pf6N2E2_1600 at 1873.103 kb on + strand, within Pf6N2E2_1600 at 1873.104 kb on - strand, within Pf6N2E2_1600 at 1873.229 kb on + strand, within Pf6N2E2_1600 at 1873.397 kb on - strand, within Pf6N2E2_1600 at 1873.514 kb on + strand, within Pf6N2E2_1600 at 1873.595 kb on + strand, within Pf6N2E2_1600 at 1873.595 kb on + strand, within Pf6N2E2_1600 at 1873.596 kb on - strand, within Pf6N2E2_1600 at 1873.889 kb on + strand at 1873.890 kb on - strand at 1874.285 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.343 kb on - strand, within Pf6N2E2_1601 at 1874.391 kb on + strand, within Pf6N2E2_1601 at 1874.391 kb on + strand, within Pf6N2E2_1601 at 1874.392 kb on - strand, within Pf6N2E2_1601 at 1874.508 kb on - strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.831 kb on + strand, within Pf6N2E2_1602
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 1,871,319 + Pf6N2E2_1598 0.66 -1.3 1,871,320 - Pf6N2E2_1598 0.66 +0.3 1,871,368 - Pf6N2E2_1598 0.76 +0.3 1,871,404 + Pf6N2E2_1598 0.84 +0.3 1,871,404 + Pf6N2E2_1598 0.84 -1.3 1,871,405 - Pf6N2E2_1598 0.84 +0.3 1,871,405 - Pf6N2E2_1598 0.84 -0.7 1,871,524 - -1.2 1,871,553 + Pf6N2E2_1599 0.10 -0.7 1,871,554 - Pf6N2E2_1599 0.10 -0.7 1,871,554 - Pf6N2E2_1599 0.10 -0.6 1,871,554 - Pf6N2E2_1599 0.10 +0.3 1,871,554 - Pf6N2E2_1599 0.10 +0.3 1,871,554 - Pf6N2E2_1599 0.10 -1.0 1,871,554 - Pf6N2E2_1599 0.10 +0.3 1,871,554 - Pf6N2E2_1599 0.10 +0.3 1,871,557 - Pf6N2E2_1599 0.11 +0.3 1,871,557 - Pf6N2E2_1599 0.11 -0.7 1,871,715 + Pf6N2E2_1599 0.37 +0.3 1,871,781 - Pf6N2E2_1599 0.48 +0.3 1,872,224 - -0.7 1,872,224 - +0.3 1,872,224 - -0.7 1,872,224 - +0.3 1,872,370 + +0.3 1,872,370 + -0.7 1,872,524 - Pf6N2E2_1600 0.18 -0.7 1,872,524 - Pf6N2E2_1600 0.18 -0.9 1,872,982 + Pf6N2E2_1600 0.46 -2.6 1,873,103 + Pf6N2E2_1600 0.53 -0.8 1,873,103 + Pf6N2E2_1600 0.53 +0.3 1,873,104 - Pf6N2E2_1600 0.53 -0.7 1,873,229 + Pf6N2E2_1600 0.61 -0.7 1,873,397 - Pf6N2E2_1600 0.71 +0.3 1,873,514 + Pf6N2E2_1600 0.78 +0.3 1,873,595 + Pf6N2E2_1600 0.83 +0.3 1,873,595 + Pf6N2E2_1600 0.83 -1.6 1,873,596 - Pf6N2E2_1600 0.83 +0.3 1,873,889 + +0.3 1,873,890 - +0.3 1,874,285 + Pf6N2E2_1601 0.70 +0.3 1,874,340 + Pf6N2E2_1601 0.79 +0.3 1,874,340 + Pf6N2E2_1601 0.79 +0.3 1,874,340 + Pf6N2E2_1601 0.79 +0.3 1,874,341 - Pf6N2E2_1601 0.80 +0.3 1,874,341 - Pf6N2E2_1601 0.80 -1.0 1,874,341 - Pf6N2E2_1601 0.80 +0.3 1,874,341 - Pf6N2E2_1601 0.80 +0.3 1,874,341 - Pf6N2E2_1601 0.80 +0.3 1,874,341 - Pf6N2E2_1601 0.80 -0.8 1,874,341 - Pf6N2E2_1601 0.80 +0.3 1,874,341 - Pf6N2E2_1601 0.80 -0.7 1,874,341 - Pf6N2E2_1601 0.80 -0.7 1,874,343 - Pf6N2E2_1601 0.80 +0.3 1,874,391 + Pf6N2E2_1601 0.88 +0.3 1,874,391 + Pf6N2E2_1601 0.88 +0.3 1,874,392 - Pf6N2E2_1601 0.88 -0.7 1,874,508 - -0.6 1,874,543 + +0.3 1,874,543 + -0.2 1,874,543 + +0.3 1,874,766 + Pf6N2E2_1602 0.30 +0.3 1,874,766 + Pf6N2E2_1602 0.30 -0.7 1,874,768 + Pf6N2E2_1602 0.30 +0.3 1,874,768 + Pf6N2E2_1602 0.30 -2.1 1,874,831 + Pf6N2E2_1602 0.38 +0.3
Or see this region's nucleotide sequence