Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_629 and Pf6N2E2_630 overlap by 8 nucleotides Pf6N2E2_630 and Pf6N2E2_631 are separated by 63 nucleotides Pf6N2E2_631 and Pf6N2E2_632 are separated by 287 nucleotides
Pf6N2E2_629 - 2-ketogluconate kinase (EC 2.7.1.13), at 716,617 to 717,591
_629
Pf6N2E2_630 - Epimerase KguE, at 717,584 to 718,366
_630
Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455
_631
Pf6N2E2_632 - hypothetical protein, at 719,743 to 721,851
_632
Position (kb) 718
719
720 Strain fitness (log2 ratio) -2
-1
0
1
2 at 717.698 kb on + strand, within Pf6N2E2_630 at 717.699 kb on - strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 718.071 kb on - strand, within Pf6N2E2_630 at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.371 kb on - strand at 718.371 kb on - strand at 718.373 kb on + strand at 718.373 kb on + strand at 718.634 kb on + strand, within Pf6N2E2_631 at 718.634 kb on + strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.472 kb on - strand at 719.472 kb on - strand at 719.481 kb on - strand at 719.481 kb on - strand at 719.481 kb on - strand at 719.484 kb on - strand at 719.736 kb on - strand at 719.773 kb on + strand at 719.773 kb on + strand at 719.774 kb on - strand at 719.774 kb on - strand at 719.776 kb on + strand at 719.776 kb on + strand at 719.777 kb on - strand at 719.805 kb on - strand at 719.897 kb on - strand at 720.008 kb on - strand, within Pf6N2E2_632 at 720.187 kb on + strand, within Pf6N2E2_632 at 720.188 kb on - strand, within Pf6N2E2_632 at 720.377 kb on + strand, within Pf6N2E2_632
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 717,698 + Pf6N2E2_630 0.15 +0.2 717,699 - Pf6N2E2_630 0.15 -0.0 717,813 + Pf6N2E2_630 0.29 +0.2 717,813 + Pf6N2E2_630 0.29 +0.2 717,813 + Pf6N2E2_630 0.29 +0.2 717,814 - Pf6N2E2_630 0.29 +0.2 717,814 - Pf6N2E2_630 0.29 +0.2 718,071 - Pf6N2E2_630 0.62 -0.8 718,370 + +0.2 718,370 + +0.2 718,370 + +0.2 718,370 + -0.8 718,370 + -1.4 718,371 - -0.7 718,371 - -0.2 718,373 + -0.8 718,373 + +0.2 718,634 + Pf6N2E2_631 0.20 -0.0 718,634 + Pf6N2E2_631 0.20 +0.2 718,635 - Pf6N2E2_631 0.20 +1.8 718,635 - Pf6N2E2_631 0.20 +1.4 719,083 - Pf6N2E2_631 0.64 +1.0 719,083 - Pf6N2E2_631 0.64 +0.2 719,083 - Pf6N2E2_631 0.64 +0.2 719,152 + Pf6N2E2_631 0.70 -1.8 719,152 + Pf6N2E2_631 0.70 +0.2 719,153 - Pf6N2E2_631 0.70 -0.8 719,153 - Pf6N2E2_631 0.70 +0.2 719,153 - Pf6N2E2_631 0.70 -0.4 719,472 - +0.2 719,472 - +0.2 719,481 - +0.2 719,481 - +0.2 719,481 - +0.2 719,484 - +0.2 719,736 - +0.2 719,773 + +0.2 719,773 + +0.2 719,774 - -0.8 719,774 - -0.8 719,776 + -0.8 719,776 + -0.8 719,777 - +0.2 719,805 - +0.2 719,897 - -0.8 720,008 - Pf6N2E2_632 0.13 +0.8 720,187 + Pf6N2E2_632 0.21 -0.8 720,188 - Pf6N2E2_632 0.21 -0.7 720,377 + Pf6N2E2_632 0.30 -0.8
Or see this region's nucleotide sequence