Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1398 and Pf6N2E2_1399 are separated by 54 nucleotides Pf6N2E2_1399 and Pf6N2E2_1400 are separated by 159 nucleotides Pf6N2E2_1400 and Pf6N2E2_1401 overlap by 32 nucleotides
Pf6N2E2_1398 - Rhodanese-related sulfurtransferase, at 1,638,215 to 1,638,748
_1398
Pf6N2E2_1399 - Transcriptional regulator, LuxR family, at 1,638,803 to 1,639,474
_1399
Pf6N2E2_1400 - Sensory box histidine kinase/response regulator, at 1,639,634 to 1,642,234
_1400
Pf6N2E2_1401 - FIG00955631: hypothetical protein, at 1,642,203 to 1,643,366
_1401
Position (kb)
1639
1640
1641
1642
1643 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1638.634 kb on - strand, within Pf6N2E2_1398 at 1638.657 kb on - strand, within Pf6N2E2_1398 at 1638.657 kb on - strand, within Pf6N2E2_1398 at 1638.668 kb on + strand, within Pf6N2E2_1398 at 1638.668 kb on + strand, within Pf6N2E2_1398 at 1638.792 kb on + strand at 1638.812 kb on + strand at 1638.812 kb on + strand at 1638.813 kb on - strand at 1638.813 kb on - strand at 1638.814 kb on + strand at 1639.097 kb on + strand, within Pf6N2E2_1399 at 1639.098 kb on - strand, within Pf6N2E2_1399 at 1639.098 kb on - strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.181 kb on + strand, within Pf6N2E2_1399 at 1639.182 kb on - strand, within Pf6N2E2_1399 at 1639.182 kb on - strand, within Pf6N2E2_1399 at 1639.460 kb on + strand at 1639.460 kb on + strand at 1639.460 kb on + strand at 1639.753 kb on - strand at 1639.753 kb on - strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.881 kb on + strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.882 kb on - strand at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.957 kb on + strand, within Pf6N2E2_1400 at 1639.958 kb on - strand, within Pf6N2E2_1400 at 1639.958 kb on - strand, within Pf6N2E2_1400 at 1639.958 kb on - strand, within Pf6N2E2_1400 at 1640.240 kb on + strand, within Pf6N2E2_1400 at 1640.240 kb on + strand, within Pf6N2E2_1400 at 1640.267 kb on - strand, within Pf6N2E2_1400 at 1640.473 kb on - strand at 1640.530 kb on + strand, within Pf6N2E2_1400 at 1640.531 kb on - strand, within Pf6N2E2_1400 at 1640.975 kb on + strand, within Pf6N2E2_1400 at 1640.976 kb on - strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.979 kb on + strand, within Pf6N2E2_1400 at 1640.980 kb on - strand, within Pf6N2E2_1400 at 1640.980 kb on - strand, within Pf6N2E2_1400 at 1640.980 kb on - strand, within Pf6N2E2_1400 at 1640.994 kb on + strand, within Pf6N2E2_1400 at 1640.994 kb on + strand, within Pf6N2E2_1400 at 