Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_847 and Pf6N2E2_848 overlap by 8 nucleotides Pf6N2E2_848 and Pf6N2E2_849 overlap by 19 nucleotides Pf6N2E2_849 and Pf6N2E2_850 are separated by 282 nucleotides Pf6N2E2_850 and Pf6N2E2_851 are separated by 96 nucleotides
Pf6N2E2_847 - Phosphoribosyl transferase domain protein, at 992,337 to 993,041
_847
Pf6N2E2_848 - hypothetical protein, at 993,034 to 993,729
_848
Pf6N2E2_849 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 993,711 to 995,078
_849
Pf6N2E2_850 - hypothetical protein, at 995,361 to 995,474
_850
Pf6N2E2_851 - Osmotically inducible protein Y precursor, at 995,571 to 996,218
_851
Position (kb) 993
994
995
996 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 992.798 kb on - strand, within Pf6N2E2_847 at 992.934 kb on + strand, within Pf6N2E2_847 at 992.934 kb on + strand, within Pf6N2E2_847 at 992.935 kb on - strand, within Pf6N2E2_847 at 993.172 kb on + strand, within Pf6N2E2_848 at 993.172 kb on + strand, within Pf6N2E2_848 at 993.173 kb on - strand, within Pf6N2E2_848 at 993.173 kb on - strand, within Pf6N2E2_848 at 993.670 kb on + strand at 993.671 kb on - strand at 993.671 kb on - strand at 993.671 kb on - strand at 993.705 kb on - strand at 993.705 kb on - strand at 993.705 kb on - strand at 993.727 kb on + strand at 993.728 kb on - strand at 993.874 kb on + strand, within Pf6N2E2_849 at 993.874 kb on + strand, within Pf6N2E2_849 at 994.115 kb on - strand, within Pf6N2E2_849 at 994.318 kb on + strand, within Pf6N2E2_849 at 994.319 kb on - strand, within Pf6N2E2_849 at 994.321 kb on + strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.483 kb on + strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.523 kb on - strand, within Pf6N2E2_849 at 994.570 kb on + strand, within Pf6N2E2_849 at 994.570 kb on + strand, within Pf6N2E2_849 at 994.607 kb on - strand, within Pf6N2E2_849 at 994.771 kb on + strand, within Pf6N2E2_849 at 994.771 kb on + strand, within Pf6N2E2_849 at 994.772 kb on - strand, within Pf6N2E2_849 at 994.772 kb on - strand, within Pf6N2E2_849 at 994.870 kb on - strand, within Pf6N2E2_849 at 994.873 kb on + strand, within Pf6N2E2_849 at 994.873 kb on + strand, within Pf6N2E2_849 at 994.874 kb on - strand, within Pf6N2E2_849 at 994.874 kb on - strand, within Pf6N2E2_849 at 995.018 kb on + strand at 995.019 kb on - strand at 995.029 kb on + strand at 995.030 kb on - strand at 995.041 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.125 kb on + strand at 995.126 kb on - strand at 995.126 kb on - strand at 995.126 kb on - strand at 995.215 kb on + strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.220 kb on - strand at 995.225 kb on + strand at 995.226 kb on - strand at 995.290 kb on - strand at 995.297 kb on + strand at 995.323 kb on - strand at 995.362 kb on + strand at 995.739 kb on - strand, within Pf6N2E2_851 at 995.864 kb on - strand, within Pf6N2E2_851 at 995.928 kb on + strand, within Pf6N2E2_851 at 995.929 kb on - strand, within Pf6N2E2_851 at 995.929 kb on - strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.015 kb on + strand, within Pf6N2E2_851 at 996.016 kb on - strand, within Pf6N2E2_851 at 996.016 kb on - strand, within Pf6N2E2_851 at 996.016 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 992,798 - Pf6N2E2_847 0.65 +0.2 992,934 + Pf6N2E2_847 0.85 +0.2 992,934 + Pf6N2E2_847 0.85 -0.3 992,935 - Pf6N2E2_847 0.85 -1.4 993,172 + Pf6N2E2_848 0.20 +0.2 993,172 + Pf6N2E2_848 0.20 -0.6 993,173 - Pf6N2E2_848 0.20 +0.2 993,173 - Pf6N2E2_848 0.20 +0.2 993,670 + +0.2 993,671 - -0.8 993,671 - -0.5 993,671 - -0.8 993,705 - +0.2 993,705 - -0.8 993,705 - +0.2 993,727 + -0.1 993,728 - -0.8 993,874 + Pf6N2E2_849 0.12 -0.2 993,874 + Pf6N2E2_849 0.12 +0.2 994,115 - Pf6N2E2_849 0.30 -0.4 994,318 + Pf6N2E2_849 0.44 +0.2 994,319 - Pf6N2E2_849 0.44 +0.2 994,321 + Pf6N2E2_849 0.45 +0.2 994,322 - Pf6N2E2_849 0.45 +0.2 994,322 - Pf6N2E2_849 0.45 +1.2 994,322 - Pf6N2E2_849 0.45 +0.2 994,322 - Pf6N2E2_849 0.45 +0.2 994,483 + Pf6N2E2_849 0.56 +0.2 994,484 - Pf6N2E2_849 0.57 +0.2 994,484 - Pf6N2E2_849 0.57 -0.8 994,484 - Pf6N2E2_849 0.57 +0.2 994,484 - Pf6N2E2_849 0.57 -0.8 994,523 - Pf6N2E2_849 0.59 +0.2 994,570 + Pf6N2E2_849 0.63 -0.6 994,570 + Pf6N2E2_849 0.63 -0.8 994,607 - Pf6N2E2_849 0.65 -0.3 994,771 + Pf6N2E2_849 0.77 +0.2 994,771 + Pf6N2E2_849 0.77 +0.2 994,772 - Pf6N2E2_849 0.78 +0.2 994,772 - Pf6N2E2_849 0.78 -0.8 994,870 - Pf6N2E2_849 0.85 +0.2 994,873 + Pf6N2E2_849 0.85 -0.8 994,873 + Pf6N2E2_849 0.85 +0.2 994,874 - Pf6N2E2_849 0.85 -0.1 994,874 - Pf6N2E2_849 0.85 +0.2 995,018 + +0.2 995,019 - -0.9 995,029 + +0.2 995,030 - -0.8 995,041 + +0.2 995,125 + +1.0 995,125 + +0.2 995,125 + -0.8 995,125 + -1.4 995,126 - +0.2 995,126 - +0.2 995,126 - -0.4 995,215 + +0.2 995,219 + -2.8 995,219 + -1.4 995,219 + +0.2 995,220 - -0.8 995,225 + +0.2 995,226 - -1.9 995,290 - -0.8 995,297 + +0.8 995,323 - +0.2 995,362 + +0.2 995,739 - Pf6N2E2_851 0.26 +0.2 995,864 - Pf6N2E2_851 0.45 -0.8 995,928 + Pf6N2E2_851 0.55 -1.0 995,929 - Pf6N2E2_851 0.55 -0.5 995,929 - Pf6N2E2_851 0.55 +0.2 996,015 + Pf6N2E2_851 0.69 +0.2 996,015 + Pf6N2E2_851 0.69 +0.2 996,015 + Pf6N2E2_851 0.69 +0.3 996,016 - Pf6N2E2_851 0.69 +0.2 996,016 - Pf6N2E2_851 0.69 -0.2 996,016 - +0.2
Or see this region's nucleotide sequence