Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_659 and Pf6N2E2_660 are separated by 223 nucleotides Pf6N2E2_660 and Pf6N2E2_661 are separated by 168 nucleotides Pf6N2E2_661 and Pf6N2E2_662 are separated by 242 nucleotides Pf6N2E2_662 and Pf6N2E2_663 are separated by 4 nucleotides
Pf6N2E2_659 - Transcriptional regulatory protein ompR, at 763,572 to 764,312
_659
Pf6N2E2_660 - hypothetical protein, at 764,536 to 764,799
_660
Pf6N2E2_661 - DipZ protein, at 764,968 to 766,179
_661
Pf6N2E2_662 - Valyl-tRNA synthetase, at 766,422 to 766,739
_662
Pf6N2E2_663 - Transcriptional activator of acetoin dehydrogenase operon AcoR, at 766,744 to 768,609
_663
Position (kb) 764
765
766
767 Strain fitness (log2 ratio) -2
-1
0
1
2 at 764.006 kb on - strand, within Pf6N2E2_659 at 764.006 kb on - strand, within Pf6N2E2_659 at 764.074 kb on - strand, within Pf6N2E2_659 at 764.246 kb on - strand at 764.286 kb on + strand at 764.287 kb on - strand at 764.287 kb on - strand at 764.349 kb on + strand at 764.384 kb on + strand at 764.385 kb on - strand at 764.385 kb on - strand at 764.385 kb on - strand at 764.385 kb on - strand at 764.394 kb on + strand at 764.395 kb on - strand at 764.510 kb on + strand at 764.511 kb on - strand at 764.554 kb on + strand at 764.555 kb on - strand at 764.632 kb on + strand, within Pf6N2E2_660 at 764.632 kb on + strand, within Pf6N2E2_660 at 764.633 kb on - strand, within Pf6N2E2_660 at 764.725 kb on + strand, within Pf6N2E2_660 at 764.726 kb on - strand, within Pf6N2E2_660 at 764.938 kb on + strand at 765.682 kb on + strand, within Pf6N2E2_661 at 765.682 kb on + strand, within Pf6N2E2_661 at 765.682 kb on + strand, within Pf6N2E2_661 at 765.683 kb on - strand, within Pf6N2E2_661 at 765.683 kb on - strand, within Pf6N2E2_661 at 765.683 kb on - strand, within Pf6N2E2_661 at 765.683 kb on - strand, within Pf6N2E2_661 at 765.835 kb on + strand, within Pf6N2E2_661 at 765.835 kb on + strand, within Pf6N2E2_661 at 765.836 kb on - strand, within Pf6N2E2_661 at 765.868 kb on + strand, within Pf6N2E2_661 at 765.869 kb on - strand, within Pf6N2E2_661 at 765.937 kb on + strand, within Pf6N2E2_661 at 765.977 kb on + strand, within Pf6N2E2_661 at 765.998 kb on - strand, within Pf6N2E2_661 at 766.051 kb on + strand, within Pf6N2E2_661 at 766.066 kb on + strand at 766.067 kb on - strand at 766.321 kb on + strand at 766.570 kb on - strand, within Pf6N2E2_662 at 766.653 kb on + strand, within Pf6N2E2_662 at 766.653 kb on + strand, within Pf6N2E2_662 at 766.654 kb on - strand, within Pf6N2E2_662 at 766.654 kb on - strand, within Pf6N2E2_662 at 766.654 kb on - strand, within Pf6N2E2_662 at 766.698 kb on + strand, within Pf6N2E2_662 at 766.698 kb on + strand, within Pf6N2E2_662 at 766.698 kb on + strand, within Pf6N2E2_662 at 766.699 kb on - strand, within Pf6N2E2_662 at 766.701 kb on + strand, within Pf6N2E2_662 at 766.701 kb on + strand, within Pf6N2E2_662 at 766.702 kb on - strand, within Pf6N2E2_662 at 766.702 kb on - strand, within Pf6N2E2_662 at 766.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 764,006 - Pf6N2E2_659 0.59 +0.2 764,006 - Pf6N2E2_659 0.59 +0.2 764,074 - Pf6N2E2_659 0.68 +0.2 764,246 - -1.2 764,286 + +0.2 764,287 - -0.8 764,287 - +0.2 764,349 + +0.2 764,384 + -0.4 764,385 - +1.8 764,385 - -1.6 764,385 - -0.1 764,385 - +0.2 764,394 + +0.2 764,395 - +0.2 764,510 + +0.2 764,511 - +0.2 764,554 + +0.2 764,555 - -0.4 764,632 + Pf6N2E2_660 0.36 -1.4 764,632 + Pf6N2E2_660 0.36 +0.2 764,633 - Pf6N2E2_660 0.37 +0.2 764,725 + Pf6N2E2_660 0.72 +0.2 764,726 - Pf6N2E2_660 0.72 +1.8 764,938 + +0.2 765,682 + Pf6N2E2_661 0.59 +0.2 765,682 + Pf6N2E2_661 0.59 -0.1 765,682 + Pf6N2E2_661 0.59 +0.2 765,683 - Pf6N2E2_661 0.59 +0.2 765,683 - Pf6N2E2_661 0.59 -1.4 765,683 - Pf6N2E2_661 0.59 -0.8 765,683 - Pf6N2E2_661 0.59 +0.2 765,835 + Pf6N2E2_661 0.72 -0.6 765,835 + Pf6N2E2_661 0.72 +1.2 765,836 - Pf6N2E2_661 0.72 +0.3 765,868 + Pf6N2E2_661 0.74 -0.4 765,869 - Pf6N2E2_661 0.74 +0.2 765,937 + Pf6N2E2_661 0.80 +2.2 765,977 + Pf6N2E2_661 0.83 -0.8 765,998 - Pf6N2E2_661 0.85 -0.8 766,051 + Pf6N2E2_661 0.89 -0.8 766,066 + +0.2 766,067 - +0.2 766,321 + -0.7 766,570 - Pf6N2E2_662 0.47 +0.2 766,653 + Pf6N2E2_662 0.73 +0.2 766,653 + Pf6N2E2_662 0.73 -0.8 766,654 - Pf6N2E2_662 0.73 +0.2 766,654 - Pf6N2E2_662 0.73 +0.2 766,654 - Pf6N2E2_662 0.73 +0.2 766,698 + Pf6N2E2_662 0.87 +0.2 766,698 + Pf6N2E2_662 0.87 -1.9 766,698 + Pf6N2E2_662 0.87 +0.2 766,699 - Pf6N2E2_662 0.87 +0.2 766,701 + Pf6N2E2_662 0.88 +0.2 766,701 + Pf6N2E2_662 0.88 +0.2 766,702 - Pf6N2E2_662 0.88 +0.0 766,702 - Pf6N2E2_662 0.88 +0.2 766,738 - -1.8
Or see this region's nucleotide sequence