1640.995 kb on - strand, within Pf6N2E2_1400 at 1640.995 kb on - strand, within Pf6N2E2_1400 at 1640.995 kb on - strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.069 kb on + strand, within Pf6N2E2_1400 at 1641.070 kb on - strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on + strand, within Pf6N2E2_1400 at 1641.150 kb on - strand, within Pf6N2E2_1400 at 1641.151 kb on - strand, within Pf6N2E2_1400 at 1641.163 kb on - strand, within Pf6N2E2_1400 at 1641.204 kb on + strand, within Pf6N2E2_1400 at 1641.661 kb on + strand, within Pf6N2E2_1400 at 1641.735 kb on + strand, within Pf6N2E2_1400 at 1641.736 kb on - strand, within Pf6N2E2_1400 at 1642.112 kb on + strand at 1642.262 kb on + strand at 1642.263 kb on - strand at 1642.288 kb on + strand at 1642.288 kb on + strand at 1642.289 kb on - strand at 1642.289 kb on - strand at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.511 kb on - strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.558 kb on + strand, within Pf6N2E2_1401 at 1642.559 kb on - strand, within Pf6N2E2_1401 at 1642.559 kb on - strand, within Pf6N2E2_1401 at 1642.561 kb on + strand, within Pf6N2E2_1401 at 1642.648 kb on + strand, within Pf6N2E2_1401 at 1642.648 kb on + strand, within Pf6N2E2_1401 at 1642.649 kb on - strand, within Pf6N2E2_1401 at 1642.682 kb on - strand, within Pf6N2E2_1401 at 1642.829 kb on - strand, within Pf6N2E2_1401 at 1642.849 kb on + strand, within Pf6N2E2_1401 at 1642.850 kb on - strand, within Pf6N2E2_1401 at 1642.850 kb on - strand, within Pf6N2E2_1401 at 1643.230 kb on + strand, within Pf6N2E2_1401 at 1643.231 kb on - strand, within Pf6N2E2_1401
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 1,638,634 - Pf6N2E2_1398 0.78 -1.4 1,638,657 - Pf6N2E2_1398 0.83 -0.4 1,638,657 - Pf6N2E2_1398 0.83 +1.2 1,638,668 + Pf6N2E2_1398 0.85 -0.8 1,638,668 + Pf6N2E2_1398 0.85 +0.2 1,638,792 + +0.2 1,638,812 + -0.2 1,638,812 + +0.2 1,638,813 - +0.2 1,638,813 - +0.2 1,638,814 + +0.2 1,639,097 + Pf6N2E2_1399 0.44 +0.2 1,639,098 - Pf6N2E2_1399 0.44 +0.2 1,639,098 - Pf6N2E2_1399 0.44 +0.2 1,639,181 + Pf6N2E2_1399 0.56 +0.2 1,639,181 + Pf6N2E2_1399 0.56 -0.4 1,639,181 + Pf6N2E2_1399 0.56 +0.2 1,639,181 + Pf6N2E2_1399 0.56 -0.8 1,639,182 - Pf6N2E2_1399 0.56 -0.8 1,639,182 - Pf6N2E2_1399 0.56 -0.8 1,639,460 + +2.7 1,639,460 + +0.2 1,639,460 + +0.9 1,639,753 - +0.2 1,639,753 - -1.8 1,639,881 + -6.9 1,639,881 + +0.2 1,639,881 + +0.2 1,639,881 + -0.8 1,639,881 + -1.4 1,639,882 - -1.4 1,639,882 - +0.2 1,639,882 - +0.2 1,639,882 - +0.2 1,639,882 - +0.2 1,639,882 - -0.8 1,639,882 - +0.2 1,639,957 + Pf6N2E2_1400 0.12 -0.8 1,639,957 + Pf6N2E2_1400 0.12 +0.2 1,639,957 + Pf6N2E2_1400 0.12 +0.2 1,639,957 + Pf6N2E2_1400 0.12 +0.2 1,639,958 - Pf6N2E2_1400 0.12 -3.4 1,639,958 - Pf6N2E2_1400 0.12 +0.2 1,639,958 - Pf6N2E2_1400 0.12 -6.9 1,640,240 + Pf6N2E2_1400 0.23 -0.8 1,640,240 + Pf6N2E2_1400 0.23 -0.8 1,640,267 - Pf6N2E2_1400 0.24 -2.6 1,640,473 - -4.0 1,640,530 + Pf6N2E2_1400 0.34 -3.8 1,640,531 - Pf6N2E2_1400 0.34 +0.2 1,640,975 + Pf6N2E2_1400 0.52 -1.4 1,640,976 - Pf6N2E2_1400 0.52 +0.2 1,640,979 + Pf6N2E2_1400 0.52 -1.8 1,640,979 + Pf6N2E2_1400 0.52 -1.4 1,640,979 + Pf6N2E2_1400 0.52 -0.8 1,640,979 + Pf6N2E2_1400 0.52 +0.2 1,640,979 + Pf6N2E2_1400 0.52 -1.8 1,640,979 + Pf6N2E2_1400 0.52 +0.2 1,640,980 - Pf6N2E2_1400 0.52 -3.4 1,640,980 - Pf6N2E2_1400 0.52 -4.3 1,640,980 - Pf6N2E2_1400 0.52 +0.2 1,640,994 + Pf6N2E2_1400 0.52 -0.8 1,640,994 + Pf6N2E2_1400 0.52 -1.4 1,640,995 - Pf6N2E2_1400 0.52 +0.2 1,640,995 - Pf6N2E2_1400 0.52 +0.2 1,640,995 - Pf6N2E2_1400 0.52 -0.8 1,641,069 + Pf6N2E2_1400 0.55 +0.2 1,641,069 + Pf6N2E2_1400 0.55 +0.2 1,641,069 + Pf6N2E2_1400 0.55 +0.2 1,641,069 + Pf6N2E2_1400 0.55 +0.2 1,641,070 - Pf6N2E2_1400 0.55 -0.8 1,641,150 + Pf6N2E2_1400 0.58 -1.8 1,641,150 + Pf6N2E2_1400 0.58 +0.2 1,641,150 + Pf6N2E2_1400 0.58 +0.2 1,641,150 + Pf6N2E2_1400 0.58 -3.6 1,641,150 - Pf6N2E2_1400 0.58 -1.4 1,641,151 - Pf6N2E2_1400 0.58 -3.6 1,641,163 - Pf6N2E2_1400 0.59 -2.5 1,641,204 + Pf6N2E2_1400 0.60 +0.2 1,641,661 + Pf6N2E2_1400 0.78 +0.2 1,641,735 + Pf6N2E2_1400 0.81 -0.8 1,641,736 - Pf6N2E2_1400 0.81 +0.2 1,642,112 + +0.2 1,642,262 + +0.2 1,642,263 - -1.1 1,642,288 + +0.5 1,642,288 + +0.2 1,642,289 - +0.2 1,642,289 - +0.2 1,642,511 - Pf6N2E2_1401 0.26 +0.2 1,642,511 - Pf6N2E2_1401 0.26 -1.3 1,642,511 - Pf6N2E2_1401 0.26 -0.8 1,642,511 - Pf6N2E2_1401 0.26 +0.2 1,642,511 - Pf6N2E2_1401 0.26 -2.0 1,642,558 + Pf6N2E2_1401 0.30 +0.2 1,642,558 + Pf6N2E2_1401 0.30 +0.2 1,642,558 + Pf6N2E2_1401 0.30 +0.2 1,642,558 + Pf6N2E2_1401 0.30 -2.1 1,642,558 + Pf6N2E2_1401 0.30 -2.6 1,642,558 + Pf6N2E2_1401 0.30 -0.8 1,642,559 - Pf6N2E2_1401 0.31 +0.2 1,642,559 - Pf6N2E2_1401 0.31 -0.8 1,642,561 + Pf6N2E2_1401 0.31 +0.2 1,642,648 + Pf6N2E2_1401 0.38 -2.8 1,642,648 + Pf6N2E2_1401 0.38 -0.8 1,642,649 - Pf6N2E2_1401 0.38 +0.2 1,642,682 - Pf6N2E2_1401 0.41 +0.2 1,642,829 - Pf6N2E2_1401 0.54 +0.2 1,642,849 + Pf6N2E2_1401 0.55 +0.2 1,642,850 - Pf6N2E2_1401 0.56 -0.8 1,642,850 - Pf6N2E2_1401 0.56 -0.8 1,643,230 + Pf6N2E2_1401 0.88 +0.2 1,643,231 - Pf6N2E2_1401 0.88 -6.8
Or see this region's nucleotide sequence